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Recombinant Mouse Wnt-9b Protein

Catalog #: 3669-WN
9 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
3669-WN-025/CF

With Carrier

Catalog # Availability Size / Price Qty
3669-WN-025
R&D Systems Recombinant Proteins and Enzymes
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Citations (9)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Wnt-9b Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The typical ED50 range is 100-400 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse Wnt-9b protein
Ala24-Arg359
Accession #
O35468
N-terminal Sequence
Analysis
Ala24
Predicted Molecular Mass
36.8 kDa
SDS-PAGE
39-41 kDa, reducing conditions

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3669-WN (with carrier)

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3669-WN/CF (carrier free)

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COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3669-WN

Formulation Lyophilized from a 0.2 μm filtered solution in PBS, NaCl, EDTA and CHAPS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.

3669-WN/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS, NaCl, EDTA and CHAPS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Wnt-9b

Mouse Wnt-9b, previously called Wnt-14b or Wnt-15, is one of about 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved, cysteine-rich secreted glycoproteins important for normal developmental processes (1-3). Wnts mainly transduce signals by binding to receptors of the Frizzled family, in conjunction with a coreceptor of the low-density lipoprotein receptor-related protein family (LRP-5 or -6) (1, 2). The 359 aa mouse Wnt-9b precursor contains a 336 aa mature region with 24 conserved cysteines (3). Mature mouse Wnt-9b shares 93%, 98%, 92%, 92% and 91% aa identity with human, rat, canine, equine and bovine Wnt-9b, respectively. It is evolutionarily related to Wnt-9a and Wnt-3, which share 63% and 32% aa identity, respectively (4). Wnt-9b mRNA is expressed in late embryos and in adult kidney, with lesser amounts in brain and liver (3, 4). It appears to direct mesenchymal-to-epithelial transition in the kidney and other tissues (5). During kidney development, it is expressed in the ureteric bud and induces mesonephric and metanephric tubulogenesis, nephron development, and caudal extension of the Mullerian duct, acting upstream of Wnt-4 (5-7). Induction is dependent on beta -catenin activity, implicating the canonical signaling pathway (7). Mice devoid of Wnt-9b die shortly after birth due to kidney agenesis, while low expression results in small kidneys with fewer nephrons (1, 5, 6). Mutations of Wnt-9b also cause incompletely penetrant cleft lip and palate in mice, indicating its involvement with facial midline morphogenesis (8, 9). It has weak transforming activity compared to other transforming Wnts (4).

References
  1. www.stanford.edu/~rnusse/wntwindow.html
  2. Logan, C. Y. and R. Nusse (2004) Annu. Rev. Cell Dev. Biol. 20:781.
  3. Kirikoshi, H. and  M. Katoh (2002) Int. J. Mol. Med. 9:135.
  4. Qian, J. et al. (2003) Genomics 81:34.
  5. Carroll, T. J. et al. (2005) Dev. Cell 9:283.
  6. Merkel, C. E. et al. (2007) Pediatr. Nephrol. 22:1825.
  7. Park, J-S. et al. (2007) Development 134:2533.
  8. Lan, Y. et al. (2006) Dev. Dyn. 235:1448.
  9. Juriloff, D. M. and  M. J. Harris (2008) Birth Defects Res. A Clin. Mol. Teratol. 82:63.
Long Name
Wingless-type MMTV Integration Site Family, Member 9b
Entrez Gene IDs
7484 (Human); 22412 (Mouse); 303586 (Rat)
Alternate Names
Protein Wnt-14b; wingless-type MMTV integration site family, member 15; wingless-type MMTV integration site family, member 9B; Wnt-14b; WNT14Bprotein Wnt-9b; Wnt-15; WNT15Protein Wnt-15; Wnt9b; Wnt-9b

Citations for Recombinant Mouse Wnt-9b Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. In�vitro atlas of dorsal spinal interneurons reveals Wnt signaling as a critical regulator of progenitor expansion
    Authors: S Gupta, R Kawaguchi, E Heinrichs, S Gallardo, S Castellano, I Mandric, BG Novitch, SJ Butler
    Oncogene, 2022-07-19;40(3):111119.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. WNT3A Promotes Neuronal Regeneration upon Traumatic Brain Injury
    Authors: CY Chang, MZ Liang, CC Wu, PY Huang, HI Chen, SF Yet, JW Tsai, CF Kao, L Chen
    Int J Mol Sci, 2020-02-21;21(4):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Cell Culture
  3. Molecular determinants of WNT9b responsiveness in nephron progenitor cells
    Authors: KK Dickinson, LC Hammond, CM Karner, ND Hastie, TJ Carroll, P Goodyer
    PLoS ONE, 2019-04-12;14(4):e0215139.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Assessment of Frizzled 6 membrane mobility by FRAP supports G protein coupling and reveals WNT-Frizzled selectivity.
    Authors: Kilander M, Dahlstrom J, Schulte G
    Cell Signal, 2014-05-27;26(9):1943-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Noncanonical Wnt5a enhances Wnt/beta-catenin signaling during osteoblastogenesis.
    Authors: Okamoto M, Udagawa N, Uehara S, Maeda K, Yamashita T, Nakamichi Y, Kato H, Saito N, Minami Y, Takahashi N, Kobayashi Y
    Sci Rep, 2014-03-27;4(0):4493.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Activation of intracellular calcium by multiple Wnt ligands and translocation of beta-catenin into the nucleus: a convergent model of Wnt/Ca2+ and Wnt/beta-catenin pathways.
    Authors: Thrasivoulou C, Millar M, Ahmed A
    J Biol Chem, 2013-10-24;288(50):35651-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Both LRP5 and LRP6 receptors are required to respond to physiological Wnt ligands in mammary epithelial cells and fibroblasts.
    Authors: Goel S, Chin EN, Fakhraldeen SA
    J. Biol. Chem., 2012-03-20;287(20):16454-66.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Wnt isoform-specific interactions with coreceptor specify inhibition or potentiation of signaling by LRP6 antibodies.
    Authors: Gong Y, Bourhis E, Chiu C, Stawicki S, DeAlmeida VI, Liu BY, Phamluong K, Cao TC, Carano RA, Ernst JA, Solloway M, Rubinfeld B, Hannoush RN, Wu Y, Polakis P, Costa M
    PLoS ONE, 2010-09-13;5(9):e12682.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Binding Assay
  9. Reconstitution of a frizzled8.Wnt3a.LRP6 signaling complex reveals multiple Wnt and Dkk1 binding sites on LRP6.
    Authors: Bourhis E, Tam C, Franke Y, Bazan JF, Ernst J, Hwang J, Costa M, Cochran AG, Hannoush RN
    J. Biol. Chem., 2010-01-21;285(12):9172-9.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Binding Assay

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