Recombinant Mouse Wnt-1/sFRP-1 Complex Protein

Catalog #: 9765-WN Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9765-WN-010

Recombinant Mouse Wnt-1/sFRP-1 Complex Protein Bioactivity

1 Image

Product Details

Supplemental Products

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Wnt-1/sFRP-1 Complex Protein Summary

Product Specifications

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to activate Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED₅₀ for this effect is 0.03-0.18 μg/mL

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Wnt-1 protein

Mouse Wnt-1
(Ala28-Leu370)
Accession # P04426

Mouse sFRP-1 (Ser32-Lys314) Accession # Q8C4U3
N-terminus	C-terminus

Accession #

P04426, Q8C4U3

N-terminal Sequence
Analysis

Ala28 (Wnt-1) & Ser32, Phe39 (sFRP-1)

Structure / Form

Non-covalent complex

Predicted Molecular Mass

38 kDa (Wnt-1) & 33 kDa (sFRP-1)

SDS-PAGE

35-59 kDa, reducing conditions

Product Datasheets

9765-WN

Product Datasheet

COA

SDS

9765-WN

Formulation	Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl, Trehalose and with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

View Larger

Recombinant Mouse Wnt-1/sFRP-1 Complex (Catalog # 9765-WN) activates Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED₅₀ for this effect is 0.03-0.18 μg/mL.

Reconstitution Calculator

Background: Wnt-1

Wnt-1 is one of 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins that are important for normal developmental processes (1). Mouse Wnt-1 cDNA encodes a 370 amino acid (aa) protein that contains four potential N-linked glycosylation sites plus 23 cysteines (2, 3). When secreted, it exists as a 343 aa glycoprotein with a molecular weight of 42 to 48 kDa. Mouse Wnt -1 shares a 99.7% aa sequence identity with human and rat Wnt-1. Wnts bind to the cell surface Frizzled family receptors in conjunction with low-density lipoprotein receptor-related protein family receptors (LRP5 or 6) resulting in the stabilization of intracellular beta -catenin levels (4). As intracellular beta -catenin levels rise, beta -catenin binds to TCF/LEF transcription factors leading to expression of Wnt target genes (5). Wnt-1 is known to bind to sFRPs, a family of secreted molecules that contain an N-terminal cysteine-rich domain (CRD) highly similar to the CRDs of the Frizzled family receptors (6,7). More than one molecule of sFRP is suggested to participate in the interaction with each Wnt molecule (7). R&D Systems produces biologically active mouse Wnt-1 protein in a complex with mouse sFRP-1, and this Wnt-1/sFRP-1 complex is stable in the physiological buffer without detergent.

References

Willert, K. and R. Nusse (2012) Cold Spring Harb. Perspect. Biol. 4:a007864.
Van Ooyen, A. et al. (1985) EMBO J. 4:2905.
Burrus, L.W. and A.P. McMahon (1995) Exp. Cell Res. 220:363.
MacDonald, B.T. and X. He (2012) Cold Spring Harb. Perspect. Biol. 4:a007880.
Korinek, V. et al. (1997) Science 275:1784.
Dennis, S. et al. (1999) J. Cell Sci. 112:3815.
Bafico, A. et al. (1999) J. Biol. Chem. 274:16180.

Long Name

Wingless-type MMTV Integration Site Family, Member 1

Entrez Gene IDs

7471 (Human); 22408 (Mouse)

Alternate Names

Int-1; INT1Proto-oncogene Int-1 homolog; proto-oncogene Wnt-1; wingless-type MMTV integration site family, member 1 (oncogene INT1); wingless-type MMTV integration site family, member 1; Wnt1; Wnt-1