Recombinant Mouse VSTM2B Fc Chimera Protein, CF

Catalog #: 10076-VT Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10076-VT-050

Recombinant Mouse VSTM2B Fc Chimera Protein Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse VSTM2B Fc Chimera Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IFN-gamma secretion by human peripheral blood mononuclear cells (PBMC). The ED₅₀ for this effect is 2-10 μg/mL.

Source

Mouse myeloma cell line, NS0-derived mouse VSTM2B protein

Mouse VSTM2B (Thr29-Ser263) Accession # Q9JME9	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q9JME9

N-terminal Sequence
Analysis

Thr29

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

51 kDa

SDS-PAGE

61-70 kDa, reducing conditions

Product Datasheets

10076-VT

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10076-VT

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Mouse VSTM2B Fc Chimera (Catalog # 10076-VT) inhibits IFN-gamma secretion by human peripheral blood mononuclear cells in the presence of anti-CD3 antibody. The ED₅₀ for this effect is 2-10 μg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Mouse VSTM2B Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 61-70 kDa and 120-140 kDa, respectively.

Reconstitution Calculator

Background: VSTM2B

V-set and transmembrane domain-containing protein 2B, also known as VSTM-2B, is a single pass Type I membrane protein. Mouse VSTM-2B contains an N-Terminal signal peptide (1-28 aa), an extracellular domain (aa 29-263) which contains a single Ig-like V-Type domain (aa 29-143) with a single disulfide bond between C49 and C127, a helical transmembrane domain (aa 264-284) and a single histidine in the cytoplasm (aa 285). VSTM2B is expressed in multiple organs, with the highest expression in the pyloric antrum. The extracellular domain shares 89% and 96% sequence identity for the human and rat homolog, respectively. Currently, both the expression pattern and biological functions of VSTM2B remain unknown. our in house data show that VSTM2B inhibits anti-CD3 induced IL-2 and IFN-g secretion on human T cells.

Long Name

V-Set And Transmembrane Domain Containing 2B

Entrez Gene IDs

342865 (Human); 58188 (Mouse); 361560 (Rat)

Alternate Names

V-set and transmembrane domain-containing protein 2B; VSTM2B