Recombinant Mouse TSLPR Fc Chimera Protein, CF

Catalog #: 546-TR
2 Citations Datasheet / COA / SDS

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546-TR-050

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Citations (2)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse TSLPR Fc Chimera Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit TSLP-dependent proliferation of BaF3 mouse pro‑B cells transfected with mouse IL-7 R alpha. The ED₅₀ for this effect is 2-8 µg/mL in the presence of 7.5 ng/mL of rmTSLP.

Source

Mouse myeloma cell line, NS0-derived mouse TSLP R protein

Mouse TSLP R (Ala20 - Leu233) Accession # BAA92159	DIEGRMD	Human IgG₁ (Pro100 - Lys330)
N-terminus		C-terminus

Accession #

BAA92159

N-terminal Sequence
Analysis

Ala20

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

49.3 kDa (monomer)

SDS-PAGE

65 - 80 kDa, reducing conditions

Product Datasheets

546-TR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

546-TR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: TSLPR

TSLP R, also named Delta (1) and CRLM-2 (2) (cytokine receptor-like module-2), was originally cloned as a novel type 1 cytokine receptor with similarity to the common gamma chain. It was subsequently identified to be a subunit of the cellular receptor for the IL-7-like cytokine TSLP and termed TSLP R (3). The TSLP R cDNA encodes a 359 amino acid (aa) residue type 1 membrane protein with a 24 aa residue signal peptide, a 206 aa residue extracellular domain that showed 24% sequence identity with the mouse common gamma receptor, a 23 aa residue transmembrane domain, and a 106 aa residue cytoplasmic domain. The cytoplasmic domain of TSLP R contains a membrane-proximal box1 motif which is known to be important for association with JAKs. An alternatively spliced mRNA variant encoding a soluble TSLP R has also been reported (2). TSLP R expression is ubiquitous in the immune and hematopoietic cells, but is up-regulated in Th2-skewed cells. Cells expressing TSLP R alone bind TSLP with low affinity. Co-expression of TSLP R and IL-7 R alpha is required for high-affinity TSLP binding and signal transduction (3, 4).

References

Fujio, K. et al. (2000) Blood 95:2204.
Hiroyama, T. et al. (2000) Biochem. Biophys. Res. Commun. 272:224.
Park, L.S. et al. (2000) J. Exp. Med. 192:659.
Pandey, A. et al. (2000) Nat. Immunol. 1:59.

Long Name

Thymic Stromal Lymphopoietin Receptor

Entrez Gene IDs

64109 (Human); 57914 (Mouse); 171499 (Rat)

Alternate Names

CRL2; CRL2cytokine receptor CRL2 precusor; CRLF2; CRLF2Y; CRLM-2; Cytokine receptor-like 2; cytokine receptor-like factor 2; ILXR; IL-XR; P2RY8/CRLF2 fusion; PCOR1; Thymic stromal lymphopoietin protein receptor; thymic stromal lymphopoietin receptor; thymic stromal-derived lymphopoietin receptor; TSLP R; TSLP receptor; TSLPR

Related Research Areas

Citations for Recombinant Mouse TSLPR Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Tissue signals imprint ILC2 identity with anticipatory function
Authors: RR Ricardo-Go, SJ Van Dyken, C Schneider, J Lee, JC Nussbaum, HE Liang, D Vaka, WL Eckalbar, AB Molofsky, DJ Erle, RM Locksley
Nat. Immunol., 2018-09-10;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
A potential role of thymic stromal lymphopoietin in the recruitment of macrophages to mouse intervertebral disc cells via monocyte chemotactic protein 1 induction: implications for herniated discs.
Authors: Ohba T, Haro H, Ando T, Koyama K, Hatsushika K, Suenaga F, Ohnuma Y, Nakamura Y, Katoh R, Ogawa H, Hamada Y, Nakao A
Arthritis Rheum., 2008-11-01;58(11):3510-9.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay