Recombinant Mouse TLR5 Fc Chimera Protein, CF

Catalog #: 7915-TR
2 Citations Datasheet / COA / SDS

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7915-TR-025

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse TLR5 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit Flagellin-induced IL-8 secretion in HT‑29 human colon adenocarcinoma cells. The ED₅₀ for this effect is 4-24 ng/mL

Source

Chinese Hamster Ovary cell line, CHO-derived mouse TLR5 protein

Mouse TLR5 (Ile21-Phe641) Accession # Q9JLF7	IEGRMDP	Mouse IgG_2A (Glu98-Lys330)
N-terminus		C-terminus

Accession #

Q9JLF7

N-terminal Sequence
Analysis

Ile21

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

97.3 kDa (monomer)

SDS-PAGE

100-125 kDa, reducing conditions

Product Datasheets

7915-TR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7915-TR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: TLR5

TLR5 (Toll-like receptor 5) is an approximately 100 kDa cell surface type I transmembrane glycoprotein of the TLR family of pathogen‑associated molecular pattern (PAMP) receptors (1‑3). TLR5 recognizes flagellins, proteins found on flagella of both gram‑positive and gram‑negative pathogenic bacteria (2, 3). The 859 amino acid (aa) mouse TLR5 precursor includes a 21 aa signal sequence, a 621 aa extracellular domain (ECD) with 9 potential N‑glycosylation sites and 16 leucine‑rich repeats (LRR) that contain the flagellin‑binding site, a transmembrane domain, and a 197 aa cytoplasmic region with a TIR (Toll/ IL-1 R) domain (1, 4). The mouse TLR5 ECD shares 84% aa sequence identity with rat and 69‑73% with human, feline, porcine and bovine TLR5. TLR5 is expressed on mucosal epithelia in the gastrointestinal tract, airways, and other areas of potential contact with bacteria, and on alveolar macrophages, monocytes, neutrophils, CD4⁺ T lymphocytes, and dendritic cell subsets (3‑7). In some portions of the intestine, epithelial TLR5 is expressed only on the basolateral surface (3, 4). Flagellin engagement induces signaling via direct interaction of TLR5 with MyD88, which activates NFkB and stimulates production of inflammatory cytokines such as TNF‑ alpha, IL‑1 beta, IL‑6 and IL‑8, depending on the cell type (2‑6, 8, 9). TLR5 allows recognition of pathogenic bacteria such as Pseudomonas, Salmonella and Listeria, without reaction to non‑flagellated commensal bacteria (3, 4). It stimulates massive neutrophil recruitment and EGF R‑mediated mucus production by airway epithelia (4, 9, 10). It promotes Th2 polarization and IgA secretion, and restrains local regulatory T cell generation (4, 7). Deletion of mouse TLR5 promotes colitis, probably due to TLR4‑mediated inappropriate responses to commensal bacteria (11).

References

Sebastiani, G. et al. (2000) Genomics 64:230.
Hayashi, F. et al. (2001) Nature 410:1099.
Gewirtz, A.T. et al. (2001) J. Immunol. 167:1882.
Rumbo, M. et al. (2006) FEBS Lett. 580:2976.
Descamps, D. et al. (2012) Proc. Natl. Acad. Sci. USA 109:16619.
Shibata, T. et al. (2012) Int. Immunol. 24:613.
Feng, T. et al. (2012) PLoS ONE 7:e35918.
Hawn, T.R. et al. (2003) J. Exp. Med. 198:1563.
Ben Mohamed, F. et al. (2012) PLoS ONE 7:e39888.
Yu, H. et al. (2012) Exp. Cell Res. 318:723.
Vijay-Kumar, M. et al. (2007) J. Clin. Invest. 117:3909.

Long Name

Toll-like Receptor 5

Entrez Gene IDs

7100 (Human); 53791 (Mouse); 289337 (Rat); 102118010 (Cynomolgus Monkey)

Alternate Names

MGC126430; MGC126431; SLEB1FLJ10052; TIL-3; TIL3Toll/interleukin-1 receptor-like protein 3; TLR5; toll-like receptor 5

Related Research Areas

Citations for Recombinant Mouse TLR5 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Flagellin attenuates experimental sepsis in a macrophage-dependent manner
Authors: X Yang, Y Yin, X Yan, Z Yu, Y Liu, J Cao
Crit Care, 2019-04-03;23(1):106.
Species: Mouse
Sample Types: In Vivo
Applications: Bioassay
Distinctive Recognition of Flagellin by Human and Mouse Toll-Like Receptor 5
PLoS ONE, 2016-07-08;11(7):e0158894.
Species: Human
Sample Types: Protein
Applications: Immunoprecipitation