Recombinant Mouse Siglec-H Fc Chimera Protein, CF

Catalog #: 10264-SH Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10264-SH-050

Recombinant Mouse Siglec-H Fc Chimera Protein SDS-PAGE

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Siglec-H Fc Chimera Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse Siglec‑H Fc Chimera (Catalog # 10264-SH) is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Mouse Siglec‑3 Fc Chimera (Catalog # 10102-SL) that produces 50% of the optimal binding response is 5-20 μg/mL.

Source

Mouse myeloma cell line, NS0-derived mouse Siglec-H protein

Mouse Siglec-H (Asn19-Gly269) Accession # NP_848821.2	IEGRMDP	Mouse IgG_2a (Glu98-Lys330)
N-terminus		C-terminus

Accession #

NP_848821.2

N-terminal Sequence
Analysis

Asn19

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

55 kDa

SDS-PAGE

71-81 kDa, under reducing conditions

Product Datasheets

10264-SH

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10264-SH

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SDS-PAGE

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2 μg/lane of Recombinant Mouse Siglec-H Fc Chimera (Catalog # 10264-SH) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie^®Blue staining, showing bands at 71-81 kDa and 140-160 kDa, respectively.

Reconstitution Calculator

Background: Siglec-H

Siglecs (sialic acid-binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily (1, 2). They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains (3, 4). Siglec 5 to 11 are collectively referred to as CD33-related Siglecs due to their high degree of sequence similarity with CD33/Siglec-3 both in their extracellular and intracellular regions. One remarkable feature of the CD33-related Siglecs is their differential expression pattern within the hematopoietic system (4, 5). Mouse Siglec-H is an endocytic receptor expressed predominantly on a specialized subset of leukocytes, the plasmacytoid dendritic cell (pDC). Siglec-H has not been shown to bind sialic acids (6). Unlike other CD33-related siglecs, the cytoplasmic tail of Siglec-H lacks ITIM-related sequence (6, 7). The transmembrane region contains a charged lysine residue that could associate with DAP12 which reduces interferon-alpha (IFN alpha ) production (6, 8).

References