Recombinant Mouse sFRP-3 Protein Summary
Product Specifications
Ala33-Ser323 with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
592-FR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
592-FR/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: sFRP-3
Secreted Frizzled Related Protein 3 (sFRP-3) was originally identified in bovine cartilage for its chondrogenic ability. Human, mouse, chick and Xenopus clones have also been isolated. sFRP-3 is often referred to as FRZB, other names also include Fritz, Frzb1, and FRP-3. At the amino acid sequence level, sFRP-3 is highly conserved. The mouse protein shares 76% identity with Xenopus, and 92% with human proteins. The gene for mouse sFRP-3 has been localized to the central region of chromosome 2. Murine sFRP-3 is expressed in the primitive streak during gastrulation, as well as in the retina, foregut diverticulum, nervous system, and posterior mesoderm during development. In adult tissues, sFRP-3 expression, as determined by Northern blot, is detected in the heart, brain, spleen, skeletal muscle, kidney and testis.
The N-terminal portion of sFRP-3 protein shows 50% amino acid identity to the corresponding region of the Drosophila frizzled gene product, a receptor for Wg/Wnt signals. The similarity of sFRP-3 with frizzled proteins is restricted to the N-terminal cysteine-rich domain (CRD) that contains at least ten cysteine residues with highly conserved spacing between them. sFRP-3 was subsequently shown to be a soluble antagonist of Wnt signals. It lacks all transmembrane domains of frizzled proteins but retains the ability to bind Wnts. Ectopic expression of sFRP-3 mRNA has been shown to interfere with the induction of secondary axes in Xenopus embryos injected with Xwnt-8 mRNA.
- Hoang et al. (1996) J. Biol. Chem. 271:26131.
- Leyns et al. (1997) Cell 88:747.
- Wang et al. (1997) Cell 88:757.
- Mayr et al. (1997) Mech. Dev. 63:109.
- Rattner et al. (1997) PNAS 94:2859.
Citations for Recombinant Mouse sFRP-3 Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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HBO Promotes the Differentiation of Neural Stem Cells via Interactions Between the Wnt3/beta-Catenin and BMP2 Signaling Pathways
Authors: C Chen, Y Yang, Y Yao
Cell Transplant, 2019-11-06;0(0):9636897198835.
Species: Rat
Sample Types: Whole Cells
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Wingless-type mammary tumor virus integration site family, member 5A (Wnt5a) regulates human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein 120 (gp120)-induced expression of pro-inflammatory cytokines via the Ca2+/calmodulin-dependent protein kinase II (CaMKII) and c-Jun N-terminal kinase (JNK) signaling pathways.
Authors: Li B, Shi Y, Shu J, Gao J, Wu P, Tang S
J Biol Chem, 2013-03-28;288(19):13610-9.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Secreted frizzled-related protein 3 regulates activity-dependent adult hippocampal neurogenesis.
Authors: Jang M, Bonaguidi M, Kitabatake Y, Sun J, Song J, Kang E, Jun H, Zhong C, Su Y, Guo J, Wang M, Sailor K, Kim J, Gao Y, Christian K, Ming G, Song H
Cell Stem Cell, 2013-02-07;12(2):215-23.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Wnt3a binds to several sFRPs in the nanomolar range.
Authors: Wawrzak D, Metioui M, Willems E, Hendrickx M, de Genst E, Leyns L
Biochem. Biophys. Res. Commun., 2007-04-19;357(4):1119-23.
Species: Mouse
Sample Types: Recombinant Protein, Whole Cells
Applications: Bioassay, Surface Plasmon Resonance -
Frzb modulates Wnt-9a-mediated beta-catenin signaling during avian atrioventricular cardiac cushion development.
Authors: Person AD, Garriock RJ, Krieg PA, Runyan RB, Klewer SE
Dev. Biol., 2005-02-01;278(1):35-48.
Species: Chicken
Sample Types: Whole Tissue
Applications: Bioassay -
Wnt signaling induces the myogenic specification of resident CD45+ adult stem cells during muscle regeneration.
Authors: Polesskaya A, Seale P, Rudnicki MA
Cell, 2003-06-27;113(7):841-52.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
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