Recombinant Mouse Serum Amyloid A2 Protein, CF

Catalog #: 8478-SA
1 Citation Datasheet / COA / SDS

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8478-SA-050

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Recombinant Mouse Serum Amyloid A2 Protein, CF Summary

Product Specifications

Purity

>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to induce TNF-alpha secretion by J774A.1 mouse reticulum cell sarcoma macrophage cells. The ED₅₀ for this effect is 1.5-7.5 μg/mL.

Source

E. coli-derived mouse Serum Amyloid A2 protein
Gly20-Tyr122

Accession #

P05367

N-terminal Sequence
Analysis

Gly20

Predicted Molecular Mass

12 kDa

SDS-PAGE

11 kDa, reducing conditions

Product Datasheets

8478-SA

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8478-SA

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris.
Reconstitution	Reconstitute at 250 μg/mL in sterile water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Serum Amyloid A2

Mouse Serum Amyloid A2 (SAA2) is a multifunctional apolipoprotein produced by hepatocytes in response to pro-inflammatory cytokines (1, 2). It is secreted as a 12 kDa, non-glycosylated protein and circulates as part of the HDL complex (3, 4). The SAA2 gene is one of five SAA genes in mouse (5). SAA2 and SAA1 are categorized as acute phase (A)-SAA proteins based on their structural similarity and inducible expression during chronic inflammation. Mature mouse SAA2 shares 70% amino acid (aa) sequence identity with human SAA2 and 92% sequence identity with mouse SAA1. Secretion of SAA2 is increased during inflammation with amounts of total A-SAA increasing up to 1000-fold (6, 7). Increased levels of A-SAA in serum are indicative of inflammatory disease (8). When highly expressed, SAA can displace ApoA1 as the major apolipoprotein in HDL complexes, weakening the function of HDL as a reverse (lipid clearing) cholesterol transporter (2). A highly charged region of SAA2 and SAA1 (aa 36-68) contains putative fibronectin- and laminin-binding motifs (5). This region also binds heparin sulfate proteoglycans at mildly acidic pH and promotes aggregation of A-SAA (9, 10). Persistent production of A-SAA results in amyloid A amyloidosis, a chronic inflammatory condition that culminates in renal failure (10-12). Amyloidosis is triggered by tissue damage, infection, or other insults that result in prolonged inflammatory cytokine activity. SAA2 selectively accumulates in amyloid fibril deposits during amyloidosis. Circulating levels of total A-SAA decrease during active amyloidosis due to the sequestration of SAA2 into amyloid depositions (13, 14). Mouse strains can differ in SAA sequence, expression and amyloid formation (15).

References

Thorn, C.F. and A.S. Whitehead (2002) Amyloid 9:229.
Benditt, E.P. et al. (1979) Proc. Natl. Acad. Sci. U.S.A. 76:4092.
Hoffman, J.S. et al. (1984) J. Exp. Med. 159:641.
Uhlar, C.M. and A.S. Whitehead (1999) Eur. J. Biochem. 265:501.
van der Westhuyzen, D.R. et al. (2007) Curr. Opin. Lipidol. 18:147.
Hebert, L. and F. Gervais (1990) Scand. J. Immunol. 31:167.
Kluve-Beckerman, B. et al. (1997) Biochem. J. 322(Pt 2):663.
Lu, J. et al. (2014) Proc. Natl. Acad. Sci. U.S.A. 111:5189.
Elimova, E. et al. (2009) FASEB J. 23:3436.
Yu, J. et al. (2000) Lab. Invest. 80:1797.
Jaffe, R.H. (2001) Arch. Pathol. Lab. Med. 125:25.
Yu, J. et al. (2000) Amyloid 7:32.
Meek, R.L. et al. (1986) J. Exp. Med. 163:499.
Simons, J.P. et al. (2013) Proc. Natl. Acad. Sci. U.S.A. 110:16115.
Mori, M. et al. (2014) Exp. Anim. 63:99.

Entrez Gene IDs

6289 (Human); 20209 (Mouse)

Alternate Names

SAA; SAA2; Serum Amyloid A2

Related Research Areas

Citation for Recombinant Mouse Serum Amyloid A2 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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