Recombinant Mouse Reg3A Protein, CF Summary
Product Specifications
Glu27-Gln175, with an N-terminal Met
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
3907-RG
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Reg3A
Reg3A (Regenerating islet‑derived protein 3 alpha), also known as Reg III‑ alpha PAP2 (pancreatitis‑associated protein 2) in rats, or HIP/PAP (human islet peptide/PAP) in humans, is a secreted 16‑17 kDa type III member of the Reg family, which is the group 7 subfamily of C‑type lectins (1, 2). The four type III genes ( alpha -δ) in the mouse Reg family are thought to have cell‑protective and proliferative effects (2‑4). Like other Reg proteins, the 149 amino acid (aa) mature mouse Reg3A (aa 27‑175) contains a small, trypsin‑cleavable PAP domain (aa 27‑39) and a C‑type lectin domain (2, 5). Mature mouse and rat Reg3A share 87% aa sequence identity, while human Reg3A shares 61%, and other mouse type III Regs share less than 60% aa sequence identity with mouse Reg3A. Rodent Reg3A is mainly expressed in the intestinal tract and acinar cells and islet alpha cells of the exocrine pancreas; IL‑6 enhances expression (1, 3, 6, 7). Pancreatic Reg3A expression is also increased in mouse models of type 1 diabetes (4). In the rat brain, nerve injury and inflammation increase Reg3A expression in dorsal root ganglion neurons, while low dopamine levels in stress induce its production by melanocytes (8, 9). Treatment of rat macrophages with Reg3A causes up‑regulation of NFkB signaling and modulates cytokine production (2, 10). In humans, Reg3A expression and proteolytic activation in the small intestine is thought to have a protective effect against infection and TNF‑ alpha ‑induced stress (11).
- Narushima, Y et al. (1997) Gene 185:159.
- Viterbo, D. et al. (2008) J. Immunol. 181:1959.
- Cui, W. et al. (2009) Growth Factors 27:195.
- Lu, Y. et al. (2006) Am. J. Physiol. Endocrinol. Metab. 291:E50.
- Graf, R. et al. (2001) J. Biol. Chem. 276:21028.
- Wang, Y. et al. (2011) Growth Factors 29:72.
- Gurr, W. et al. (2007) Diabetes 56:34.
- He, S.Q. et al. (2010) Mol. Pain 6:23.
- Konishi, H. et al. (2011) Biochem. Biophys. Res. Commun. 407:7.
- Viterbo, D. et al. (2008) J. Immunol. 181:1948.
- Medveczky, P. et al. (2009) Biochem. J. 420:335.
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