Recombinant Mouse Rae-1 beta Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
1198-RA-050
R&D Systems Recombinant Proteins and Enzymes
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Citations (2)
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Recombinant Mouse Rae-1 beta Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the protein to bind immobilized rmNKG2D/Fc Chimera in a functional ELISA.
Source
Mouse myeloma cell line, NS0-derived mouse Rae-1 beta protein
Mouse Rae-1 beta
(Leu29 - Ser231)
Accession # O08603
IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Leu29
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
49.6 kDa (monomer)
SDS-PAGE
70-80 kDa, reducing conditions

Product Datasheets

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1198-RA

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1198-RA

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Rae-1 beta

Rae-1 beta is a member of a family of cell-surface proteins that function as ligands for mouse NKG2D. Other family members are designated Rae-1 alpha, gamma, δ, and epsilon. Amino acid sequence identity within this family ranges from 88 - 95%. The Rae-1 proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding these proteins are not found within the Major Histocompatibility Complex on mouse chromosome 17, but rather map to mouse chromosome 10. The Rae-1 proteins are anchored to the membrane via a GPI-linkage. The name of this family derives from the original identification of these proteins as the product of retinoic acid early inducible transcripts. Rae-1 expression is developmentally controlled. Transcripts were observed in the brain/head region of day 10 - 14 embryos but disappeared by day 18. Rae-1 transcripts were detected in several transformed cell lines but are absent from most normal adult tissues. All Rae-1 family members bind to mouse NKG2D, an activating receptor expressed on NK cells and some T cell subsets, resulting in the activation of cytolytic activity and/or cytokine production by these effector cells. Ectopic expression of Rae-1 on mouse tumor cell lines resulted in the in vivo rejection of the tumors (1 - 6).

References
  1. Zou, Z. et al. (1996) J. Biochem (Tokyo) 119:319.
  2. Diefenbach, A. et al. (2000) Nature Immunol. 1:119.
  3. Cerwenka, A. et al. (2000) Immunity 12:721.
  4. Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521.
  5. Diefenbach, A. et al. (2001) Nature 413:165.
  6. NKG2D and its Ligands, www.RnDSystems.com.
Long Name
Retinoic Acid Early Transcript 1 beta
Entrez Gene IDs
8480 (Human); 19369 (Mouse)
Alternate Names
Rae1 beta; Rae-1 beta

Citations for Recombinant Mouse Rae-1 beta Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. T Cells Engineered With Chimeric Antigen Receptors Targeting NKG2D Ligands Display Lethal Toxicity in Mice.
    Authors: VanSeggelen H, Hammill J, Dvorkin-Gheva A, Tantalo D, Kwiecien J, Denisova G, Rabinovich B, Wan Y, Bramson J
    Mol Ther, 2015-06-30;23(10):1600-10.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Suppressing T cell motility induced by anti-CTLA-4 monotherapy improves antitumor effects.
    J. Clin. Invest., 2012-09-04;122(10):3718-30.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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