Recombinant Mouse NKp46/NCR1 Fc Chimera Protein, CF

Catalog #: 2225-NK
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
2225-NK-050

R&D Systems Recombinant Proteins and Enzymes

1 Image

Product Details

Citations (2)

Supplemental Products

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse NKp46/NCR1 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse NKp46/NCR1 Fc Chimera is immobilized at 0.5 μg/mL (100 μL/well), the concentration of Recombinant Human Vimentin (Catalog # 2105-VI) that produces 50% of the optimal binding response is found to be approximately 30-150 ng/mL.

Source

Mouse myeloma cell line, NS0-derived mouse NKp46/NCR1 protein

Mouse NKp46 (Glu22-Asn255) Accession # Q8C567	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q8C567

N-terminal Sequence
Analysis

Glu22

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

53.1 kDa (monomer)

SDS-PAGE

67-77 kDa, reducing conditions

Product Datasheets

2225-NK

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2225-NK

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: NKp46/NCR1

NKp46, along with NKp30 and NKp44, are activating receptors that have been collectively termed the natural cytotoxicity receptors (NCR) (1). These receptors are expressed almost exclusively by NK cells and play a major role in triggering some of the key lytic activities of NK cells. In human systems, the CD56^dimCD16⁺ subpopulation that makes up the majority of NK cells in the peripheral blood and spleen expresses NKp46 in both resting and activated states (2). The main NK cell population of the lymph node (CD56^brightCD16^-) expresses low levels of NKp46 in resting cells, but expression is up-regulated by IL-2. Mouse NKp46, also known as MAR-1 (3), is a type I transmembrane protein with two extracellular Ig-like domains. It has a positive charge in its transmembrane domain that permits association with the ITAM-bearing signal adapter proteins, CD3 zeta and Fc epsilon RI gamma (4). Studies with neutralizing antibodies indicate that the three NCR are primarily responsible for triggering the NK‑mediated lysis of many human tumor cell lines. Blocking any of the NCRs individually resulted in partial inhibition of tumor cell lysis, but nearly complete inhibition of lysis was observed if all three receptors were blocked simultaneously (5). NKp46 has also been implicated in recognition of virus‑infected cells through its capacity to bind to viral hemagglutinins (6-8).

References

Moretta, L. and A. Moretta (2004) EMBO J. 23:255.
Ferlazzo, G. et al. (2004) J. Immunol. 172:1455.
Biassoni, R. et al. (1999) Eur. J. Immunol. 29:1014.
Westgaard, I. et al. (2004) J. Leukoc. Biol. PMID 15356098.
Pende, D. et al. (1999) J. Exp. Med. 190:1505.
Arnon, T. et al. (2004) Blood 103:664.
Arnon, T. et al. (2001) Eur. J. Immunol. 31:2680.
Mandelboim, O. et al. (2001) Nature 409:1055.

Entrez Gene IDs

9437 (Human); 17086 (Mouse); 100141419 (Porcine); 102115479 (Cynomolgus Monkey)

Alternate Names

CD335 antigen; CD335; hNKp46; Ly94; LY94lymphocyte antigen 94 homolog (activating NK-receptor; NK-p46); Lymphocyte antigen 94 homolog; MAR-1; natural cytotoxicity triggering receptor 1; Natural killer cell p46-related protein; NCR1; NK cell-activating receptor; NKp46; NKP46FLJ99094; NK-p46lymphocyte antigen 94 (mouse) homolog (activating NK-receptor; NK-p46)

Related Research Areas

Citations for Recombinant Mouse NKp46/NCR1 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

The NKï¿½cell receptor NKp46 recognizes ecto-calreticulin on ER-stressed cells
Authors: S Sen Santar, DJ Lee, Â Crespo, JJ Hu, C Walker, X Ma, Y Zhang, S Chowdhury, KF Meza-Sosa, M Lewandrows, H Zhang, M Rowe, A McClelland, H Wu, C Junqueira, J Lieberman
Nature, 2023-04-05;616(7956):348-356.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Viral antigen mediated NKp46 activation of NK cells results in tumor rejection via NK-DC crosstalk.
Authors: Chinnery, Fay, King, Catherin, Elliott, Tim, Bateman, Andrew R, James, Edward
Oncoimmunology, 2012-09-01;1(6):874-883.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay