Recombinant Mouse Neuroglycan C/CSPG5 Protein, CF

Catalog #: 5665-NG
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
5665-NG-050

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Citations (1)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Neuroglycan C/CSPG5 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit Recombinant Human Midkine (Catalog # 258-MD) induced neurite outgrowth in E16-E18 rat embryonic cortical neurons. When 5 x 10⁴ neurons per well are added to a plate pre-incubated with serial dilutions of rmNeuroglycan-C and 1 μg/mL of rhMidkine, the neurite outgrowth promotion is significantly inhibited in a dose dependent manner with an effect at
0.6-1.2 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Neuroglycan C/CSPG5 protein
Val31-Gln420, with a C-terminal 6-His tag

Accession #

AAH55736

N-terminal Sequence
Analysis

Val31

Predicted Molecular Mass

42.1 kDa

SDS-PAGE

85-105 kDa, reducing conditions

Product Datasheets

5665-NG

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5665-NG

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Neuroglycan C/CSPG5

Neuroglycan C (NGC; also CSPG5 and CALEB) is a 120 - 150 kDa type I transmembrane glycoprotein and member of the neuregulin family of proteins (1 - 2). Depending on its expression profile, NGC may be a glycoprotein of 120 kDa, or a chondroitin sulfate (CS) proteoglycan of 150 kDa (2 - 3). Mouse NGC is synthesized as a 566 amino acid (aa) precursor that contains a 30 aa signal sequence, a 393 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 122 aa cytoplasmic region. The ECD contains one CS attachment domain (aa 32 - 273), with CS attachment at Ser117, one EGF-like domain (aa 371 - 413), three potential sites for N-linked glycosylation, and ten potential sites for O-linked glycosylation (4). Splicing variants produce four isoforms for human NGC. Isoform 1 is the standard form. Isoform 2 has a deletion of aa 487 - 513, while isoform 3 has an alternative start site at Met82 and the same deletion. Isoform 4 has a 56 aa substitution for aa 514 - 566. Phosphorylation likely occurs at Ser249, and proteolysis generates a 75 kDa soluble fragment (5). Over aa 31 - 420, mouse NGC shares 84% aa identity with human NGC. NGC is expressed in nervous tissue and is found on retinal ganglion cells, cerebellar Purkinje cells and hippocampal neurons (6). NGC may function as a growth and differentiation factor involved in neuritogenesis. One study shows that the recombinant ectodomain of NGC core protein enhances neurite outgrowth from rat neocortical neurons in culture via phosphatidylinositol 3-kinase and protein kinase C signaling pathways (7). Another study states that NGC is a novel component of midkine receptors, a heparin-binding growth factor that promotes cell attachment and process extension in oligodendroglial precursor-like cells (3). NGC also acts as a growth factor by directly binding ERbB3 tyrosine kinase and transactivating ErbB2 (1).

References

Kinugasa, Y. et al. (2004) Biochem. Biophys Res. Commun 321:1045.
Yasuda, Y. et al. (1998) Neurosci. Res. 32:313.
Ichihara-Tanaka, K. et al. (2006) J. Biol. Chem. 281:30857.
Aono, S. et al. (2004) J. Biol. Chem. 279:46536.
Shuo, T. et al. (2007) J. Neurochem. 102:1561.
Aono, S. et al. (2006) J. Neurosci. Res.83:110.
Nakanishi, K., et al. (2006) J. Biol. Chem. 281:24970.

Entrez Gene IDs

10675 (Human); 29873 (Mouse); 50568 (Rat)

Alternate Names

CALEB; chondroitin sulfate proteoglycan 5 (neuroglycan C); chondroitin sulfate proteoglycan 5; CSPG5; MGC44034; Neuroglycan C; NGC; NGCAcidic leucine-rich EGF-like domain-containing brain protein

Related Research Areas

Citation for Recombinant Mouse Neuroglycan C/CSPG5 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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