Recombinant Mouse Jagged 1 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
10969-JG-050
Recombinant Mouse Jagged 1 Fc Chimera Protein Bioactivity.
2 Images
Product Details
Citations (1)
FAQs
Reviews

Recombinant Mouse Jagged 1 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. Nobta, M. et al. (2005) J. Biol. Chem. 280:15842. The ED50 for this effect is 1.00-6.00 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Jagged 1 protein
Mouse Jagged 1
(Ser32-Ile335)
Accession # Q9QXX0.1
IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Ser32
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
61 kDa
SDS-PAGE
70-78 kDa, reducing conditions.

Product Datasheets

You must select a language.

x

10969-JG

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10969-JG

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity View Larger

Immobilized Recombinant Mouse Jagged 1 Fc Chimera (Catalog # 10969-JG) enhances BMP-2 (355-BM) induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 1.00-6.00 ug/mL.

SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse Jagged 1 Fc Chimera Protein (Catalog # 10969-JG) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 70-78 kDa and 140-160 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Jagged 1

Jagged 1 is a 180 kDa type I transmembrane glycoprotein and member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins (1). Mouse Jagged 1 is synthesized as a precursor protein that contains a 33 amino acid (aa) signal sequence, a 1034 aa extracellular region, a 26 aa transmembrane (TM) segment and a 125 aa cytoplasmic domain. The large extracellular region has a DSL (Delta, Serrate, Lag-2 consensus sequence) domain followed by 16 EGF-like repeats (2). Mouse Jagged 1 shows 98% and 99% aa identity to human and rat Jagged 1 extracellular domains respectively. The extracellular region of Jagged 1 binds to multiple Notch receptors on the cell surface as well as in solid phase binding studies. The DSL motif is necessary for binding to Notch receptors and the EGF repeats modulate the affinity of the interaction with Notch receptors (3). Notch signaling is implicated in many developmental processes in a variety of cell types. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation. Some specific areas where Jagged is involved include hematopoiesis, myogenesis, neurogenesis and development of the vasculature (4). For instance, soluble non-transmembrane forms of Jagged1 influence behavior in fibroblast cells leading to characteristics exhibited by endothelial cells during angiogenesis (5). Soluble Jagged 1 is also capable of maintaining the survival and enhancing the expansion of human stem cells that are capable of reconstituting hematopoietic lineages in vivo (6). Furthermore, Jagged 1 is implicated in human disease: Alagille syndrome, an autosomal dominant disorder characterized by defects in liver, heart, eye, skeletal, craniofacial tissues, and kidney, is caused by mutations in Jagged 1 (7). Depending on cell types and how soluble forms of the ligand are presented, ligand binding can result in activation or inhibition of Notch signaling (8).

References
  1. Ascano, J. M. et al. (2003) J. Biol. Chem. 278:8771.
  2. Lindsell, C.E. et al. (1995) Cell 80:909.
  3. Shimizu, K. et al. (1999) J. Biol. Chem. 274:32961.
  4. Lewis, J. (1998) Stem Cell & Dev. Biol. 9:583.
  5. Small, D. et al. (2001) J. Biol. Chem. 276:32022.
  6. Karanu, F. et al. (2000) J. Exp. Med. 192:1365.
  7. Joutel, A. and E. Tounier-Lasserve (1998) Stem Cell & Dev. Biol. 9:619.
  8. Hicks, C. et al. (2002) J Neurosci. Res. 68:655.
Entrez Gene IDs
182 (Human); 16449 (Mouse); 29146 (Rat)
Alternate Names
AGS; AHDMGC104644; Alagille syndrome; AWS; CD339 antigen; CD339; HJ1; JAG1; Jagged 1; Jagged1; JAGL1; protein jagged-1

Citation for Recombinant Mouse Jagged 1 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Endothelial to mesenchymal Notch signaling regulates skeletal repair
    Authors: Novak, S;Tanigawa, H;Singh, V;Root, SH;Schmidt, TA;Hankenson, KD;Kalajzic, I;
    JCI insight
    Species: Mouse
    Sample Types: In Vivo
    Applications: In vivo assay

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Mouse Jagged 1 Fc Chimera Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Mouse Jagged 1 Fc Chimera Protein, CF and earn rewards!

Have you used Recombinant Mouse Jagged 1 Fc Chimera Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review