Recombinant Mouse IL-6R alpha (aa 20-357) Protein Summary
Product Specifications
Leu20-Glu357, with a C-terminal 10-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1830-SR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
1830-SR/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Scientific Data
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Measured by its binding ability in a full range functional ELISA. Recombinant Mouse IL-6 Protein (406-ML) is immobilized at 2.00 μg/mL (100 μL/well). Catalog # 1830-SR Recombinant Mouse IL-6R alpha (aa 20-357) Protein binds with (306-ML) at an ED50 of 30.0-300 ng/mL.
Reconstitution Calculator
Background: IL-6R alpha
Interleukin 6 (IL-6) is a multifunctional cytokine that exerts its activities by binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa ligand binding subunit (IL-6 R alpha /CD126) and a 130 kDa nonligand-binding signal-transducing subunit (gp130/CD130) (1-4). The mouse IL-6 R alpha cDNA encodes a precursor type I transmembrane protein of 460 amino acids (aa) that contains a 19 aa signal sequence, a 345 aa extracellular ligand binding domain, a 21 aa transmembrane region, and a 75 aa cytoplasmic segment (2). The extracellular segment contains an Ig-like and a fibronectin-type III domain, plus a membrane proximal WSXWS motif. In their extracellular regions, mouse IL-6 R alpha shares 89%, 51% and 50% aa identity with rat, human and porcine IL-6 R alpha, respectively. Unlike gp130 that is expressed ubiquitously, the cellular distribution of IL-6 R alpha is predominantly limited to hepatocytes and leukocyte subpopulations such as monocytes, neutrophils, T and B cells. Soluble IL-6R alpha has been found in various body fluids (5). Two soluble receptor isoforms that arise either from proteolytic cleavage of the membrane-bound IL-6R alpha, or by alternative mRNA splicing (reported only in human) have been described (6, 7). Soluble IL-6 R alpha binds IL-6 with an affinity similar to that of the membrane-bound IL-6 R alpha. More importantly, the soluble IL-6 R alpha /IL-6 complex is capable of interacting with the membrane-bound gp130 to activate cells that lack an integral membrane IL-6 R alpha. It has been documented that elevated soluble IL-6 R is associated with numerous diseases including arthritic lesions, multiple myeloma and Crohn’s disease (6, 7).
- Yamasaki, K. et al. (1988) Science 241:825.
- Sugita, T. et al. (1990) J. Exp. Med. 171:2001.
- Hibi, M. et al. (1990) Cell 63:1149.
- Saito, M. et al. (1992) J. Immunol. 148:4066.
- Novick, D. et al. (1989) J. Exp. Med. 170:1409.
- Jones, S.A. et al. (2001) FASEB J. 15:43.
- Jones, S.A. and S. Rose-John (2002) Biochim. Biophys. Acta 1592:251.
Citations for Recombinant Mouse IL-6R alpha (aa 20-357) Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Maternal and perinatal obesity induce bronchial obstruction and pulmonary hypertension via IL-6-FoxO1-axis in later life
Authors: J Selle, K Dinger, V Jentgen, D Zanetti, J Will, T Georgomano, C Vohlen, R Wilke, B Kojonazaro, O Klymenko, J Mohr, S V Koningsb, CJ Rhodes, A Ulrich, D Hirani, T Nestler, M Odenthal, E Mahabir, S Nayakanti, S Dabral, T Wunderlich, J Priest, W Seeger, J Dötsch, SS Pullamsett, MA Alejandre
Nature Communications, 2022-07-27;13(1):4352.
Species: Human, Mouse
Sample Types: Whole Cells
Applications: Bioassay -
An immune-sympathetic neuron communication axis guides adipose tissue browning in cancer-associated cachexia
Authors: H Xie, C Heier, X Meng, L Bakiri, I Pototschni, Z Tang, S Schauer, VJ Baumgartne, GF Grabner, G Schabbauer, H Wolinski, GR Robertson, G Hoefler, W Zeng, EF Wagner, M Schweiger, R Zechner
Proceedings of the National Academy of Sciences of the United States of America, 2022-03-01;119(9):.
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture -
Blocking interleukin-6 trans-signaling protects against renal fibrosis by suppressing STAT3 activation
Authors: W Chen, H Yuan, W Cao, T Wang, W Chen, H Yu, Y Fu, B Jiang, H Zhou, H Guo, X Zhao
Theranostics, 2019-05-31;9(14):3980-3991.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
The replication and transcription activator of murine gammaherpesvirus 68 cooperatively enhances cytokine-activated, STAT3-mediated gene expression
Authors: HC Foreman, J Armstrong, AL Santana, LT Krug, NC Reich
J. Biol. Chem., 2017-08-15;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Temporal and tissue-specific requirements for T-lymphocyte IL-6 signalling in obesity-associated inflammation and insulin resistance
Authors: E Xu, MMA Pereira, I Karakasili, S Theurich, M Al-Maarri, G Rappl, A Waisman, FT Wunderlich, JC Brüning
Nat Commun, 2017-05-03;8(0):14803.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Progenitor T-cell differentiation from hematopoietic stem cells using Delta-like-4 and VCAM-1
Authors: S Shukla, MA Langley, J Singh, JM Edgar, M Mohtashami, JC Zúñiga-Pfl, PW Zandstra
Nat. Methods, 2017-04-10;14(5):531-538.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Activation of AMP-activated protein kinase rapidly suppresses multiple pro-inflammatory pathways in adipocytes including IL-1 receptor-associated kinase-4 phosphorylation
Authors: Ian P Salt
Mol. Cell. Endocrinol., 2016-11-11;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Temporal expression of growth factors triggered by epiregulin regulates inflammation development.
Authors: Harada M, Kamimura D, Arima Y, Kohsaka H, Nakatsuji Y, Nishida M, Atsumi T, Meng J, Bando H, Singh R, Sabharwal L, Jiang J, Kumai N, Miyasaka N, Sakoda S, Yamauchi-Takihara K, Ogura H, Hirano T, Murakami M
J Immunol, 2015-01-02;194(3):1039-46.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
The soluble interleukin-6 receptor is a mediator of hematopoietic and skeletal actions of parathyroid hormone.
Authors: Cho S, Pirih F, Koh A, Michalski M, Eber M, Ritchie K, Sinder B, Oh S, Al-Dujaili S, Lee J, Kozloff K, Danciu T, Wronski T, McCauley L
J Biol Chem, 2013-01-07;288(10):6814-25.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Cutting edge: Inhibition of IL-6 trans-signaling protects from malaria-induced lethality in mice.
Authors: Wunderlich CM, Delic D, Behnke K, Meryk A, Strohle P, Chaurasia B, Al-Quraishy S, Wunderlich F, Bruning JC, Wunderlich FT
J. Immunol., 2012-03-30;188(9):4141-4.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
CXCL1 can be regulated by IL-6 and promotes granulocyte adhesion to brain capillaries during bacterial toxin exposure and encephalomyelitis.
Authors: Roy M, Richard JF, Dumas A, Vallieres L
J Neuroinflammation, 2012-01-23;9(0):18.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Effect of arachidonic acid on hypoxia-induced IL-6 production in mouse ES cells: Involvement of MAPKs, NF-kappaB, and HIF-1alpha.
Authors: Lee SH, Lee YJ, Han HJ
J. Cell. Physiol., 2010-03-01;222(3):574-85.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Sequential ELISA to profile multiple cytokines from small volumes.
Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG
J. Immunol. Methods, 2005-07-01;302(1):172-81.
Applications: ELISA (Standard)
FAQs
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This protein datasheet indicates I need to use a cross-linking antibody, Catalog # MAB050, for biological activity. What is this antibody and is it really necessary?
The antibody is directed against a 6x histidine repeat and is recommended for use as a cross-linker of proteins with 6x his-tag. Crosslinking is often used for proteins that require receptor trimerization and can result greater biological activity. R&D Systems Quality Control tests the performance of these proteins in the presence of the cross-linking antibody. Therefore, it is necessary to use this antibody when trying to achieve the same level of specific activity described in the datasheet.
Reviews for Recombinant Mouse IL-6R alpha (aa 20-357) Protein
Average Rating: 4.5 (Based on 2 Reviews)
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Reason for Rating: Work as expected with Il-6 together.
