Recombinant Mouse IL-12/IL-23 p40 Monomer Protein, CF

Catalog #: 9807-IL Datasheet / COA / SDS

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9807-IL-050

R&D Systems Recombinant Proteins and Enzymes

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Recombinant Mouse IL-12/IL-23 p40 Monomer Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit IL-23 induced IL-17 secretion by mouse splenocytes. The ED₅₀ for this effect is 3-18 ng/mL.

Source

Mouse myeloma cell line, NS0-derived mouse IL-12/IL-23 p40 protein
Met23-Ser335

Accession #

P43432

N-terminal Sequence
Analysis

Met23

Predicted Molecular Mass

36 kDa

SDS-PAGE

39-57 kDa, reducing conditions

Product Datasheets

9807-IL

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9807-IL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: IL-12/IL-23 p40

Interleukin 12 (IL-12) is the founding member of the IL-12 family of heterodimeric cytokines, which have important immunological functions (1). It is a disulfide-linked, 70 kDa (p70) heterodimeric glycoprotein composed of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. Mouse IL-12p40 is a 40 kDa glycoprotein that shows considerable structural similarity to the extracellular domain of hematopoietin receptors (2). It is synthesized as a 335 amino acid (aa) precursor with a 22 aa signal sequence and a 313 aa mature region that contains a 92 aa fibronectin type III domain and an 84 aa Ig C2-like region and has a high degree of structural homology to type I cytokine receptors. There are two intrachain disulfide bonds and four potential N-linked glycosylation sites (3). Once made, it can exist in multiple forms including monomer, homodimer, heterodimer linked to p19 (forming IL-23), and heterodimer linked to p35 (forming IL-12) (1, 4, 5). Mature mouse IL-12p40 shows 93% and 67% aa sequence identity to rat and human IL-12p40 respectively. The secreted form of the p40 subunit inhibits IL-23 functions and abrogates IL-23-mediated anti-tumor effects (6). Characterization of the IL-12p40 proteins for binding and bioactivity showed that both the p40 monomer and dimer inhibited IL-12 binding to IL-12R (7).

References

Trinchieri, G. et al. (2003) Immunity 19:641.
Egwuagu, C. E. et al. (2015) Cytokine Growth Factor Rev. 26:587.
Tone, Y. et al. (1996) Eur. J. Immunol. 26:1222.
Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.
Oppmann,B. et al. (2000) Immunity 13:715.
Shimozato, O et al. (2005) Immunology 117:22.
Ling, P et al. (1995) J. Immunol. 154:116.

Long Name

Interleukin 12/Interleukin 23 p40

Entrez Gene IDs

3593 (Human); 16160 (Mouse); 64546 (Rat); 397076 (Porcine); 403976 (Canine); 102124858 (Cynomolgus Monkey); 493741 (Feline); 694747 (Rhesus Macaque)

Alternate Names

CLMF p40; CLMF; CLMF2; Cytotoxic lymphocyte maturation factor 40 kDa subunit; IL12 p40; IL-12 p40; IL-12 subunit p40; IL12B; IL-12B; IL-12BNK cell stimulatory factor chain 2; interleukin 12, p40; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocytematuration factor 2, p40); interleukin-12 beta chain; interleukin-12 subunit beta; natural killer cell stimulatory factor, 40 kD subunit; NKSF; NKSF2; NKSF2IL12, subunit p40