Recombinant Mouse IL-12/IL-23 p40 Homodimer Protein

Catalog #: 499-ML
10 Citations Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
499-ML-025/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
499-ML-025

R&D Systems Recombinant Proteins and Enzymes

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Product Details

Citations (10)

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse IL-12/IL-23 p40 Homodimer Protein Summary

Product Specifications

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit the IL-12 dependent proliferation of mouse splenocytes activated with PHA and IL-2. The ED₅₀ for this effect is 1-3 ng/mL in the presence of 0.3 ng/mL of mouse IL-12.

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse IL-12/IL-23 p40 protein
Met23-Ser335

Accession #

P43432.1

N-terminal Sequence
Analysis

Met23

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

35.8 kDa (monomer)

Product Datasheets

499-ML (with carrier)

Product Datasheet

COA

SDS

499-ML/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

499-ML

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

499-ML/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: IL-12/IL-23 p40

Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. IL-12 has multiple effects on T cells and NK cells and is a key mediator in the development of Th1 cells.

IL-12 is a heterodimeric cytokine containing two disulfide-linked subunits, p35 and p40. Human and mouse IL-12 share 70% and 60% amino acid sequence identity in their p40 and p35 subunits, respectively. Although mouse IL-12 is active on human or mouse IL-12 responsive cells, human IL-12 is not active on mouse cells.

The disulfide-linked mouse p40 homodimer can bind to IL-12 receptors and is an antagonist of IL-12 activities in vitro. The mouse p40 monomer is at least ten times less active than the homodimer as an IL-12 antagonist. At the present time, the existence and the physiological role of mouse p40 homodimer in vivo remains to be determined.

Long Name

Interleukin 12/Interleukin 23 p40

Entrez Gene IDs

3593 (Human); 16160 (Mouse); 64546 (Rat); 397076 (Porcine); 403976 (Canine); 102124858 (Cynomolgus Monkey); 493741 (Feline); 694747 (Rhesus Macaque)

Alternate Names

CLMF p40; CLMF; CLMF2; Cytotoxic lymphocyte maturation factor 40 kDa subunit; IL12 p40; IL-12 p40; IL-12 subunit p40; IL12B; IL-12B; IL-12BNK cell stimulatory factor chain 2; interleukin 12, p40; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocytematuration factor 2, p40); interleukin-12 beta chain; interleukin-12 subunit beta; natural killer cell stimulatory factor, 40 kD subunit; NKSF; NKSF2; NKSF2IL12, subunit p40

Related Research Areas

Citations for Recombinant Mouse IL-12/IL-23 p40 Homodimer Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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IL-12 p40 monomer is different from other IL-12 family members to selectively inhibit IL-12R&beta1 internalization and suppress EAE
Authors: S Mondal, M Kundu, M Jana, A Roy, SB Rangasamy, KK Modi, J Wallace, YA Albalawi, R Balabanov, K Pahan
Proc. Natl. Acad. Sci. U.S.A., 2020-08-19;117(35):21557-21567.
Species: Mouse
Sample Types: In Vivo
Applications: Bioassay
Regulation of IL-12p40 by HIF controls Th1/Th17 responses to prevent mucosal inflammation
Authors: E Marks, C Naudin, G Nolan, BJ Goggins, G Burns, SW Mateer, JK Latimore, K Minahan, M Plank, PS Foster, R Callister, M Veysey, MM Walker, NJ Talley, G Radford-Sm, S Keely
Mucosal Immunol, 2017-01-25;0(0):.
Applications: Western Blot
Young microglia restore amyloid plaque clearance of aged microglia
Authors: Anna Daria
EMBO J, 2016-12-21;0(0):.
Species: Mouse
Sample Types: Complex Sample Type
Applications: Bioassay
Free IL-12p40 monomer is a polyfunctional adaptor for generating novel IL-12-like heterodimers extracellularly.
Authors: Abdi K, Singh N, Spooner E, Kessler B, Radaev S, Lantz L, Xiao T, Matzinger P, Sun P, Ploegh H
J Immunol, 2014-05-12;192(12):6028-36.
Species: Mouse
Sample Types:
Applications: Bioassay
Interleukin-12 (IL-12), but not IL-23, induces the expression of IL-7 in microglia and macrophages: implications for multiple sclerosis.
Authors: Jana M, Mondal S, Jana A, Pahan K
Immunology, 2014-04-01;141(4):549-63.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Suppression of regulatory T cells by IL-12p40 homodimer via nitric oxide.
Authors: Brahmachari S, Pahan K
J. Immunol., 2009-07-08;183(3):2045-58.
Species: Mouse
Sample Types: In Vivo, Whole Cells
Applications: Bioassay, In Vivo
Glechoma hederacea inhibits inflammatory mediator release in IFN-gamma and LPS-stimulated mouse peritoneal macrophages.
Authors: An HJ, Jeong HJ, Um JY, Kim HM, Hong SH
J Ethnopharmacol, 2006-03-10;106(3):418-24.
Applications: ELISA (Standard)
17beta-estradiol (E2) modulates cytokine and chemokine expression in human monocyte-derived dendritic cells.
Authors: Bengtsson AK, Ryan EJ, Giordano D, Magaletti DM, Clark EA
Blood, 2004-05-13;104(5):1404-10.
Applications: ELISA (Standard)
A profibrotic function of IL-12p40 in experimental pulmonary fibrosis.
Authors: Huaux F, 107322, Arras M, Tomasi D, Barbarin V, Delos M, Coutelier JP, Vink A, Phan SH, Renauld JC, Lison D
Interferon-gamma modulates articular chondrocyte and osteoblast metabolism through protein kinase R-independent and dependent mechanisms, 2002-09-01;169(5):2653-61.
Species: Mouse
Sample Types: In Vivo, Whole Cells
Applications: Bioassay, In Vivo
AMD3100, a CxCR4 antagonist, attenuates allergic lung inflammation and airway hyperreactivity.
Authors: Lukacs NW, Berlin A, Schols D, Skerlj RT, Bridger GJ
Am. J. Pathol., 2002-04-01;160(4):1353-60.
Applications: ELISA (Standard)