Recombinant Mouse IGF-I R/IGF1R Protein

Catalog #: 6630-GR
3 Citations Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
6630-GR-025/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
6630-GR-025

R&D Systems Recombinant Proteins and Enzymes

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Product Details

Citations (3)

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse IGF-I R/IGF1R Protein Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse (rm) IGF-I R/IGF1R is present at 0.5 μg/mL, the concentration of rmIGF-I that produces 50% of the optimal binding response is found to be approximately 10-60 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived mouse IGF-I R/IGF1R protein Met1-His936 with a C-terminal 6-His tag

Accession #

Q60751

N-terminal Sequence
Analysis

Glu31 & Asp742

Structure / Form

Covalently-linked heterotetramers (2 alpha and 2 beta subunits) & covalently-linked single chain homodimers

Predicted Molecular Mass

104.5 kDa (single chain), 80.7 kDa ( alpha subunit) & 23.2 kDa ( beta subunit)

SDS-PAGE

160-170 kDa, 120-130 kDa & 45-52 kDa, reducing conditions.

Product Datasheets

6630-GR (with carrier)

Product Datasheet

COA

SDS

6630-GR/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6630-GR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	12 months from date of receipt, ≤ -20 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, ≤ -20 degreesC under sterile conditions after reconstitution.

6630-GR/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	12 months from date of receipt, ≤ -20 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, ≤ -20 degreesC under sterile conditions after reconstitution.

Reconstitution Calculator

Background: IGF-I R/IGF1R

The insulin-like growth factor I receptor (IGF-I R or IGF1R, designated CD221) is a 450 kDa disulfide-linked heterotetrameric transmembrane glycoprotein consisting of two 130 kDa alpha and two 95 kDa beta subunits (1, 4). The IGF-1 R cDNA encodes a preproreceptor that is proteolytically cleaved to produce the extracellular alpha subunit (amino acid (aa) 31-737), which contains a cysteine-rich region and two ligand-binding fibronectin type III (FN-III) domains, and the beta subunit (aa 742-1369), which contains an extracellular FN-III domain, transmembrane and cytoplasmic tyrosine kinase domains (1). Extracellular portions of mature mouse IGF-I R share 99.7% aa identity with rat, 96% with human, canine and porcine, and 95% with bovine and equine IGF-I R. IGF-I R is expressed in all cell types and tissues. It binds insulin-like growth factor I (IGF-I) with high affinity, IGF-II with lower affinity, and insulin with lowest affinity (2, 3). Both IGF-I R and the structurally similar insulin receptor (Ins R) activate the same signaling pathways in response to their respective ligands (3). IGF-I R/Ins R hybrids are formed in proportion to their expression and respond primarily to IGF-I, probably down-regulating cellular response to insulin (2, 5). IGF signaling is also modulated by IGF binding proteins and the scavenger receptor, IGF-II R (4). Expression or glycosylation of IGF-I R may be altered in cancer cells (4, 5). Mice lacking IGF-I R show intrauterine growth deficiency and die at birth due to respiratory failure, and IGF-I R mutations in humans can retarded pre- and postnatal growth (6-8). IGF-I and its receptor are particularly important for neurogenesis, with deficiency producing microcephaly and learning disorders (9-11). Low expression of IGF-I R enhances lifespan in both mouse and human by increasing resistance to oxidative stress (12, 13). IGF-I R expression in human embryonic stem cells is important for their survival and clonogenicity (14).

References

Wada, J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:10360.
Slaaby, R. et al. (2006) J. Biol. Sci. 281:25869.
Boucher, J. et al. (2010) J. Biol. Chem. 285:17235.
Samani, A.A. et al. (2007) Endocr. Rev. 28:20.
Chitnis, M.M. et al. (2008) Clin. Cancer Res. 14:6364.
Liu, J-P. et al. (1993) Cell 75:59.
Abuzzahab, M.J. et al. (2003) N. Engl. J. Med. 349:2211.
Walenkamp, M.J.E. and J.M. Wit (2007) Eur. J. Endocrinol. 157:S15.
D’Ercole, A.J. et al. (2008) Endocrinology 149:5958.
Annenkov, A. (2009) Mol. Neurobiol. 40:195.
Mason, J.L. et al. (2003) J. Neurosci. 23:7710.
Holzenberger, M. et al. (2003) Nature 421:182.
Suh, Y. et al. (2008) Proc. Natl. Acad. Sci. USA 105:3438.
Bendall, S.C. et al. (2007) Nature 448:1015.

Long Name

Insulin-like Growth Factor I Receptor

Entrez Gene IDs

3480 (Human)

Alternate Names

CD221 antigen; CD221; EC 2.7.10; EC 2.7.10.1; IGF1R; IGF-1R; IGF-I R; IGF-I receptor; IGFIR; IGF-IR; IGFR; insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; JTK13; MGC142170; MGC142172; MGC18216; soluble IGF1R variant 1; soluble IGF1R variant 2

Related Research Areas

Citations for Recombinant Mouse IGF-I R/IGF1R Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Brain Delivery of IGF1R5, a Single-Domain Antibody Targeting Insulin-like Growth Factor-1 Receptor
Authors: A Yogi, G Hussack, H van Faasse, AS Haqqani, CE Delaney, E Brunette, JK Sandhu, M Hewitt, T Sulea, K Kemmerich, DB Stanimirov
Pharmaceutics, 2022-07-12;14(7):.
Species: Mouse
Sample Types: Recombinant Protein
Applications: SPR
Development and preclinical evaluation of cixutumumab drug conjugates in a model of insulin growth factor receptor I (IGF-1R) positive cancer
Authors: VR Solomon, E Alizadeh, W Bernhard, A Makhlouf, SV Hartimath, W Hill, A El-Sayed, K Barreto, CR Geyer, H Fonge
Sci Rep, 2020-10-29;10(1):18549.
Species: Human
Sample Types: Peptide
Applications: Bioassay
Cancer Vaccines Co-Targeting HER2/Neu and IGF1R
Authors: C De Giovann, L Landuzzi, A Palladini, ML Ianzano, G Nicoletti, F Ruzzi, A Amici, S Croci, P Nanni, PL Lollini
Cancers (Basel), 2019-04-11;11(4):.
Species: Mouse
Sample Types: Serum
Applications: ELISA Capture