Recombinant Mouse IFNAA Protein, CF

Catalog #: 10150-IF Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10150-IF-010
10150-IF-050

Recombinant Mouse IFNAA Protein Bioactivity

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Product Details

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse IFNAA Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681. The ED₅₀ for this effect is <15 pg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived mouse IFNAA protein
Cys24-Glu190

Accession #

Q61718

N-terminal Sequence
Analysis

Cys24

Predicted Molecular Mass

19.3 kDa

SDS-PAGE

16-21 kDa, under reducing conditions

Product Datasheets

10150-IF

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10150-IF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Mouse IFNAA (Catalog # 10150-IF) suppresses viral activity on L-929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. The ED₅₀ for this effect is <15 pg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Mouse IFNAA (Catalog # 10150-IF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 16-21 kDa.

Reconstitution Calculator

Background: IFNAA

Interferon-alpha A (IFNAA), also referred to as interferon-alpha 15 (IFNA15), is one of 14 subtypes within the IFN-alpha family (1). The members of the IFN-alpha family, also known as alpha leukocyte interferons, encompass a group of distinct but closely related proteins which share approximately 80% amino acid (aa) sequence identity and have a similar globular structure composed of five alpha-helices (1-3). IFN-alpha family members signal through a common set of cell surface receptor complex composed of IFNAR2 and IFNAR1 subunits (2). The mature extracellular domain (ECD) of mouse IFNAA is 167 aa and shares 93% and 92% aa sequence identity with mouse and rat, respectively. Most of the members of the murine IFN-alpha family are N-glycosylated, however IFNAA is one of 4 members that lacks a N-glycosylation site (3). IFNAA is one of the many interferon alpha subtypes belonging to the Type I leukocyte IFN family. The Type I IFN family has antiviral, anti-proliferative and natural killer cell activities.

Long Name

Interferon alpha A

Entrez Gene IDs

242517 (Mouse)

Alternate Names

Gm12597; IFNA15; IFNAA