Recombinant Mouse IFN-kappa Protein Summary
Product Specifications
The ED50 for this effect is 0.2-1.2 ng/mL.
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
8437-MK
| Formulation | Lyophilized from a 0.2 μm filtered solution in Sodium Acetate, NaCl and EDTA with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 200 μg/mL in sterile water. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date. |
8437-MK/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Sodium Acetate, NaCl and EDTA. |
| Reconstitution | Reconstitute at 200 μg/mL in sterile water. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date. |
Scientific Data
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Recombinant Mouse IFN-kappa (Catalog # 8437-MK) exhibits anti-viral activity in L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 0.2-1.2 ng/mL.
Reconstitution Calculator
Background: IFN-kappa
Interferon (IFN)-kappa is a member of the type I IFN family, which also includes IFN-alpha, -beta, -epsilon, and -omega. Mouse IFN-kappa is expressed at low levels in peritoneal macrophages and its expression is up-regulated by double-stranded (ds) RNA and IFN-gamma (1). Mice over-expressing IFN-kappa in pancreatic beta cells developed type I diabetes, similar to what has been reported for mice over-expressing IFN-alpha, -beta, and -gamma (1-4). Mouse IFN-kappa shares 68% and 30% amino acid sequence identity with rat and human IFN-kappa, respectively. Human IFN-kappa has been detected in keratinocytes, monocytes, and monocyte-derived dendritic cells and is reported to have contact-dependent antiviral activity (5-7). Human papillomavirus (HPV) 16 oncogene expression, which is necessary for the development of cervical cancer, has been shown to down-regulate human IFN-kappa expression (8-11).
- Vassileva, G. et al. (2003) J. Immunol. 170:5748.
- Stewart, T.A. et al. (1993) Science 260:1942.
- Sarvetnick, N. et al. (1988) Cell 52:773.
- Pelegrin, M. et al. (1998) J. Biol. Chem. 273:12332.
- LaFleur, D.W. et al. (2001) J. Biol. Chem. 276:39765.
- Nardelli, B. et al. (2002) J. Immunol. 169:4822.
- Buontempo, P.J. et al. (2006) J. Interferon Cytokine Res. 26:40.
- Rincon-Orozco, B. et al. (2009) Cancer Res. 69:8718.
- DeCarlo, C.A. et al. (2010) Lab. Invest. 90:1482.
- Reiser, J. et al. (2011) J. Virol. 85:11372.
- Sunthamala, N. et al. (2014) PLoS One 9:e91473.
Citation for Recombinant Mouse IFN-kappa Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Interferon kappa (IFNk) is critical for normal wound repair and is decreased in diabetic wounds
Authors: SJ Wolf, CO Audu, A Joshi, AD denDekker, WJ Melvin, FM Davis, X Xing, R Wasikowski, L Tsoi, S Kunkel, JE Gudjonsson, MX O'Riordan, JM Kahlenberg, KA Gallagher
JCI Insight, 2022-05-09;0(0):.
Species: Mouse
Sample Types:
Applications: Bioassay
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