Recombinant Mouse IFN-alpha/beta R2 Fc Chimera Protein, CF

Catalog #: 1083-AB Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
1083-AB-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse IFN-alpha/beta R2 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse IFN‑ alpha / beta R2 Fc Chimera is immobilized at 2 μg/mL, it binds to Recombinant Mouse IFN-alpha / beta R1 (Catalog # 3039-AB) in the presence of Recombinant Mouse Limitin/IFN-zeta (Catalog # 597-LM). The concentration of rmIFN‑ alpha / beta R1 that produces 50% of the optimal binding response was found to be approximately 1-4 μg/mL.

Source

Mouse myeloma cell line, NS0-derived mouse IFN-alpha/beta R2 protein

Mouse IFN-alpha / beta R2 (Met1-Ala239) with a substitution Lys160Asn Accession # NP_001103968	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

NP_001103968

N-terminal Sequence
Analysis

Ser22

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

51 kDa (monomer)

SDS-PAGE

80-95 kDa, reducing conditions

Product Datasheets

1083-AB

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1083-AB

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: IFN-alpha/beta R2

IFN‑ alpha / beta R2, also known as IFNAR2, is a 100 kDa glycoprotein member of the class II cytokine receptor family. Proteins from this family form heterodimeric receptor complexes that transduce signals from the type I and II interferon, Tissue factor, IL‑10, and IL‑28/IFN‑ lambda families of cytokines (1‑3). IFN‑ alpha / beta R2, in association with IFN‑ alpha / beta R1, is required for mediating the antiviral, antiproliferative, and apoptotic effects of the type I interferons IFN‑ alpha and IFN‑ beta. IFN‑ alpha / beta R2 is the high‑affinity ligand binding subunit of the receptor. Ligand binding is stabilized by the subsequent association with IFN‑ alpha / beta R1, resulting in the formation of a signaling ternary receptor complex (4, 5). Mature mouse IFN‑ alpha / beta R2 consists of a 221 aa extracellular domain (ECD) with two fibronectin type III repeats, a 21 aa transmembrane segment, and a 250 aa cytoplasmic domain. Alternate splicing generates an approximately 45 kDa secreted isoform that corresponds to the ECD (6, 7). Human IFN‑ alpha / beta R2 is also subject to presenilin‑dependent intramembrane proteolysis, resulting in the liberation of nearly the entire ECD as well as the cytoplasmic domain which migrates to the nucleus and can inhibit gene transcription (8). High concentrations of soluble IFN‑ alpha / beta R2 bind and neutralize IFN‑ alpha and IFN‑ beta, while lower concentrations prolong the antiviral activity of circulating IFN‑ beta but not IFN‑ alpha (9, 10). Complexes of the soluble receptor with IFN‑ alpha or IFN‑ beta can also exert agonistic activity in cells that lack IFN‑ alpha / beta R2 (10). Human, but not mouse, IFN‑ alpha / beta R2 constitutively associates with STAT4, which may account for species specific differences observed in type I interferon responses (11). Within the ECD, mouse IFN‑ alpha / beta R2 shares 46% and 67% aa sequence identity with human and rat IFN‑ alpha / beta R2, respectively.

References

Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33.
Pestka, S. et al. (2004) Immunol. Rev. 202:8.
de Weerd, N.A. et al. (2007) J. Biol. Chem. 282:20053.
Lamken, P. et al. (2004) J. Mol. Biol. 341:303.
Arduini, R.M. et al. (1999) Protein Sci. 8:1867.
Owczarek, C.M. et al. (1997) J. Biol. Chem. 272:23865.
Hardy, M.P. et al. (2002) Biochem. J. 356:355.
Saleh, A.Z.M. et al. (2004) Oncogene 23:7076.
McKenna, S.D. et al. (2004) J. Interferon Cytokine Res. 24:119.
Hardy, M.P. et al. (2001) Blood 97:473.
Tyler, D.R. et al. (2007) Mol. Immunol. 44:1864.

Long Name

Interferon alpha/beta Receptor 2

Entrez Gene IDs

3455 (Human); 15976 (Mouse); 102140083 (Cynomolgus Monkey)

Alternate Names

beta receptor10; human interferon alpha; human interferon alpha/beta receptor; IFNABR; IFN-alpha binding protein; IFN-alpha/beta R2; IFN-alpha/beta receptor 2; IFN-alpha-REC; IFNAR2; IFN-aR2; IFNARB; IFNbR2; IFN-bR2; IFN-R; IFN-R-2; interferon (alpha, beta and omega) receptor 2; Interferon alpha binding protein; interferon alpha/beta receptor 2; interferon-alpha/beta receptor beta chain; Type I interferon receptor 2