Recombinant Mouse IFN-alpha 13 Protein, CF

Catalog #: 10481-IF Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10481-IF-010
10481-IF-050

Recombinant Mouse IFN-alpha 13 Protein Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse IFN-alpha 13 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681. The ED₅₀ for this effect is 1-15 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived mouse IFN-alpha 13 protein
Gly23-Glu189

Accession #

Q80SU4.1

N-terminal Sequence
Analysis

Gly23

Predicted Molecular Mass

19 kDa

SDS-PAGE

20-30 kDa, under reducing conditions

Product Datasheets

10481-IF

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10481-IF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Mouse IFN-alpha 13 Protein (10481-IF) suppresses viral activity on L-929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. The ED₅₀ for this effect is 1-15 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Mouse IFN-alpha 13 (Catalog # 10481-IF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 20-30 kDa.

Reconstitution Calculator

Background: IFN-alpha 13

Interferon-alpha 13 (IFN alpha -13), also known as IFN alpha -1/13 and LeIF D, is one of 14 subtypes within the IFN-alpha family (1). Interferons are a family of cytokines with potent antiviral, antiproliferative and immunomodulatory properties, classified based on their binding specificity to cell surface receptors (1). The members of the IFN-alpha family are distinct but closely related proteins sharing approximately 80% amino acid (aa) sequence identity and a globular structure composed of five alpha-helices (1-3). The mature extracellular domain (ECD) of mouse IFN alpha -13 is shares 64% aa sequence identity with human IFN alpha -13. IFN-alpha family members signal through a common set of cell surface receptor complex composed of IFN alpha -R2 and IFN alpha -R1 subunits (2). Individual IFN-alpha subtypes are known to display unique efficacies to viral protection, and IFN alpha -13 displays antiviral activity protective against Theiler's virus, Mengo virus, VSV (4). IFN alpha -13 reproducibly appears to be up-regulated in response to TMEV infection (5). IFN alpha -13 also protects L929 cells against EMCV as tested in our assays.

References