Recombinant Mouse Granzyme G Protein, CF

Recombinant Mouse Granzyme G Protein, CF Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: Granzyme G

Granzyme G is a member of the Granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1). Together with Granzymes D, E and F, it is regulated through pregnancy and by IL-2 and IL-15 in granulated metrial gland cells (2). Human or rat counterpart of mouse Granzyme G has not been found. Like other Granzymes, mouse Granzyme G is not secreted as a zymogen but stored as a fully processed and activated enzyme in the cytoplasmic granules of CTL (3). It is synthesized as a 248 amino acid precursor with a 18 amino acid signal peptide and a 2 amino acid propeptide (3, 4). The mature protein (residues 21‑248) is expressed and purified.

Related Research Areas

• Granzyme-mediated Cell Death
• Group 1 ILCs
• Isolation, Culture, and Identification of Natural Killer Cells
• Natural Killer Cells (NK Cells)
• Serine Proteases and Regulators