Recombinant Mouse FCRL1/FcRH1 Protein, CF Summary
Product Specifications
Met1-Ser219, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
7286-FC
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: FCRL1/FcRH1
Fc Receptor‑Like 1 (FCRL1), also known as FcRH1, IRTA5, and IFGP1, is an approximately 50 kDa protein with sequence homology to classical Fc receptors. The type 1 transmembrane FCRL proteins contain from three to nine immunoglobulin‑like domains. They are differentially expressed within the B cell lineage and can either promote or inhibit B cell proliferation and activation (1, 2). According to R&D Systems testing, FCRL1 binds to purified mouse IgG. Mature mouse FCRL1 consists of a 203 amino acid (aa) extracellular domain (ECD) with two Ig‑like domains, a 21 aa transmembrane segment, and a 103 aa cytoplasmic domain with two immunotyrosine activation motifs (ITAMs) (3, 4). Alternative splicing may generate additional isoforms that lack the transmembrane segment, have a substituted cytoplasmic domain, or essentially consist of the first two Ig‑like domains (4). The ECD of mouse FCRL1 shares 81% aa sequence identity with rat FCRL1 and 62% with comparable regions of human FCRL1 which contains three Ig‑like domains. FCRL1 is expressed on pre‑B cells and naive B cells (5‑7). It is down‑regulated upon B cell activation but up‑regulated on memory B cells (5, 6, 8). FCRL1 is expressed on many B cell lymphoma and leukemia tumor cells with the exception of B cell acute lymphoblastic leukemia (5, 9‑11). Antibody crosslinking of FCRL1 triggers its tyrosine phosphorylation and augments B cell proliferation induced by the B cell receptor (6).
- Ehrhardt, G.R. and M.D. Cooper (2011) Curr. Top. Microbiol. Immunol. 350:89.
- Maltais, L.J. et al. (2006) Nat. Immunol. 7:431.
- Guselnikov, S.V. et al. (2002) Immunogenetics 54:87.
- Davis, R.S. et al. (2004) Int. Immunol. 16:1343.
- Matesanz-Isabel, J. et al. (2011) Immunol. Lett. 134:104.
- Leu, C.-M. et al. (2005) Blood 105:1121.
- Li, F.J. et al. (2008) Blood 112:179.
- Polson, A.G. et al. (2006) Int. Immunol. 18:1363.
- Du, X. et al. (2008) Blood 111:338.
- Kazemi, T. et al. (2008) Int. J. Cancer 123:2113.
- Kazemi, T. et al. (2009) Cancer Immunol. Immunother. 58:989.
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