Recombinant Mouse ErbB3/Her3 Fc Chimera Protein, CF

Catalog #: 4518-RB
2 Citations Datasheet / COA / SDS

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4518-RB-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse ErbB3/Her3 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to bind Recombinant Human NRG1‑ beta 1/HRG1‑ beta 1 Extracellular Domain (Catalog # 377-HB) in a functional ELISA with an estimated K_D <15 nM.

Source

Mouse myeloma cell line, NS0-derived mouse ErbB3/Her3 protein

Mouse ErbB3 (Ser20 - His641) Accession # Q61526	IEGRMDP	Mouse IgG_2A (Glu98 - Lys330)
N-terminus		C-terminus

Accession #

Q61526

N-terminal Sequence
Analysis

Ser20

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

95.6 kDa (monomer)

SDS-PAGE

120-135 kDa, reducing conditions

Product Datasheets

4518-RB

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4518-RB

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: ErbB3/Her3

ErbB3, also called HER3 (human epidermal growth factor receptor 3) in humans, is a type I transmembrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors named for a viral oncogene (1 - 3). ErbB family members serve as receptors for the EGF family of growth factors (1 - 3). Mouse ErbB3 contains a 19 amino acid (aa) signal sequence, a 622 aa extracellular domain (ECD), a 24 aa transmembrane region, and a 677 aa cytoplasmic domain (4). Human ErbB3 has four isoforms created by intron read-through and truncation of the molecule (5). Three of these are secreted and at least one can inhibit ErbB3 activity (6). Little information is available concerning mouse ErbB3 isoforms. The mouse ErbB3 ECD shares 97%, 93%, 92%, 91%, 89% and 88% aa identity with rat, human, bovine, equine, canine and opossum ErbB3, respectively. ErbB3 is found in epithelial cell layers of gastrointestinal, reproductive, urinary, endocrine and nervous systems, skin and muscle (3). Among ErbB family members, only ErbB3 lacks a working kinase domain, requiring heterodimerization with another ErbB receptor for signaling (1 - 3). The heterodimer of ErbB3 with ErbB2, which has no known ligands of its own, is expressed in the majority of breast, skin, ovary and gastrointestinal tumors and transduces a highly mitogenic signal in response to neuregulin 1 (NRG1; heuregulin 1) or NRG2 (3, 7 - 9). These ligands also bind ErbB4 (1). Signaling is aided by the six consensus binding motifs for the SH2 domain and one for the SH3 domain of the regulatory p85 subunit of phosphoinositide 3-kinase (10, 11). Deletion studies in mice demonstrate non-redundant roles for ErbB3 in development of Schwann cells, neural crest cells and heart valves (12, 13). ErbB3, ErbB2 and neuregulin are all required for formation of the sympathetic nervous system (14).

References

Linggi, B. and G. Carpenter (2006) Trends Cell Biol. 16:649.
Citri, A. and Y. Yarden (2006) Nat. Rev. Mol. Cell Biol. 7:505.
Citri, A. et al. (2003) Exp. Cell Res. 284:54.
Swissprot Accession # Q61526.
Lee, H. et al. (1998) Oncogene 16:3243.
Lee, H. et al. (2001) Cancer Res. 61:4467.
Carraway, K.L. 3rd et. al. (1994) J. Biol. Chem. 269:14303.
Sundaresan, S. et al. (1998) Endocrinology 139:4756.
Wallasch, C. et al. (1995) EMBO J. 14:4267.
Hellyer, N.J. et. al. (1998) Biochem. J. 333:757.
Hellyer, N.J. et. al. (2001) J. Biol. Chem. 276:42153.
Riethmacher, D. et al. (1997) Nature 389:725.
Erickson, S.L. et al. (1997) Development 124:4999.
Britsch, S. et al (1998) Genes Dev. 12:1825.

Long Name

Receptor Tyrosine Protein Kinase ErbB3

Entrez Gene IDs

2065 (Human); 13867 (Mouse)

Alternate Names

c-erbB3; EC 2.7.10; EC 2.7.10.1; ErbB3; ErbB-3; erbB3-S; HER3; HER3c-erbB-3; LCCS2; lethal congenital contracture syndrome 2; MDA-BF-1; MGC88033; p180-ErbB3; p45-sErbB3; p85-sErbB3; Proto-oncogene-like protein c-ErbB-3; receptor tyrosine-protein kinase erbB-3; Tyrosine kinase-type cell surface receptor HER3; v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)

Related Research Areas

Citations for Recombinant Mouse ErbB3/Her3 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Inhibition of HER3 activation and tumor growth with a human antibody binding to a conserved epitope formed by domain III and IV
Authors: LC Schmitt, A Rau, O Seifert, J Honer, M Hutt, S Schmid, J Zantow, M Hust, S Dübel, MA Olayioye, RE Kontermann
MAbs, 2017-04-19;0(0):0.
Applications: Bioassay
ErbB2 directly activates the exchange factor Dock7 to promote Schwann cell migration.
Authors: Yamauchi J, Miyamoto Y, Chan JR, Tanoue A
J. Cell Biol., 2008-04-21;181(2):351-65.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay