Recombinant Mouse Ephrin-A2 Fc Chimera Protein, CF

Catalog #: 8415-A2 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
8415-A2-200

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Recombinant Mouse Ephrin-A2 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE with silver staining

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit proliferation of PC‑3 human prostate cancer cells. The ED₅₀ for this effect is 5-30 ng/mL.

Source

Mouse myeloma cell line, NS0-derived mouse Ephrin-A2 protein

Mouse Ephrin-A2 (Glu23-Asn184) Accession # P52801	IEGRMDP	Mouse IgG_2A (Glu98-Lys330)
N-terminus		C-terminus

Accession #

P52801

N-terminal Sequence
Analysis

Glu23

Structure / Form

Disulfied-linked homodimer

Predicted Molecular Mass

46 kDa

SDS-PAGE

52-61 kDa, reducing conditions

Product Datasheets

8415-A2

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8415-A2

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Ephrin-A2

Ephrin-A2, also known as ELF-1, HEK7-L, LERK-6, and EPLG6, is an approximately 20 kDa member of the Ephrin-A family of GPI-anchored ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. Ephrin-A ligands are structurally related to the extracellular domains of the transmembrane Ephrin-B ligands. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. Ephrin-A2 preferentially interacts with receptors in the EphA family (1, 2). Mouse Ephrin-A2 is synthesized with a 20 amino acid (aa) signal peptide, a 164 aa mature chain, and a 25 aa C-terminal propeptide which is removed prior to GPI linkage of Ephrin-A2 to the membrane (3, 4). It shares 93% and 100% aa sequence identity with human and rat Ephrin-A2, respectively. Ephrin-A2 is expressed in discrete regions of the developing nervous system and limb buds (4-7). Its distribution complements the pattern of Eph receptor expression, and this plays an important role in tissue morphogenesis (7-9). Ephrin-A2 exerts an axon repulsive signal which is important for the accurate pathfinding of retinal ganglion cell axons to the tectum and hippocampal axons to the lateral septum (8, 10). Its up-regulation in astrocytes at sites of optic nerve damage may prevent re-innervation by retinal ganglion cells (11). Ephrin-A2 is also expressed on neural progenitor cells in the subventricular zone (SVZ). It interacts with EphA7, triggering reverse signaling through Ephrin-A2 and inhibition of progenitor cell proliferation (9). In the developing limbs, Ephrin-A2 regulates cartilage morphogenesis and the projection of motoneuron axons (6, 7, 12).

References

Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
Shao, H. et al. (1995) J. Biol. Chem. 270:3467.
Cheng, H.-J., and J.G. Flanagan (1994) Cell 79:157.
Kenmuir, C.L. et al. (2012) Anat. Rec. (Hoboken) 295:105.
Ohta, K. et al. (1997) Mech. Dev. 64:127.
Wada, N. et al. (2003) Dev. Biol. 264:550.
Gao, P.-P. et al. (1996) Proc. Natl. Acad. Sci. USA 93:11161.
Holmberg, J. et al. (2005) Genes Dev. 19:462.
Nakamoto, M. et al. (1996) Cell 86:755.
Symonds, A.C.E. et al. (2007) Eur. J. Neurosci. 25:744.
Eberhart, J. et al. (2000) Dev. Neurosci. 22:237.

Entrez Gene IDs

1943 (Human); 13637 (Mouse)

Alternate Names

Cek7-L; EFNA2; ELF-1; EPH-related receptor tyrosine kinase ligand 6; EphrinA2; Ephrin-A2; EPLG6HEK7 ligand; HEK7-L; HEK7-ligand; LERK6; LERK6LERK-6; ligand of eph-related kinase 6