Recombinant Mouse Endorepellin His-tag Protein, CF

Catalog #: 9987-ER Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9987-ER-050

Recombinant Mouse Endorepellin His-tag Protein Bioactivity

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All Endorepellin/Perlecan Proteins and Enzymes

Product Details

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Endorepellin His-tag Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells. The ED₅₀ for this effect is 0.75-4.5 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Endorepellin/Perlecan protein
Glu3683-Ser4383, with a C-terminal 6-His tag

Accession #

NP_032331.2

N-terminal Sequence
Analysis

Glu3683

Predicted Molecular Mass

75.8 kDa

SDS-PAGE

85-95 kDa, reducing conditions

Product Datasheets

9987-ER

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9987-ER

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 300 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	12 months from date of receipt, ≤ -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Immobilized Recombinant Mouse Endorepellin supports the adhesion of SVEC4‑10 mouse vascular endothelial cells. The ED₅₀ for this effect is 0.75-4.5 μg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Mouse Endorepellin/Perlecan was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 85-95 kDa.

Reconstitution Calculator

Background: Endorepellin/Perlecan

Endorepellin is an 80 kDa glycoprotein derived from the C-terminal end of the heparan sulfate proteoglycan Perlecan. Human Perlecan is an approximately 850 kDa basement membrane heparan sulfate proteoglycan containing multiple LDLR, EGF-like, Laminin-like, and immunoglobulin-like domains. Mouse Perlecan lacks several of the EGF, Laminin-like and Ig-like domains found in the human protein (1-3). Mouse Endorepellin, also termed Domain V (DV) of Perlecan, consists of three Laminin G domains separated by a single EGF-like domain. Mouse Endorepellin shares 89% amino acid sequence identity with human Endorepellin. Endorepellin has been shown to broadly inhibit angiogenesis, including endothelial cell migration, collagen-induced capillary morphogenesis, and blood vessel growth (4). Endostatin, an inhibitor of Endorepellin, has been shown to bind Endorepellin directly and block its anti-angiogenic activity (4). Endorepellin can be further processed into a 26 kDa fragment, termed LG3, containing the third Laminin G-like domain of Endorepellin. LG3 possesses anti-angiogenic activity of its own and its release is mediated by either Cathepsin L or BMP-1/Tolloid family cleavage (4-7). Endorepellin binds to Integrin alpha 2/ beta 1, preventing the integrin-dependent adhesion of vascular endothelial cells (EC) to fibronectin and collagen I (7, 8). Additionally, Endorepellin binds to VEGF R1 and VEGF R2 on EC (10). Its binding to VEGF R2 and Integrin alpha 2/beta 1 on EC induces the association and down‑regulation of both proteins, resulting in a reduction in EC migration and overall antiangiogenic activity (4, 7-10). Endorepellin has been shown to disrupt tumor vasculature and inhibit tumor growth, invasion and angiogenesis (8, 11), and it has recently been proposed as a potential stroke therapy by helping with brain repair following injury (12).

References

Whitelock, J.M. et al. (2008) Biochemistry 47:11174.
Murdoch, A.D. et al. (1992) J. Biol. Chem. 267:8544.
Noonan, D.M. et al. (1991) J. Biol. Chem. 266:22939.
Mongiat, M. et al. (2003) J. Biol. Chem. 278:4238.
Cailhier, J.-F. et al. (2008) J. Biol. Chem. 283:27220.
Gonzalez, E.M. et al. (2005) J. Biol. Chem. 280:7080.
Bix, G. et al. (2004) J. Cell Biol. 166:97.
Woodall, B.P. et al. (2008) J. Biol. Chem. 283:2335.
Goyal, A. et al. (2011) J. Biol. Chem. 286:25947.
Nystrom, A. et al. (2009) Blood 114:4897.
Bix, G. et al. (2006) J. Natl. Cancer Inst. 98:1634.
Bix G. (2013) ACS Chem Neurosci. 4:370.

Long Name

Heparan Sulfate Proteoglycan

Entrez Gene IDs

3339 (Human); 15530 (Mouse)

Alternate Names

basement membrane-specific heparan sulfate proteoglycan core protein; endorepellin (domain V region); heparan sulfate proteoglycan 2; Heparan Sulfate Proteoglycan; HSPG; perlecan proteoglycan; perlecan; PLCSchwartz-Jampel syndrome 1 (chondrodystrophic myotonia); PRCAN; SJA; SJS; SJS1