Recombinant Mouse Endoglycan/PODXL2 Protein, CF

Catalog #: 8365-EG Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
8365-EG-050

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Recombinant Mouse Endoglycan/PODXL2 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE with silver staining

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by the ability of the immobilized protein to inhibit the adhesion of CHO Chinese hamster ovary cells transfected with P-Selectin. The ED₅₀ for this effect is 0.1-0.6 μg/mL.

Source

Mouse myeloma cell line, NS0-derived mouse Endoglycan/PODXL2 protein
Gly30-Gly498, with a C-terminal 6-His tag

Accession #

XP_006506309

N-terminal Sequence
Analysis

Gly30

Predicted Molecular Mass

51 kDa

SDS-PAGE

90-124 kDa, reducing conditions

Product Datasheets

8365-EG

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8365-EG

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Endoglycan/PODXL2

Endoglycan, also called Podocalyxin-like 2 protein (PODXL2), is an extensively O-glycosylated type I transmembrane protein in the CD34 family of sialomucins (1, 2). Mature mouse Endoglycan consists of an extracellular domain (ECD) that contains an N-terminal acidic domain characterized by several polyglutamate tracts, a mucin domain, and a membrane-proximal globular domain. Within the ECD, mouse Endoglycan shares 96% and 75% amino acid identity with rat and human Endoglycan, respectively. Following the extracellular domain is a single transmembrane spanning helix and a charged cytoplasmic tail that contains a C-terminal PDZ‑domain binding motif (1, 3). The cytoplasmic domain of Endoglycan has been shown to bind to Na⁺/H⁺ exchanger regulatory factor-1 (NHERF-1), which acts as a scaffolding protein that links membrane proteins to the actin cytoskeleton (4). Endoglycan exists as a disulfide-linked homodimer that is expressed in vascular endothelium, smooth muscle, hematopoietic precursor populations and leukocyte subpopulations (1, 5). Endoglycan functions through its interactions with selectins and is involved in leukocyte-endothelial cell adhesion although, depending on cellular context and post-translational modifications, Endoglycan may also demonstrate anti-adhesive properties (2, 3, 5, 6).

References