Recombinant Mouse DNAM-1/CD226 Fc Chimera Protein, CF

Catalog #: 4436-DN
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
4436-DN-050

Recombinant Mouse DNAM-1/CD226 Fc Chimera Protein Bioactivity

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Citations (1)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse DNAM-1/CD226 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>80%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse Nectin-2/CD112 (Catalog # 3869-N2) is coated at 1 µg/mL, Recombinant Mouse DNAM-1/CD226 Fc Chimera binds with a typical ED₅₀ of 0.3-1.8 μg/mL.

Source

Mouse myeloma cell line, NS0-derived mouse DNAM-1/CD226 protein

Mouse DNAM-1/CD226 (Glu19-Pro254) Accession # Q8K4F0	IEGRMDP	Mouse IgG_2A (Glu98-Lys330)
N-terminus		C-terminus

Accession #

Q8K4F0

N-terminal Sequence
Analysis

Glu19

Predicted Molecular Mass

53.9 kDa

SDS-PAGE

70-90 kDa, reducing conditions

Product Datasheets

4436-DN

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4436-DN

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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When Recombinant Mouse Nectin-2/ CD112 (Catalog # 3869-N2) is coated at 1 μg/mL, Recombinant Mouse DNAM-1/CD226 Fc Chimera (Catalog # 4436-DN) binds with a typical ED₅₀ of 0.3-1.8 μg/mL.

Reconstitution Calculator

Background: DNAM-1/CD226

DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (1). Mature mouse DNAM has a 236 amino acid (aa) extracellular domain (ECD) that contains two Ig-like C2-set domains, and possesses a 58 aa cytoplasmic region that contains motifs for binding PDZ domains and band 4.1 family proteins (1, 2). Within the ECD, mouse DNAM-1 shares 52% and 86% aa sequence identity with human and rat DNAM-1, respectively. Additional cDNA transcripts of mouse DNAM-1 may give rise to secreted or transmembrane isoforms with ECD deletions. DNAM-1 is expressed on several lymphoid and myeloid cell types and interacts with CD155/PVR and Nectin-2/CD112 (2-4). Ligation of DNAM-1 promotes the activation of NK cells, CD8⁺ T cells, and mast cells (3-7), induces dendritic cell maturation, initiates megakaryocyte and activated platelet adhesion to vascular endothelial cells, and stimulates monocyte extravasation; conversely, it inhibits the formation of osteoclasts (8-11). Platelet-endothelium interactions that are mediated by DNAM-1 enable the metastasis of tumor cells to the lung (12). On activated, but not resting NK, T, and mast cells, the cis association of DNAM-1 with CD18 contributes to tyrosine and serine phosphorylation of DNAM-1 during activation (7, 10, 13-15).

References

Fuchs, A. and M. Colonna (2006) Semin. Cancer Biol. 16:359.
Tahara-Hanaoka, S. et al. (2005) Biochem. Biophys. Res. Commun. 329:996.
Bottino, C. et al. (2003) J. Exp. Med. 198:557.
Tahara-Hanaoka, S. et al. (2004) Int. Immunol. 16:533.
Shibuya, A. et al. (1996) Immunity 4:573.
Dardalhon, V. et al. (2005) J. Immunol. 175:1558.
Bachelet, I. et al. (2006) J. Biol. Chem. 281:27190.
Reymond, N. et al. (2004) J. Exp. Med. 199:1331.
Kakehi, S. et al. (2007) Mol. Cell. Biochem. 301:209.
Kojima, H. et al. (2003) J. Biol. Chem. 278:36748.
Tahara-Hanaoka, S. et al. (2006) Blood 107:1491.
Morimoto, K. et al. (2007) Oncogene July 16 [Epub ahead of print].
Shibuya, K. et al. (1999) Immunity 11:615.
Shibuya, K. et al. (2003) J. Exp. Med. 198:1829.
Shibuya, A. et al. (1998) J. Immunol. 166:1671.

Long Name

DNAX Accessory Molecule 1

Entrez Gene IDs

10666 (Human); 225825 (Mouse); 307199 (Rat); 102117316 (Cynomolgus Monkey)

Alternate Names

CD226 antigenplatelet and T cell activation antigen 1; CD226 molecule; CD226; DNAM1; DNAM-1; DNAM1adhesion glycoprotein; DNAM-1DNAX accessory molecule-1; DNAX accessory molecule 1; PTA1; T lineage-specific activation antigen 1 antigen; TLiSA1

Related Research Areas

Citation for Recombinant Mouse DNAM-1/CD226 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Essential function for SAP family adaptors in the surveillance of hematopoietic cells by natural killer cells.
Authors: Dong Z, Cruz-Munoz ME, Zhong MC, Chen R, Latour S, Veillette A
Nat. Immunol., 2009-08-02;10(9):973-80.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry