Recombinant Mouse Dectin-2/CLEC6A Protein, CF

Catalog #: 1525-DC
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
1525-DC-050

R&D Systems Recombinant Proteins and Enzymes

1 Image

Product Details

Citations (2)

Supplemental Products

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Dectin-2/CLEC6A Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to bind biotinylated alpha -D-Mannose-PAA in a functional ELISA.

Source

Mouse myeloma cell line, NS0-derived mouse Dectin-2/CLEC6A protein
Phe43-Leu209, with an N-terminal 7-His tag and Ala

Accession #

Q9JKF4.1

N-terminal Sequence
Analysis

His

Predicted Molecular Mass

20.6 kDa

SDS-PAGE

24 kDa, reducing conditions

Product Datasheets

1525-DC

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1525-DC

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Dectin-2/CLEC6A

Dectin-2 alpha, also known as CLEC6A, CLECSF10, and NKCL, belongs to the C-type lectin family of transmembrane immune regulatory glycoproteins. Dectin-2 alpha, CLEC4A/DCIR, CLEC4B/DCAR, CLEC4C/DLEC, CLEC4D/MCL, and CLEC4E/mincle constitute a subgroup of these molecules that exhibit approximately 40% amino acid (aa) sequence identity in their extracellular domains (ECD) and have a conserved cysteine spacing in their carbohydrate recognition domains (CRD) (1, 2). Mature mouse Dectin-2 alpha consists of a short cytoplasmic tail, a transmembrane segment, and an ECD with a stalk region and one CRD (3, 4). Alternate splicing leads to partial deletion of the transmembrane segment and stalk ( beta isoform) or a portion of the CRD ( gamma isoform) (4). The full length Dectin-2 alpha isoform is a 27 kDa molecule that is primarily expressed on the surface of tissue macrophages and their precursors (3 - 6). The CRD of Dectin-2 alpha contains an EPN motif which is characteristic of calcium-dependent mannose-binding lectins. Dectin-2 alpha selectively interacts with high mannose structures in the Man₉GlcNAc₂ configuration (7). It mediates the recognition of a variety of microorganisms, particularly the filamentous forms of yeast and fungii (7, 8). The short cytoplasmic tail does not contain signaling motifs but mediates association with the ITAM-containing Fc receptor gamma subunit in macrophages (8). Ligation of Dectin-2 alpha induces tyrosine phosphorylation of the gamma subunit, activation of NF kappa B, and enhanced release of TNF-alpha and IL-1ra (8). Macrophage Dectin-2 alpha is upregulated in vivo by inflammatory stimuli and UV-B irradiation (6). It mediates the breaking of UV-induced tolerance by interacting with CD4⁺CD25⁺ regulatory T cells which then induce dendritic cells to release IL-4, IL-10, and TGF-beta (9). Within the ECD, mouse Dectin-2 alpha shares 71% aa sequence identity with human and bovine Dectin-2.

References

Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
Kanazawa, N. et al. (2004) Immunobiology 209:179.
Fernandes, M.J. et al. (1999) Cancer Res. 59:2709.
Ariizumi, K. et al. (2000) J. Biol. Chem. 275:11957.
Bonkobara, M. et al. (2005) Photochem. Photobiol. 81:944.
Taylor, P.R. et al. (2005) Eur. J. Immunol. 35:2163.
McGreal, E.P. et al. (2006) Glycobiology 16:422.
Sato, K. et al. (2006) J. Biol. Chem. 281:38854.
Aragane, Y. et al. (2003) J. Immunol. 171:3801.

Entrez Gene IDs

93978 (Human); 56620 (Mouse)

Alternate Names

CLEC4N; CLEC6A; CLECSF10; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 10; C-type lectin domain family 6 member A; C-type lectin domain family 6, member A; C-type lectin superfamily member 10; DC-associated C-type lectin 2; dectin 2; Dectin2; Dectin-2; Dendritic cell-associated C-type lectin 2

Related Research Areas

Citations for Recombinant Mouse Dectin-2/CLEC6A Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

The ligation between ERMAP, galectin-9 and dectin-2 promotes Kupffer cell phagocytosis and antitumor immunity
Authors: Li, J;Liu, XG;Ge, RL;Yin, YP;Liu, YD;Lu, WP;Huang, M;He, XY;Wang, J;Cai, G;Sun, SH;Yuan, JH;
Nature immunology
Species: N/A
Sample Types: Protein, Recombinant Protein
Applications: ELISA Capture, Immunoprecipitation
Molecular basis for intestinal mucin recognition by galectin-3 and C-type lectins
Authors: C Leclaire, K Lecointe, PA Gunning, S Tribolo, DW Kavanaugh, A Wittmann, D Latousakis, DA MacKenzie, N Kawasaki, N Juge
FASEB J., 2018-01-29;0(0):fj201700619R.
Species: Mouse
Sample Types: Recombinant Protein
Applications: Bioassay, Mass Spectrometry