Recombinant Mouse CXCL17/VCC-1 Protein

Name: Recombinant Mouse CXCL17/VCC-1 Protein
Brand: R&D Systems
SKU: 4270-DM
Rating: 5 (1 reviews)

Catalog #: 4270-DM
3 Citations 1 Review Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
4270-DM-025/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
4270-DM-025

R&D Systems Recombinant Proteins and Enzymes

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All CXCL17/VCC-1 Proteins and Enzymes

Product Details

Citations (3)

FAQs

Reviews (1)

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse CXCL17/VCC-1 Protein Summary

Product Specifications

Purity

>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to induce VEGF expression in mouse endothelial cells. Weinstein, E.J. et al. (2006) BBRC 350:74. The ED₅₀ for this effect is 1-5 µg/mL.

Source

E. coli-derived mouse CXCL17/VCC-1 protein
Ser23-Leu119

Accession #

Q5UW37.1

N-terminal Sequence
Analysis

Ser31

Predicted Molecular Mass

10.3 kDa

Product Datasheets

4270-DM (with carrier)

Product Datasheet

COA

SDS

4270-DM/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4270-DM

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

4270-DM/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: CXCL17/VCC-1

Dendritic cell and monocyte chemokine-like protein (DMC), also known as VEGF-correlated chemokine-1 (VCC-1), is a secreted molecule with a size and predicted three-dimensional folding pattern similar to that of chemokines CXCL8/IL-8 and CXCL14/BRAK (1, 2). It has no predicted N-glycosylation sites, so cleavage of a 22 amino acid (aa) signal sequence likely results in a mature mouse DMC molecule of 97 aa and 11 kDa size. DMC is constitutively produced by airway and intestinal epithelium (1). It induces the chemotaxis of quiescent, but not LPS‑activated peripheral blood monocytes and dendritic cells, and also binds these cells specifically (1). DMC expression is increased in endothelial cells when they are induced to form tubes in vitro (2). Transgenic overexpression in NIH3T3 cells causes up‑regulation of proteins such as VEGF and FGF basic, and increases cell growth rate and tumorigenicity (2). DMC and two other chemokines that play roles in angiogenesis, CXCL1/GRO and CXCL8/IL-8, show significantly correlated expression with that of VEGF in primary lung, breast and esophageal tumors (2). DMC is therefore suggested to play a role in tumor angiogenesis. Mature mouse DMC shares 82%, 71% and 66% amino acid sequence identity with rat, human and bovine DMC, respectively.

References

Pisabarro, M. T. et al. (2006) J. Immunol. 176:2069.
Weinstein, E. J. et al. (2006) Biochem. Biophys. Res. Commun. 350:74.

Entrez Gene IDs

284340 (Human); 232983 (Mouse)

Alternate Names

Chemokine (C-X-C Motif) Ligand 17; C-X-C Motif Chemokine 17; CXCL17; Dcip1; Dendritic Cell And Monocyte Chemokine-Like Protein; DMC; UNQ473; VCC1; VCC-1; VEGF Coregulated Chemokine 1; VEGF Co-Regulated Chemokine 1

Related Research Areas

Citations for Recombinant Mouse CXCL17/VCC-1 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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CXCL17-mediated downregulation of type I collagen via MMP1 and miR-29 in skin fibroblasts possibly contributes to the fibrosis in systemic sclerosis
Authors: S Shimada, K Makino, M Jinnin, S Sawamura, Y Kawano, M Ide, I Kajihara, T Makino, S Fukushima, H Ihn
J Dermatol Sci, 2020-09-29;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: Bioassay
CXCL17-derived CD11b+Gr-1+ myeloid-derived suppressor cells contribute to lung metastasis of breast cancer through platelet-derived growth factor-BB
Authors: YL Hsu, MC Yen, WA Chang, PH Tsai, YC Pan, SH Liao, PL Kuo
Breast Cancer Res., 2019-02-12;21(1):23.
Species: Mouse
Sample Types: In Vivo, Whole Cells
Applications: Bioassay, In Vivo
CXCL17 is a major chemotactic factor for lung macrophages.
Authors: Burkhardt A, Maravillas-Montero J, Carnevale C, Vilches-Cisneros N, Flores J, Hevezi P, Zlotnik A
J Immunol, 2014-06-27;193(3):1468-74.
Species: Mouse
Sample Types: In Vivo, Whole Cells
Applications: Bioassay