Recombinant Mouse CXCL13/BLC/BCA-1 Protein

Catalog #: 470-BC
19 Citations Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
470-BC-025/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
470-BC-025

R&D Systems Recombinant Proteins and Enzymes

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All CXCL13/BLC/BCA-1 Proteins and Enzymes

Product Details

Citations (19)

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse CXCL13/BLC/BCA-1 Protein Summary

Product Specifications

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR5. The ED₅₀ for this effect is 0.5‑2 µg/mL.

Source

E. coli-derived mouse CXCL13/BLC/BCA-1 protein
Ile22-Ala109

Accession #

Q3U1E8

N-terminal Sequence
Analysis

Ile22

Predicted Molecular Mass

9.8 kDa

Product Datasheets

470-BC (with carrier)

Product Datasheet

COA

SDS

470-BC/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

470-BC

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution	Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

470-BC/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: CXCL13/BLC/BCA-1

CXCL13, also known as B-lymphocyte chemoattractant (BLC), is a CXC chemokine that is constitutively expressed in secondary lymphoid organs. Mouse BCA-1 cDNA encodes a precursor protein of 109 amino acid residues with a putative leader sequence of 21 residues. Mature mouse BCA-1 shares 64% amino acid sequence similarity with the human protein and 23 - 34% amino acid sequence identity with other known CXC chemokines. Recombinant or chemically synthesized BCA-1 is a potent chemoattractant for B lymphocytes but not T lymphocytes, monocytes or neutrophils. BLR1, a G protein-coupled receptor originally isolated from Burkitt’s lymphoma cells, has now been shown to be the specific receptor for BCA-1. Among cells of the hematopoietic lineages, the expression of BLR1, now designated CXCR5, is restricted to B lymphocytes and a subpopulation of T helper memory cells. Mice lacking BLR1 have been shown to lack inguinal lymph nodes. These mice were also found to have impaired development of Peyer’s patches and defective formation of primary follicles and germinal centers in the spleen as a result of the inability of B lymphocytes to migrate into B cell areas.

References

Gunn, M.D. et al. (1998) Nature, 391:799.
Legler, D.F. et al. (1998) J. Exp. Med. 187:655.
Forster, R. et al. (1996) Cell 87:1037.

Entrez Gene IDs

10563 (Human); 55985 (Mouse)

Alternate Names

ANGIE; ANGIE2; B cell-attracting chemokine 1; B lymphocyte chemoattractant; BCA1; BCA-1; BCA1B-cell chemoattractant; BCA-1CXC chemokine BLC; B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1); BLC; BLCSmall-inducible cytokine B13; BLR1L; B-lymphocyte chemoattractant; chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant); chemokine (C-X-C motif) ligand 13; C-X-C motif chemokine 13; CXCL13; SCYB13; SCYB13B-cell-attracting chemokine 1; small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cellchemoattractant)

Related Research Areas

Citations for Recombinant Mouse CXCL13/BLC/BCA-1 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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CXCL13 contributes to chronic pain of a mouse model of CRPS-I via CXCR5-mediated NF-?B activation and pro-inflammatory cytokine production in spinal cord dorsal horn
Authors: Wang, J;Yin, C;Pan, Y;Yang, Y;Li, W;Ni, H;Liu, B;Nie, H;Xu, R;Wei, H;Zhang, Y;Li, Y;Hu, Q;Tai, Y;Shao, X;Fang, J;Liu, B;
Journal of neuroinflammation
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
CXCL13-producing CD4+ T cells accumulate in early phase of tertiary lymphoid structures in ovarian cancer
Authors: M Ukita, J Hamanishi, H Yoshitomi, K Yamanoi, S Takamatsu, A Ueda, H Suzuki, Y Hosoe, Y Furutake, M Taki, K Abiko, K Yamaguchi, H Nakai, T Baba, N Matsumura, A Yoshizawa, H Ueno, M Mandai
JCI Insight, 2022-06-22;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
Kindlin-3 maintains marginal zone B cells but confines follicular B cell activation and differentiation
Authors: A Härzschel, L Li, PW Krenn, E Szenes-Nag, G Andrieux, E Bayer, D Pfeifer, L Polcik, U Denk, JP Höpner, E Karabatak, DJ Danner, S Tangermann, L Kenner, H Jumaa, R Greil, M Börries, R Ruppert, PC Maity, TN Hartmann
Journal of leukocyte biology, 2021-12-10;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Group 3 innate lymphoid cells mediate early protective immunity against tuberculosis
Authors: A Ardain, R Domingo-Go, S Das, SW Kazer, NC Howard, A Singh, M Ahmed, S Nhamoyebon, J Rangel-Mor, P Ogongo, L Lu, D Ramsuran, M de la Luz, T K Ulland, M Darby, E Park, F Karim, L Melocchi, R Madansein, KJ Dullabh, M Dunlap, N Marin-Agud, T Ebihara, T Ndung'u, D Kaushal, AS Pym, JK Kolls, A Steyn, J Zúñiga, W Horsnell, WM Yokoyama, AK Shalek, HN Kløverpris, M Colonna, A Leslie, SA Khader
Nature, 2019-06-05;570(7762):528-532.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Impaired lymphocyte trafficking in mice deficient in the kinase activity of PKN1
Authors: R Mashud, A Nomachi, A Hayakawa, K Kubouchi, S Danno, T Hirata, K Matsuo, T Nakayama, R Satoh, R Sugiura, M Abe, K Sakimura, S Wakana, H Ohsaki, S Kamoshida, H Mukai
Sci Rep, 2017-08-09;7(1):7663.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
The Small Rho GTPase TC10 Modulates B Cell Immune Responses
Authors: M Burbage, SJ Keppler, B Montaner, PK Mattila, FD Batista
J. Immunol., 2017-07-26;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Protein Kinase C Epsilon Cooperates with PTEN Loss for Prostate Tumorigenesis through the CXCL13-CXCR5 Pathway
Authors: R Garg, JM Blando, CJ Perez, MC Abba, F Benavides, MG Kazanietz
Cell Rep, 2017-04-11;19(2):375-388.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Efficient Plasma Cell Differentiation and Trafficking Require Cxcr4 Desensitization
Cell Rep, 2016-09-27;17(1):193-205.
Species: Mouse
Sample Types:
Applications: Bioassay
IL-10+ Innate-like B Cells Are Part of the Skin Immune System and Require ?4?1 Integrin To Migrate between the Peritoneum and Inflamed Skin
Authors: SA Geherin, D Gómez, RA Glabman, G Ruthel, A Hamann, GF Debes
J. Immunol, 2016-02-05;196(6):2514-25.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Infection of Female BWF1 Lupus Mice with Malaria Parasite Attenuates B Cell Autoreactivity by Modulating the CXCL12/CXCR4 Axis and Its Downstream Signals PI3K/AKT, NFkappaB and ERK.
Authors: Badr G, Sayed A, Abdel-Maksoud M, Mohamed A, El-Amir A, Abdel-Ghaffar F, Al-Quraishy S, Mahmoud M
PLoS ONE, 2015-04-24;10(4):e0125340.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

An essential role for RGS protein/Galphai2 interactions in B lymphocyte-directed cell migration and trafficking.
Authors: Hwang I, Park C, Harrison K, Boularan C, Gales C, Kehrl J
J Immunol, 2015-01-23;194(5):2128-39.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

A novel CMKLR1 small molecule antagonist suppresses CNS autoimmune inflammatory disease.
Authors: Graham K, Zhang J, Lewen S, Burke T, Dang T, Zoudilova M, Sobel R, Butcher E, Zabel B
PLoS ONE, 2014-12-01;9(12):e112925.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Control of lymphocyte egress from lymph nodes through beta2-adrenergic receptors.
Authors: Nakai A, Hayano Y, Furuta F, Noda M, Suzuki K
J Exp Med, 2014-11-24;211(13):2583-98.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Sphingosine-1-phosphate receptor 2 is critical for follicular helper T cell retention in germinal centers.
Authors: Moriyama S, Takahashi N, Green J, Hori S, Kubo M, Cyster J, Okada T
J Exp Med, 2014-06-09;211(7):1297-305.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

MIF promotes B cell chemotaxis through the receptors CXCR4 and CD74 and ZAP-70 signaling.
Authors: Klasen C, Ohl K, Sternkopf M, Shachar I, Schmitz C, Heussen N, Hobeika E, Levit-Zerdoun E, Tenbrock K, Reth M, Bernhagen J, El Bounkari O
J Immunol, 2014-04-23;192(11):5273-84.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Follicular regulatory T cells control humoral autoimmunity via NFAT2-regulated CXCR5 expression.
Authors: Vaeth M, Muller G, Stauss D, Dietz L, Klein-Hessling S, Serfling E, Lipp M, Berberich I, Berberich-Siebelt F
J Exp Med, 2014-03-03;211(3):545-61.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Rgs13 constrains early B cell responses and limits germinal center sizes.
Authors: Hwang I, Hwang K, Park C, Harrison K, Kehrl J
PLoS ONE, 2013-03-22;8(3):e60139.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Factors expressed by murine embryonic pancreatic mesenchyme enhance generation of insulin-producing cells from hESCs.
Authors: Guo T, Landsman L, Li N, Hebrok M
Diabetes, 2013-01-10;62(5):1581-92.
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

The inflammasome adaptor ASC regulates the function of adaptive immune cells by controlling Dock2-mediated Rac activation and actin polymerization.
Authors: Ippagunta SK, Malireddi RK, Shaw PJ
Nat. Immunol., 2011-09-04;12(10):1010-6.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay