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Recombinant Mouse Cripto Protein

Catalog #: 1538-CR
6 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
1538-CR-010/CF

With Carrier

Catalog # Availability Size / Price Qty
1538-CR-010
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (6)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Cripto Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized rmActivin RIB/Fc Chimera at 1 µg/mL (100 µL/well) can bind rmCripto with a linear range of 0.8-100 ng/mL. This protein will also bind rmNodal in a functional ELISA.
Source
Mouse myeloma cell line, NS0-derived mouse Cripto protein
 
Mouse Cripto
(Arg26-Gln150)
Accession # P51865		 DI 6-His tag
N-terminus C-terminus
Accession #
P51865
N-terminal Sequence
Analysis
Arg26
Predicted Molecular Mass
15 kDa
SDS-PAGE
20-25 kDa, reducing conditions

Product Datasheets

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1538-CR (with carrier)

Product Datasheet
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1538-CR/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1538-CR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1538-CR/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cripto

Cripto is the founding member of the epidermal growth factor-CriptoFRL1Cryptic (EGF-CFC) family of signaling proteins that function in cancer and various developmental processes. These developmental processes include: formation of the germ layers and dorsal organizer, specification of anterior-posterior and left-right axes, and differentiation of heart muscle (1, 2). Other members of the EGF-CFC family include Cryptic, Xenopus FRL-1 and zebrafish OEP (one-eyed pinhead). Overall sequence identity between members of the family is low, but they do share several common domains: a variant EGF-like motif, a novel conserved cysteine-rich domain (called CFC domain), and a C-terminal hydrophobic region. Most EGF-CFC members have a glycosyl-phosphatidylinositol (GPI) anchoring site at the C-terminus and exist as extracellular membrane-anchored proteins. However, naturally-occurring soluble isoforms also exist. Mouse Cripto shares 66% and 34% amino acid identity with human Cripto and zebrafish OEP, respectively (2). Despite weak conservation in amino acid identity, EGF-CFC family members appear to function similarly in assays for phenotypic rescue of zebrafish oep mutants (2). Both secreted and membrane bound forms of Cripto demonstrate biological activity (3).

Cripto, also known as CFC-2 or TDGF-1 (teratocarcinoma-derived growth factor), was originally isolated from an undifferentiated human teratocarcinoma cell line as a potential oncogene. It is overexpressed in many types of cancers and acts as a growth factor for tumors (4). Genetic evidence from mice and zebrafish points to a role for Cripto as an essential cofactor in Nodal signaling. Cripto and OEP mutants display defects in mesoderm induction and heart morphogenesis, similar to phenotypes seen in Nodal mutants (2).

Cripto acts as a cofactor for Nodal by recruiting the Activin type I Receptor, ALK-4, leading to an Act RIIB-ALK4-Cripto-Nodal complex for signaling (1, 3). Cripto also forms a complex with activin and Act RIIs to block activin signaling (5). Work has shown that other TGF-beta superfamily members such as Vg1 and GDF-1 also require EGF-CFC cofactors (6). Cripto can also activate mitogen-activated protein kinase (MAPK) and Akt pathways independently of Nodal by directly binding to a membrane-associated heparan sulfate proteoglycan, glypican-1 (7).

References
  1. Rosa, F.M. (2002) Science’s STKE http://stke.sciencemag.org/.
  2. Shen, M. and A. Schier (2000) Trends Genet. 16:303.
  3. Yan, Y-T. et al. (2002) Mol. Cell Biol. 22:4439.
  4. Salomon, D. et al. (2000) Endocrine-Rel. Cancer 7:199.
  5. Gray, P.C. et al. (2003) Proc. Natl. Acad. Sci. USA 100:5193.
  6. Cheng, S. et al. (2003) Genes & Dev. 17:31.
  7. Bianco, C. et al. (2003) Cancer Research 63:1192.
Long Name
Teratocarcinoma-derived Growth Factor/Cripto Growth Factor
Entrez Gene IDs
6997 (Human); 21667 (Mouse)
Alternate Names
CFC-2; CR; Cripto; Cripto-1 growth factor; Cripto-1; CRIPTOCRGF; Epidermal growth factor-like cripto protein CR1; TDGF1; TDGF-1; teratocarcinoma-derived growth factor 1

Citations for Recombinant Mouse Cripto Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. CRIPTO antagonist ALK4L75A-Fc inhibits breast cancer cell plasticity and adaptation to stress
    Authors: O Balcioglu, RE Heinz, DW Freeman, BL Gates, BM Hagos, E Booker, E Mirzaei Me, HT Diesen, K Bhakta, S Ranganatha, M Kachi, M Leblanc, PC Gray, BT Spike
    Breast Cancer Res, 2020-11-13;22(1):125.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  2. Ascorbic acid promotes cardiomyogenesis through SMAD1 signaling in differentiating mouse embryonic stem cells
    Authors: MG Perino, S Yamanaka, DR Riordon, Y Tarasova, KR Boheler
    PLoS ONE, 2017-12-12;12(12):e0188569.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. CRIPTO/GRP78 signaling maintains fetal and adult mammary stem cells ex vivo.
    Authors: Spike B, Kelber J, Booker E, Kalathur M, Rodewald R, Lipianskaya J, La J, He M, Wright T, Klemke R, Wahl G, Gray P
    Stem Cell Reports, 2014-04-03;2(4):427-39.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  4. Role of Cripto-1 during epithelial-to-mesenchymal transition in development and cancer.
    Authors: Rangel MC, Karasawa H, Castro NP, Nagaoka T, Salomon DS, Bianco C
    Am. J. Pathol., 2012-04-26;180(6):2188-200.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Netrin-1 can affect morphogenesis and differentiation of the mouse mammary gland.
    Authors: Strizzi L, Mancino M, Bianco C, Raafat A, Gonzales M, Booth BW, Watanabe K, Nagaoka T, Mack DL, Howard B, Callahan R, Smith GH, Salomon DS
    J. Cell. Physiol., 2008-09-01;216(3):824-34.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Bioassay, In Vivo
  6. Canonical Wnt signaling is required for development of embryonic stem cell-derived mesoderm.
    Authors: Lindsley RC, Gill JG, Kyba M, Murphy TL, Murphy KM
    Development, 2006-08-30;133(19):3787-96.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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