Recombinant Mouse CKAP4/p63 Protein, CF

Catalog #: 9734-CK Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9734-CK-050

Recombinant Mouse CKAP4/p63 Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse CKAP4/p63 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse Dkk‑1 (Catalog # 5897-DK) is immobilized at 1 μg/mL (100 μL/well), the concentration of Recombinant Mouse CKAP4/p63 that produces 50% of the optimal binding response is 35-280 ng/mL.

Source

Mouse myeloma cell line, NS0-derived mouse CKAP4/p63 protein
Val109-Ile575, with an N-terminal 6-His tag

Accession #

AAH25522

N-terminal Sequence
Analysis

His

Predicted Molecular Mass

53 kDa

SDS-PAGE

45-59 kDa, reducing conditions

Product Datasheets

9734-CK

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9734-CK

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Mouse Dkk-1 (Catalog # 5897-DK) is immobilized at 1 µg/mL (100 µL/well), Recombinant Mouse CKAP4/p63 binds with an ED₅₀ of 35-280 ng/mL.

Reconstitution Calculator

Background: CKAP4/p63

CKAP4 (Cytoskeleton-associated protein 4; also CLIMP63 and p63) is a 63-64 kDa molecule that belongs to no known protein family. It is found intracellularly, and on the plasma membrane of select cells such as vascular smooth muscle and Type II Greater alveolar lung cells (1). CKAP4 has a bimodal distribution. First, it is embedded in the membrane of a compartment that links the ER with the Golgi apparatus (2). This localization is dependent upon its ability to form homooligomers, and its presence serves to anchor microtubules and direct the formation of tubular ER. Second, it is embedded in the plasma membrane and serves as a receptor for SP‑A/surfactant protein-A (in lung) (2) and tPA (in vessels) (3). Mouse CKAP4 is a 575 amino acid (aa) type II transmembrane nonglycosylated protein. It contains an 85 aa N-terminal cytoplasmic region plus a 467 aa C-terminal luminal domain (aa 109-575) (4). The luminal domain contains three coiled-coil regions (aa 125-193; 236‑438; 507-575) plus three utilized phosphorylation sites (5). CKAP4 undergoes reversible palmitoylation (6). Over aa 109-575, mouse CKAP4 shares 82% and 94% aa sequence identity with human and rat CKAP4, respectively. CKAP4 has been shown to be required for APF-mediated signaling (6-9), for gentamycin-induced cytotoxicity (8), for regulation of tPA function (3), and for SP-A-induced surfactant uptake by pneumocytes (10). CKAP4 interacts with Dickkopf-1 (Dkk-1) to promote activation of AKT-dependent, Wnt-independent signaling(11). Both CKAP4 and Dkk-1 are frequently up-regulated in pancreatic and lung cancers (11). Dkk-1 binds to CKAP4 with high affinity that is comparable to but independent of its interaction with LRP5/6 (11).

References

Bates, S. R. et al. (2008) Am J Physiol Lung Cell Mol Physiol, 295:L658.
Gupta, N. et al. (2006). Am J Physiol Lung Cell Mol Physiol 291:L436.
Razzaq, T. M. et al. (2003). J. Biol. Chem. 278:42679.
Schweizer, A. et al. (1994). J. Cell Biol.126:25.
Huttlin, E. L. et al. (2010) Cell 143:1174.
Planey, S. L. et al. (2009). Mol Biol Cell. 20:1454.
Conrads, T.P. (2006) J Bio Chem, 281:49.
Matika, C.A. (2012) Mol Bio Cell, 23:10.
Shahjee, H.M. (2010) J Exp and Clin Cancer Res, 29:1.
Kazi, A.S. (2010) Am J Physiol Lung Cell Mol Physiol 299:6.
Kimura, H. et al. (2016) J Clin Invest, 126:2689.

Long Name

Cytoskeleton-associated Protein-4/Climp-63

Entrez Gene IDs

10970 (Human); 216197 (Mouse); 362859 (Rat)

Alternate Names

63 kDa membrane protein; CKAP4; CLIMP-63; cytoskeleton-associated protein 4; ERGIC-63; MGC99554; p63; transmembrane protein (63kD), endoplasmic reticulum/Golgi intermediatecompartment; type-II transmembrane protein p63