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Recombinant Mouse CDNF Protein, CF

Catalog #: 5187-CD
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
5187-CD-050
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Citations (2)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse CDNF Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Able to significantly enhance neurite outgrowth when immobilized at 6-25 μg/mL on a nitrocellulose-coated microplate.
Source
Mouse myeloma cell line, NS0-derived mouse CDNF protein
Leu27-Leu187, with a C-terminal 10-His tag
Accession #
Q8CC36
N-terminal Sequence
Analysis
Leu27
Predicted Molecular Mass
19.8 kDa
SDS-PAGE
20 kDa, reducing conditions

Product Datasheets

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5187-CD

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5187-CD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CDNF

CDNF (conserved dopamine neurotrophic factor), also called Armetl1 (arginine-rich, mutated in early stage tumors-like 1), is a 17 - 19 kDa secreted protein that shares 52% amino acid (aa) identity with mouse MANF (mesencephalic-astrocyte-derived neurotrophic factor), also called Armet (1). The Armet designation is not preferred, because the proteins as translated are not actually arginine-rich (1). However, both CDNF and MANF have a high proportion of charged residues, a pattern of eight cysteines shown to form intramoleculular disulfide bonds, and a C-terminal endoplasmic reticulum retention signal (shown to function in MANF) (1 - 3). The mouse CDNF cDNA encodes a 187 aa protein with a 24 aa signal sequence and a 163 mature sequence (1). Mature mouse CDNF shares 80%, 87%, 83% and 82% aa identity with human, rat, equine and bovine CDNF, respectively. Although CDNF mRNA and protein are expressed in pre and postnatal mouse brain, they are mostly abundant in adult heart, skeletal muscle and testis. Transcripts within the postnatal mouse brain are concentrated in the hippocampus, thalamus, corpus callosum and optic nerve (1). Like MANF and GDNF, CDNF promotes survival of dopaminergic neurons in vitro (1, 4). In a rat Parkinson's disease model, CDNF also promotes rescue and restoration of dopaminergic neurons in vivo (1).

 

References
  1. Lindholm, P. et al. (2007) Nature 448:73.
  2. Mizobuchi, N. et al. (2007) Cell Struct. Funct. 32:41.
  3. Raykhel, I. et al. (2007) J. Cell Biol. 179:1193.
  4. Petrova, P. et al. (2003) J. Mol. Neurosci. 20:173.
Long Name
Conserved Dopamine Neurotrophic Factor
Entrez Gene IDs
441549 (Human); 227526 (Mouse); 361276 (Rat)
Alternate Names
arginine-rich, mutated in early stage tumors-like 1; ARMETL1; ARMET-like protein 1; CDNF; cerebral dopamine neurotrophic factor; Conserved dopamine neurotrophic factor

Citations for Recombinant Mouse CDNF Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. CDNF Interacts with ER Chaperones and Requires UPR Sensors to Promote Neuronal Survival
    Authors: A Eesmaa, LY Yu, H Göös, T Danilova, K Nõges, E Pakarinen, M Varjosalo, M Lindahl, P Lindholm, M Saarma
    International Journal of Molecular Sciences, 2022-08-22;23(16):.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA Standard
  2. Characterization of CDNF-Secreting ARPE-19 Cell Clones for Encapsulated Cell Therapy
    Authors: E Galli, P Lindholm, LS Kontturi, M Saarma, A Urtti, M Yliperttul
    Cell Transplant, 2019-03-06;28(4):413-424.
    Species: Human
    Sample Types: Protein
    Applications: ELISA Control

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