Recombinant Mouse CD43 Fc Chimera Protein, CF

Catalog #: 9959-CD Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9959-CD-050

Recombinant Mouse CD43 Fc Chimera Protein Binding Activity

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Product Details

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse CD43 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD43 Fc Chimera is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Mouse Siglec‑1/CD169 Fc Chimera (Catalog # 5610-SL) that produces 50% optimal binding response is 0.1-0.8 μg/mL.

Source

Mouse myeloma cell line, NS0-derived mouse CD43 protein

Mouse CD43 (Asp20-Gly248) Accession # P15702	IEGRMDP	Mouse IgG_2a (Glu98-Lys330)
N-terminus		C-terminus

Accession #

P15702

N-terminal Sequence
Analysis

Asp20

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

50 kDa

SDS-PAGE

99-118 kDa, reducing conditions

Product Datasheets

9959-CD

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9959-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	12 months from date of receipt, ≤ -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Mouse CD43 Fc Chimera is immobilized at 1 µg/mL, 100 µL/well, Recombinant Mouse Siglec-1/CD169 Fc Chimera (Catalog # 5610-SL) binds with an ED₅₀ of 0.1-0.8 µg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Mouse CD43 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 100-120 kDa and 200-220 kDa, respectively.

Reconstitution Calculator

Background: CD43

CD43, also known as Leukosialin, Sialophorin, B-cell differentiation antigen LP-3 and Ly-48, is a type I transmembrane sialylated mucin that is expressed on most leukocytes and some tumor cells (1). Notably, the membrane expression of CD43 seems to be a characteristic of leukocytes, while cytoplasmic expression without membrane insertion occurs in endothelium and select epithelia. While CD43 restricts leukocyte adhesion and modulates T cell activation, these activities are context specific (2). CD43 can both induce and protect against apoptosis, and can either promote or block cell adhesion (3). In mouse, CD43 is synthesized as a 395 amino acid (aa) precursor that contains a 19 aa signal sequence, a 229 aa extracellular region, a 23 aa TM domain, and a 124 aa cytoplasmic tail. The mouse extracellular region (aa 20-248) has only one N-linked glycosylation site while the human extracellular region (aa 20-253) has 25 O-linked glycosylation sites and one N-linked glycosylation site. Mouse CD43 extracellular region shares 38% and 61% aa sequence identity with the extracellular regions in human and rat CD43, respectively.

References

Santamaría, M. et al. (1996). Cancer Res. 56:3526.
Manjunath, N. et al. (1995) Nature 377:535.
Brown, T. J. et al. (1996) J. Biol. Chem. 271:27686.

Long Name

Cluster of Differentiation 43

Entrez Gene IDs

6693 (Human); 20737 (Mouse); 24796 (Rat); 102147271 (Cynomolgus Monkey)

Alternate Names

CD43 antigen; CD43; CD43); Galactoglycoprotein; GALGP; GPL115; Leukocyte sialoglycoprotein; Leukosialin; LSN; Ly-48; sialophorin (gpL115, leukosialin, CD43); Sialophorin; SPN