Recombinant Mouse CD19 Fc Chimera Protein, CF

Name: Recombinant Mouse CD19 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 9730-CD
Rating: 5 (2 reviews)

Catalog #: 9730-CD
1 Citation 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9730-CD-050

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Citations (1)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse CD19 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD19 Fc Chimera is immobilized at 2 µg/mL,100 µL/well, recombinant biotinylated human CD81 Fc Chimera binds with an ED₅₀ of 7-35 μg/mL

Source

Chinese Hamster Ovary cell line, CHO-derived mouse CD19 protein

Mouse CD19 (Arg19-Gly287) Accession # P25918	IEGRMDP	Mouse IgG_2a (Glu98-Lys330)
N-terminus		C-terminus

Accession #

P25918

N-terminal Sequence
Analysis

Arg19 and Ser68

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

57 kDa

SDS-PAGE

78-95 kDa, reducing conditions

Product Datasheets

9730-CD

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9730-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: CD19

CD19 is a 95 kDa transmembrane glycoprotein that plays a central role in B cell activation and humoral immune responses (1, 2). CD19 is expressed throughout B cell development from pre-B cells through mature B cells, and it is commonly used as a B cell lineage marker (1). It is required for the responsiveness of mature B cell to antigen stimulation, germinal center development, and antibody affinity maturation (4, 5). CD19 associates with the B cell antigen receptor (BCR), CD81, CD38, CD21, CD22, and IFITM1/CD225/Leu13, (6-9). These associations enable CD19 to amplify B cell signaling (7, 9-12) and reduce the threshold for antigen stimulation through the BCR (13). CD19 polymorphisms and up-regulation can lead to the development of autoimmunity by promoting autoantibody production (2). CD19 has emerged as promising therapeutic target for hematologic cancers and solid tumors. Immunotherapy using a chimeric antigen receptor (CAR) targeting CD19 has proven effective in many different cancers. The first CD19 CAR T cell therapies have been granted FDA approval for the treatment of B cell malignancies with several more in clinical trials. Mature human CD19 consists of a 272 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 22 aa transmembrane segment, and a 243 aa cytoplasmic domain (3). Within the ECD, human CD19 shares 57% amino acid sequence identity with mouse and rat CD19.

References

Wang, K. et al. (2012) Exp. Hematol. Oncol. 1:36.
Tedder, T.F. et al. (1997) Immunity 6:107.
Tedder, T.F. and C.M. Isaacs (1989) J. Immunol. 143:712.
Rickert, R.C. et al. (1995) Nature 376:352.
Engel, P. et al. (1995) Immunity 3:39.
Vences-Catalan, F. et al. (2012) J. Immunol. 137:48.
Deaglio, S. et al. (2007) Blood 109:5390.
Bradbury, L. et al. (1992) J. Immunol. 149:2841.
Krop, I. et al. (1996) J. Immunol. 157:48.
O'Rourke, L.M. et al. (1998) Immunity 8:635.
Buhl, A.M. et al. (1997) J. Exp. Med. 186:1897.
Sato, S. et al. (1995) Proc. Natl. Acad. Sci. USA 92:11558.
Carter, R.H. and D.T. Fearon (1992) Science 256:105.

Entrez Gene IDs

930 (Human); 12478 (Mouse); 365367 (Rat); 397669 (Porcine); 607898 (Canine); 102145514 (Cynomolgus Monkey); 100147468 (Equine)

Alternate Names

B4; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; CD19 antigen; CD19 molecule; CD19; CVID3; Differentiation antigen CD19; Leu-12; MGC12802; T-cell surface antigen Leu-12

Related Research Areas

Citation for Recombinant Mouse CD19 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Noncanonical binding of Lck to CD3&epsilon promotes TCR signaling and CAR function
Authors: FA Hartl, E Beck-Garcì, NM Woessner, LJ Flachsmann, RMV Cárdenas, SM Brandl, S Taromi, GJ Fiala, A Morath, P Mishra, OS Yousefi, J Zimmermann, N Hoefflin, M Köhn, BM Wöhrl, R Zeiser, K Schweimer, S Günther, WW Schamel, S Minguet
Nat. Immunol., 2020-07-20;21(8):902-913.
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture