Recombinant Mouse Cadherin-9 His-tag Protein, CF

Catalog #: 1456-CA Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
1456-CA-050

Recombinant Mouse Cadherin-9 His-tag Protein Binding Activity

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Product Details

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Cadherin-9 His-tag Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse Cadherin-9 is immobilized at 1 μg/mL, 100 μL/well, Recombinant Human Cadherin-6/KCAD Fc Chimera Recombinant Human Cadherin‑6/KCAD Fc Chimera (Catalog # 2715-CA) binds with an ED₅₀ of 0.25-1.5 μg/mL.

Source

Human embryonic kidney cell, HEK293-derived mouse Cadherin-9 protein

Mouse Cadherin-9 (Gly53-Ala614) Accession # P70407	HPGGGSGGGSGGGS	HHHHHH
N-terminus		C-terminus

Accession #

P70407

N-terminal Sequence
Analysis

Gly53

Predicted Molecular Mass

64.4 kDa

SDS-PAGE

75-85 kDa, under reducing conditions

Product Datasheets

1456-CA

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1456-CA

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 400 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	12 months from date of receipt, ≤ -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Mouse Cadherin 9 is immobilized at 1 µg/mL, 100 µL/well, Recombinant Human Cadherin-6/KCAD Fc Chimera Recombinant Human Cadherin-6/KCAD Fc Chimera (Catalog # 2715-CA) binds with an ED₅₀ of 0.25-1.5 µg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Mouse Cadherin-9 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 75-85 kDa.

Reconstitution Calculator

Background: Cadherin-9

Cadherin-9 (CDH9) is a member of the larger Cadherin superfamily of cell surface glycoproteins originally identified as proteins mediating cell-cell adhesion (1). In humans, there are more than 100 cadherin members divided into distinct families and numerous sub-families (1-3). Cadherins share a general structural architecture with an extracellular domain (ECD) containing 2 or more extracellular Ca2⁺ binding cadherin repeat (EC) domains, a single-pass transmembrane section, and a short cytoplasmic tail (1-3). Cadherins function by forming homophilic binding interactions through these EC domains to generate both trans and cis dimers (1-3). Mouse CDH9 is categorized as a classical cadherin, containing 5 EC domains, and the ECD shares 94% and 98% amino acid sequence identity with the ECD of human and rat CDH9, respectively. Cadherin-9 is expressed in human brain and kidney, in the limbic system of rats, and in testes and CD4/CD8-double-positive thymocytes of mice (4, 5). Cadherin-9 interacts with alpha and beta catenins, and also interacts with cadherin-6, 9, and 10 in cell aggregation assay (5). High levels of cadherin-9 is observed during renal fibrosis making cadherin-9 a key marker for fibroblasts in healthy and diseased kidney (4). Cadherin-9 plays a critical role in regulating the formation and differentiation of the hippocampal dentate gyrus (DG)-CA3 mossy fiber synapse (3). Loss of CDH9 expression leads to defects in synapse formation and differentiation of specific neural circuits (5). Additionally, disruption to CDH9 and CDH10 genes has been linked autism spectrum disorders (7, 8). Recently, CDH9 has been reported as a potential suppressor of cancer metastasis (9).

References