Recombinant Mouse C1qR1/CD93 Fc Chimera Protein, CF

Catalog #: 1696-CD Datasheet / COA / SDS

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1696-CD-050

R&D Systems Recombinant Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse C1qR1/CD93 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE with silver staining

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to induce TNF-alpha secretion by THP‑1 human acute monocytic leukemia cells. The ED₅₀ for this effect is 6-30 μg/mL.

Source

Mouse myeloma cell line, NS0-derived mouse C1q R1/CD93 protein

Mouse C1q R1 (Ala23-Asn572) Accession # O89103	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

O89103

N-terminal Sequence
Analysis

Ala23

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

85.7 kDa (monomer)

SDS-PAGE

120-130 kDa, reducing conditions

Product Datasheets

1696-CD

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1696-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 400 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: C1qR1/CD93

C1q R1, also known as CD93, C1qRp, and AA4, is an approximately 125 kDa transmembrane glycoprotein that is involved in various aspects of inflammatory reactions (1). Mature mouse CD93 consists of a 550 amino acid (aa) extracellular domain (ECD) that contains one C‑type lectin domain, five tandem EGF‑like repeats, and a mucin‑like region. This is followed by a 21 aa transmembrane segment and a 51 aa cytoplasmic domain (2, 3). Within the ECD, mouse CD93 shares 66% and 87% aa sequence identity with human and rat CD93, respectively. C1qR1/CD93 should not be confused with 60 kDa Calreticulin which is also known as C1q R1 (5, 6). Unlike Calreticulin, and despite its name, C1q R1/CD93 does not appear to bind the complement protein C1q (4, 6). CD93 is expressed by vascular endothelial cells (6) and by a variety of hematopoietic cells (4‑10). Various sized fragments of soluble CD93 (50‑75 kDa) can be shed from monocytes, neutrophils, and vascular endothelial cells following inflammatory stimulation, leaving a truncated form of the molecule embedded in the cell membrane (12‑14). Cross‑linking of cell surface CD93 enhances phagocytosis by monocytes, and enhances the uptake of apoptotic cells in vivo (11, 16). Soluble CD93 promotes the differentiation of monocytes to macrophages, the phagocytosis of apoptotic cells, and inflammatory responsiveness to multiple TLR ligands (13, 15). It is required for plasma cell longevity in the bone marrow, resulting in an extended humoral response (10).

References

Greenlee-Wacker, M.C. et al. (2012) Curr. Drug Targets 13:411.
Petrenko, O. et al. (1999) Immunity 10:691.
Norsworthy, P.J. et al. (1999) Mamm. Genome 10:789.
Steinberger, P. et al. (2002) J. Leukoc. Biol. 71:133.
Nepomuceno, R.R. and A.J. Tenner (1998) J. Immunol. 160:1929.
McGreal, E.P. et al. (2002) J. Immunol. 168:5222.
Lovik, G. et al. (2001) Scand. J. Immunol. 53:410.
Danet, G.H. et al. (2002) Proc. Natl. Acad. Sci. USA 99:10441.
Ikewaki, N. et al. (2010) J. Clin. Immunol. 30:723.
Chevrier, S. et al. (2009) Proc. Natl. Acad. Sci. USA 106:3895.
Norsworthy, P.J. et al. (2004) J. Immunol. 172:3406.
Bohlson, S.S. et al. (2005) J. Immunol. 175:1239.
Greenlee, M.C. et al. (2009) Inflamm. Res. 58:909.
Greenlee-Wacker, M.C. et al. (2011) J. Immunol. 187:3353.
Jeon, J.W. et al. (2010) J. Immunol. 185:4921.
Nepomuceno, R.R. et al. (1999) J. Immunol. 162:3583.

Long Name

Complement Component C1q Receptor

Entrez Gene IDs

22918 (Human); 17064 (Mouse); 84398 (Rat)

Alternate Names

AA4 Antigen; C1q R1; C1q receptor 1; C1q Rp; C1q/MBL/SPA receptor; C1qR(P); C1qR1; C1qRp; CD93 antigenC1qR; CD93 molecule; CD93; CDw93C1QR1; Collectin Receptor; Complement component 1 q subcomponent receptor 1; complement component 1, q subcomponent, receptor 1; complement component C1q receptor; dJ737E23.1; ECSM3; Ly68; Matrix-remodeling-associated protein 4; matrix-remodelling associated 4; MXRA4