Recombinant Mouse BTNL9 Fc Chimera Protein, CF
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Recombinant Mouse BTNL9 Fc Chimera Protein, CF Summary
Product Specifications
Mouse BTNL9 (Asp36-Lys257) Accession # Q8BJE2-1 | IEGRMDP | Mouse IgG2a (Glu98-Lys330) |
N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9555-BT
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution | Reconstitute at 200 μg/mL in PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
![Recombinant Mouse BTNL9 Fc Chimera Protein Bioactivity Recombinant Mouse BTNL9 Fc Chimera Protein Bioactivity](https://aeroglobalimagesuat.blob.core.windows.net/images/datasheets/protein/BTNL9_9555BT_1646.jpg)
Recombinant Mouse BTNL9 Fc Chimera (Catalog # 9555-BT) inhibits IL-2 secretion by mouse T cells in the presence of anti-CD antibody. The ED50 for this effect is 0.75-4.5 μg/mL.
Reconstitution Calculator
Background: BTNL9
Butyrophilin-like 9 (BTNL9) is a member of the BTN/MOG Ig-superfamily and functions as a negative regulator of immune cell activation (1). Mouse BTNL9 is a 536 amino acid (aa) type I transmembrane glycoprotein that contains a 35 aa signal sequence, a 222 aa extracellular domain (ECD), a 21 aa transmembrane region and a 258 aa cytoplasmic segment. The ECD features two V-type Ig-like domains and three glycosylation sites (2-4). The ECD of mouse BTNL9 shares ~67% and 91% aa sequence identity with the ECD of human and rat BTNL9, respectively. BTNL9 is expressed in a variety of tissues in humans and mice. Among isolated immune cell types, B cells show the strongest expression (2, 5). Recombinant BTNL9–Fc has been shown to bind to many immune cells including T cells, B cells, macrophages and dendritic cells (6). With this profile of binding, it will not be surprising if BTNL9 joined the panel of butyrophilins with negative regulatory effects on immune cells (5). BTNL9 has been reported to inhibit CD8+ T Cell proliferation (5).
- Arnett, H.A. et al. (2007) J. Immunol. 178:1523
- Arnett, H.A. et al. (2009) Cytokine 46:370.
- Chapoval, A.I. et al. (2013) Mol. Immunol. 56:819.
- Abeler-Dorner, L. et al. (2012) Trends Immunol. 33:34.
- Arnett, H.A. et al. (2014) Nat Rev Immunol. 14:5596.
- Yamazaki, T. et al. (2010) J. Immunol. 185:5907.
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