Recombinant Mouse Angiopoietin-like 3 (HA-Tag) Protein, CF

Catalog # Availability Size / Price Qty
9899-AN-050
Recombinant Mouse Angiopoietin-like 3 (HA-Tag) Protein Bioactivity
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Recombinant Mouse Angiopoietin-like 3 (HA-Tag) Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit lipoprotein lipase activity. Yoshida, K. et al. (2002) J. Lipid Res. 43:1770. The IC50 value under conditions in which Recombinant Human Lipoprotein Lipase  (Catalog # 9888-LL) is present at 0.5 μg/mL, and p‑nitrophenyl butyrate concentration is 1 mM in 0.1 M sodium phosphate, 0.15 M NaCl, 0.5% Triton® X-100, pH 7.2, is 2-10 µg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Angiopoietin-like Protein 3/ANGPTL3 protein
Hemagglutinin Tag
(YPYDVPDYA)
Mouse Angiopoietin-like Protein 3
(Ser17-Thr455)
Accession # Q9R182
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Tyr
Predicted Molecular Mass
52 kDa
SDS-PAGE
59-71 kDa and 33-38 kDa, reducing conditions

Product Datasheets

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9899-AN

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9899-AN

Formulation Lyophilized from a 0.2 μm filtered solution in PBS, NaCl and CHAPS with Trehalose.
Reconstitution Reconstitute at 400 μg/mL in water.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Mouse Angiopoietin-like 3 (HA-Tag) Protein Bioactivity View Larger

Recombinant Mouse ANGPTL3 (Catalog # 9899-AN) dose dependently inhibits Recombinant Human LPL (Catalog # 9888-LL) activity with an IC50of 2-10 µg/mL.

SDS-PAGE Recombinant Mouse Angiopoietin-like 3 (HA-Tag) Protein SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse Angiopoietin‑like Protein 3 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 59 - 71 kDa and 33 - 38 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Angiopoietin-like Protein 3/ANGPTL3

Angiopoietin-like 3 (ANGPTL3) is a secreted glycoprotein that is structurally related to the angiopoietins (1-3). Mature mouse ANGPTL3 contains an N-terminal coiled‑coil domain and a C‑terminal fibrinogen-like domain (4). ANGPTL3 is expressed in the liver from early in development through adulthood (4, 5). Full length ANGPTL3 circulates in the plasma as do the proteolytically separated N- and C‑terminal fragments containing the coiled‑coil domain and fibrinogen-like domains, respectively (6, 7). ANGPTL3 is found as 70 kDa, 50 kDa, and 32 kDa species and can form weakly associated noncovalent multimers in vitro (5, 6). ANGPTL3 directly inhibits lipoprotein lipase (LPL), an enzyme responsible for hydrolyzing circulating triglycerides (8). This activity requires a putative heparin-binding motif that is N-terminal to the coiled‑coil domain (6). Proteolytic removal of the fibrinogen-like domain from the N-terminal fragment serves to activate ANGPTL3 and increase its ability to inhibit LPL in vitro and function in vivo (6). ANGPTL3 promotes an increase in circulating triglyceride levels without altering VLDL or HDL secretion or uptake (6-8). ANGPTL3 knockout mice are hypolipidemic and have elevated LPL activity (9). ANGPTL3 expression in vivo is up‑regulated by LXR agonists and down-regulated by insulin, leptin, and TR beta agonists (10-12). Dysregulated ANGPTL3 expression and elevated plasma triglyceride levels are characteristic of some strains of obese and diabetic mice, (7, 8, 11). ANGPTL3 does not bind Tie-1 or Tie-2 but its fibrinogen-like domain interacts with integrin alpha V beta 3 to induce endothelial cell adhesion, migration, and neovascularization (13). ANGPTL3, secreted by fetal liver cells, also promotes the expansion of hematopoietic stem cells (14). Mature mouse ANGPTL3 shares 22% - 30% amino acid (aa) sequence identity with ANGPTL1, 2, 4, 6, and 7. It shares 77% aa sequence identity with human ANGPTL3.

References
  1. Li, C. (2006) Curr. Opin. Lipidol. 17:152.
  2. Oike, Y. et al. (2004) Int. J. Hematol. 80:21.
  3. Kersten, S. (2005) Biochem. Soc. Transact. 33:1059.
  4. Conklin, D. et al. (1999) Genomics 62:477.
  5. Ge, H. et al. (2005) J. Lipid Res. 46:1484.
  6. Ono, M. et al. (2003) J. Biol. Chem. 278:41804.
  7. Koishi, R. et al. (2002) Nat. Genet. 30:151.
  8. Shimizugawa, T. et al. (2002) J. Biol. Chem. 277:33742.
  9. Koster, A. et al. (2005) Endocrinology 146:4943.
  10. Inaba, T. et al. (2003) J. Biol. Chem. 278:21344.
  11. Shimamura, M. et al. (2004) Biochem. Biophys. Res. Commun. 322:1080.
  12. Fugier, C. et al. (2006) J. Biol. Chem. 281:11553.
  13. Camenisch, G. et al. (2002) J. Biol. Chem. 277:17281.
  14. Zhang, C.C. et al. (2006) Nat. Med. 12:240.
Entrez Gene IDs
27329 (Human); 30924 (Mouse); 102136264 (Cynomolgus Monkey)
Alternate Names
AGNPT5; Ang-5; angiopoietin 5; Angiopoietin-5; angiopoietin-like 3; Angiopoietin-like Protein 3; angiopoietin-related protein 3; ANGPT5angiopoietin-5; ANGPTL3; FHBL2

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