Recombinant Mouse Angiopoietin-1 Protein Summary
Product Specifications
| Mouse Angiopoietin-1 (Ser20-Leu261) Accession # O08538 | GGGSGGGSGGGS | Mouse Angiopoietin-1 (Arg277-Phe498) Accession # O08538 | HHHHHH |
| N-terminus | C-terminus | ||
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9936-AN
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl with Trehalose and with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 250 μg/mL in water. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: |
|
9936-AN/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl with Trehalose. |
| Reconstitution | Reconstitute at 250 μg/mL in water. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: |
|
Scientific Data
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Recombinant Mouse Angiopoietin-1 inhibits serum deprivation induced apoptosis in HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 10-50 ng/mL in the presence of 5 µg/mL of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050).
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2 μg/lane of Recombinant Mouse Angiopoietin‑1 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 59-75 kDa and oligomer, respectively.
Reconstitution Calculator
Background: Angiopoietin-1
Angiopoietin-1 (Ang-1) is a secreted glycoprotein that plays a critical role in the development and maintenance of the vascular system (1, 2). It contains a N-terminal coiled-coil region and a C-terminal fibrinogen-like domain separated by a short flexible region (3, 4). Mature Mouse Angiopoietin-1 shares 97% and 98% amino acid sequence identity with Human and rat Angiopoietin-1, respectively. It is expressed by vascular smooth muscle cells and pericytes as an approximately 70 kDa molecule that associates into disulfide-linked homotrimers, tetramers, and pentamers (3, 5). Angiopoietin-1 binds and activates the receptor tyrosine kinase Tie-2, and its association into tetramers is important for full Tie-2 activation (3, 4). Angiopoietin-1 ligation of Tie-2 on vascular endothelial cells (EC) induces the development and branching of blood vessels (6, 7). In sub-confluent EC (i.e. during angiogenesis), Angiopoietin-1 promotes EC motility and Tie-2 localization at the trailing edge of the cell (8). In confluent EC (i.e. in homeostasis), multimeric Angiopoietin-1 enhances vascular integrity by promoting the in trans homotypic association of Tie-2 between EC or with the substratum (8, 9). In addition, Angiopoietin-1 suppresses several VEGF-induced effects on the vasculature including endothelial permeability, stretch-induced release of Angiopoietin-2, and up-regulation of the leukocyte adhesion molecules VCAM-1, ICAM-1, and E-Selectin (10-12). Angiopoietin-1 also interacts with a variety of integrins and the extracellular matrix independently of Tie-2 (13, 14). These interactions support the adhesion, migration and stress resistance of EC, fibroblasts, and myocytes (13, 14). Angiopoietin-1 can protect against pulmonary arterial hypertension (5), reduce the extent of fibrosis and remodeling in infarcted diabetic myocardium (15), and enhance tumor progression and metastasis (16).
- Koh, G.Y. (2012) Trends Mol. Med. 19:31.
- Suri, C. et al. (1996) Cell 87:1171.
- Davis, S. et al. (1996) Cell 87:1161.
- Kim, K.-T. et al. (2005) J. Biol. Chem. 280:20126.
- Kugathasan, L. et al. (2009) J. Exp. Med. 206:2221.
- Suri, C. et al. (1998) Science 282:468.
- Jeansson, M. et al. (2011) J. Clin. Invest. 121:2278.
- Saharinen, P. et al. (2008) Nat. Cell. Biol. 10:527.
- Fukuhara, S. et al. (2008) Nat. Cell Biol. 10:513.
- Jho, D. et al. (2005) Circ. Res. 96:1282.
- Korff, T. et al. (2012) Cardiovasc. Res. 94:510.
- Kim, I. et al. (2001) Circ. Res. 89:477.
- Carlson, T.R. et al. (2001) J. Biol. Chem. 276:26516.
- Dallabrida, S.M. et al. (2005) Cardiovasc. Res. 96:e8.
- Samuel, S.M. et al. (2010) Diabetes 59:51.
- Holopainen, T. et al. (2009) Cancer Res. 69:4656.
Citations for Recombinant Mouse Angiopoietin-1 Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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The angiopoietin-Tie2 pathway regulates Purkinje cell dendritic morphogenesis in a cell-autonomous manner
Authors: R Luck, A Karakatsan, B Shah, G Schermann, H Adler, J Kupke, N Tisch, HW Jeong, MK Back, F Hetsch, A D'Errico, M De Palma, E Wiedtke, D Grimm, A Acker-Palm, J von Engelh, RH Adams, HG Augustin, C Ruiz de Al
Cell Reports, 2021-08-17;36(7):109522.
Species: Mouse
Sample Types: Organoid
Applications: Bioassay -
Neuromuscular Activity Induces Paracrine Signaling and Triggers Axonal Regrowth after Injury in Microfluidic Lab-On-Chip Devices
Authors: J Sala-Jarqu, F Mesquida-V, M Badiola-Ma, J Samitier, A Hervera, JA Del Río
Cells, 2020-01-27;9(2):.
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture
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