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Recombinant Mouse Amphiregulin Protein

Catalog #: 989-AR
26 Citations 2 Reviews Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
989-AR-100/CF

With Carrier

Catalog # Availability Size / Price Qty
989-AR-100
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (26)
FAQs
Reviews (2)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Amphiregulin Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Marquardt, H. et al. (1984) Science 223:1079. The ED50 for this effect is 5-20 ng/mL.
Source
E. coli-derived mouse Amphiregulin protein
Ser94-Lys191
Accession #
Q4FJT2
N-terminal Sequence
Analysis
Ser94
Predicted Molecular Mass
11.3 kDa

Product Datasheets

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989-AR (with carrier)

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COA
SDS

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989-AR/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

989-AR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

989-AR/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Amphiregulin

Amphiregulin (AR), also known as Schwannoma-derived growth factor (SDGF), is a member of the epidermal growth factor (EGF) family of growth factors which includes, AR, EGF, transforming growth factor-a (TGF-a), heparin binding EGF-like growth factor (HB-EGF), betacellulin (BTC), epiregulin, and the neuregulins-1 through -4. All EGF family members are synthesized as type I transmembrane precursors and contain one or several EGF domains in their extracellular region. The bioactive form of the proteins is released by proteolytic cleavage. The ErbB family of receptors that includes ErbB1 - B4, mediates the biological activities of the EGF family ligands. AR was originally isolated from the conditioned media of PMA-treated MCF-7 human breast carcinoma cell line. AR mRNA expression can be detected in numerous carcinoma cell lines and in the epithelial cells of various human tissues including colon, stomach, breast, ovary and kidney. AR stimulates the proliferation of keratinocytes, mammary epithelial cells, fibroblasts, astrocytes and glial cells. AR is also a growth inhibitor for certain tumor cells. The gene for AR has been mapped to human chromosome 4q13-q21 and mouse chromosome 5. Human and mouse AR cDNA encode 252 and 248 amino acid residue type I membrane proteins, respectively. The two proteins share approximately 69% sequence identity. Mouse AR also shares 81% amino acid sequence homology with rat AR. Several secreted isoforms of AR that vary in length and/or glycosylation level can be found in cell conditioned media. The 98 amino acid residue recombinant AR has better receptor binding and biological activity than the C-terminal truncated forms of the protein.

References
  1. Thompson, S.A. et al. (1996) J. Biol. Chem. 271:17927.
  2. Sonoda, H. et al. (1992) Biochem. Biophys. Res. Commun. 185:103.
  3. Normanno, N. et al. (2001) Frontiers in Bioscience 6:685.
Entrez Gene IDs
374 (Human); 11839 (Mouse)
Alternate Names
Amphiregulin; AR; AREG; AREGB; Colorectum cell-derived growth factor; CRDGF; MGC13647; schwannoma-derived growth factor; SDGF

Citations for Recombinant Mouse Amphiregulin Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

26 Citations: Showing 1 - 10
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  1. Heparan sulfate regulates amphiregulin signaling towards reparative lung mesenchymal cells during influenza A infection
    Authors: Loffredo, LF;Surpur, A;Ringham, OR;Li, F;de Los Santos-Alexis, K;Arpaia, N;
    bioRxiv : the preprint server for biology
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Investigating the impact of antibiotic-induced dysbiosis on protection from Clostridium difficile colitis by mouse colonic innate lymphoid cells
    Authors: Uddin, MJ;Thompson, B;Leslie, JL;Fishman, C;Sol-Church, K;Kumar, P;Petri, WA;
    mBio
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  3. Unique adipose tissue invariant natural killer T cell subpopulations control adipocyte turnover in mice
    Authors: Han, SM;Park, ES;Park, J;Nahmgoong, H;Choi, YH;Oh, J;Yim, KM;Lee, WT;Lee, YK;Jeon, YG;Shin, KC;Huh, JY;Choi, SH;Park, J;Kim, JK;Kim, JB;
    Nature communications
    Species: Mouse, Transgenic Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  4. CAPA-IVM improves the cytoplasmic quality of in vitro-matured oocytes from unstimulated mice
    Authors: Gong, X;Shen, L;Zhang, H;Ai, J;Gilchrist, RB;Zhao, Y;
    Theriogenology
    Species: Mouse
    Sample Types: Whole Cells
    Applications: In vitro
  5. An unanticipated discourse of HB-EGF with VANGL2 signaling during embryo implantation
    Authors: Kim, YS;Yuan, J;Dewar, A;Borg, JP;Threadgill, DW;Sun, X;Dey, SK;
    Proceedings of the National Academy of Sciences of the United States of America
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Bioassay
  6. Non-enzymatic role of SOD1 in intestinal stem cell growth
    Authors: YC Wang, XX Leng, CB Zhou, SY Lu, CK Tsang, J Xu, MM Zhang, HM Chen, JY Fang
    Cell Death & Disease, 2022-10-20;13(10):882.
    Species: Mouse
    Sample Types: Organoid
    Applications: Bioassay
  7. Amphiregulin mediates non-cell-autonomous effect of senescence on reprogramming
    Authors: M von Joest, C Chen, T Douché, J Chantrel, A Chiche, QG Gianetto, M Matondo, H Li
    Oncogene, 2022-07-12;40(2):111074.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  8. Cardiac macrophages prevent sudden death during heart stress
    Authors: J Sugita, K Fujiu, Y Nakayama, T Matsubara, J Matsuda, T Oshima, Y Liu, Y Maru, E Hasumi, T Kojima, H Seno, K Asano, A Ishijima, N Tomii, M Yamazaki, F Kudo, I Sakuma, R Nagai, I Manabe, I Komuro
    Nature Communications, 2021-03-26;12(1):1910.
    Species: Human, Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Bioassay
  9. Mesenchymal Stem and Stromal Cells Harness Macrophage-Derived Amphiregulin to Maintain Tissue Homeostasis
    Authors: JH Ko, HJ Kim, HJ Jeong, HJ Lee, JY Oh
    Cell Rep, 2020-03-17;30(11):3806-3820.e6.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  10. AhR controls redox homeostasis and shapes the tumor microenvironment in BRCA1-associated breast cancer
    Authors: SP Kubli, C Bassi, C Roux, A Wakeham, C Göbl, W Zhou, SM Jafari, B Snow, L Jones, L Palomero, KL Thu, L Cassetta, D Soong, T Berger, P Ramachandr, SP Baniasadi, G Duncan, M Lindzen, Y Yarden, C Herranz, C Lazaro, MF Chu, J Haight, P Tinto, J Silvester, DW Cescon, A Petit, S Pettersson, JW Pollard, TW Mak, MA Pujana, P Cappello, C Gorrini
    Proc. Natl. Acad. Sci. U.S.A., 2019-02-07;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Loss of amphiregulin reduces myoepithelial cell coverage of mammary ducts and alters breast tumor growth
    Authors: SPH Mao, M Park, RM Cabrera, JR Christin, GS Karagianni, MH Oktay, DMW Zaiss, SI Abrams, W Guo, JS Condeelis, PA Kenny, JE Segall
    Breast Cancer Res., 2018-10-26;20(1):131.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Hypoxia-inducible factor 2-alpha-dependent induction of amphiregulin dampens myocardial ischemia-reperfusion injury
    Authors: M Koeppen, JW Lee, SW Seo, KS Brodsky, S Kreth, IV Yang, PM Buttrick, T Eckle, HK Eltzschig
    Nat Commun, 2018-02-26;9(1):816.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  13. Inductions of granulosa cell luteinization and cumulus expansion are dependent on the fibronectin-integrin pathway during ovulation process in mice
    Authors: H Kitasaka, T Kawai, SAM Hoque, T Umehara, Y Fujita, M Shimada
    PLoS ONE, 2018-02-08;13(2):e0192458.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Epiregulin and EGFR interactions are involved in pain processing
    Authors: LJ Martin, SB Smith, A Khoutorsky, CA Magnussen, A Samoshkin, RE Sorge, C Cho, N Yosefpour, S Sivaselvac, S Tohyama, T Cole, TM Khuong, E Mir, DG Gibson, JS Wieskopf, SG Sotocinal, JS Austin, CB Meloto, JH Gitt, C Gkogkas, N Sonenberg, JD Greenspan, RB Fillingim, R Ohrbach, GD Slade, C Knott, R Dubner, AG Nackley, A Ribeiro-da, GG Neely, W Maixner, DV Zaykin, JS Mogil, L Diatchenko
    J. Clin. Invest., 2017-08-07;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  15. Amphiregulin contained in NSCLC-exosomes induces osteoclast differentiation through the activation of EGFR pathway
    Authors: S Taverna, M Pucci, M Giallombar, MA Di Bella, M Santarpia, P Reclusa, I Gil-Bazo, C Rolfo, R Alessandro
    Sci Rep, 2017-06-09;7(1):3170.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Overactive EGFR Signaling Leads to Increased Fibrosis After SARS-CoV Infection
    Authors: T Venkataram, C Coleman, M Frieman
    J. Virol., 2017-05-26;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  17. A heart-brain-kidney network controls adaptation to cardiac stress through tissue macrophage activation
    Authors: K Fujiu, M Shibata, Y Nakayama, F Ogata, S Matsumoto, K Noshita, S Iwami, S Nakae, I Komuro, R Nagai, I Manabe
    Nat. Med., 2017-04-10;23(5):611-622.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  18. Amphiregulin Antibody and Reduction of Axial Elongation in Experimental Myopia
    Authors: WJ Jiang, HX Song, SY Li, B Guo, JF Wu, GP Li, DD Guo, L Shi, HS Bi, JB Jonas
    EBioMedicine, 2017-02-23;0(0):.
    Species: Guinea Pig
    Sample Types: In Vivo
    Applications: Neutralization
  19. Progesterone-Based Therapy Protects Against Influenza by Promoting Lung Repair and Recovery in Females
    PLoS Pathog, 2016-09-15;12(9):e1005840.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  20. Bone Marrow CD11c+ Cell-Derived Amphiregulin Promotes Pulmonary Fibrosis
    J Immunol, 2016-05-20;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  21. RAF inhibitors that evade paradoxical MAPK pathway activation.
    Authors: Zhang C, Spevak W, Zhang Y, Burton E, Ma Y, Habets G, Zhang J, Lin J, Ewing T, Matusow B, Tsang G, Marimuthu A, Cho H, Wu G, Wang W, Fong D, Nguyen H, Shi S, Womack P, Nespi M, Shellooe R, Carias H, Powell B, Light E, Sanftner L, Walters J, Tsai J, West B, Visor G, Rezaei H, Lin P, Nolop K, Ibrahim P, Hirth P, Bollag G
    Nature, 2015-10-14;526(7574):583-6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Temporal expression of growth factors triggered by epiregulin regulates inflammation development.
    Authors: Harada M, Kamimura D, Arima Y, Kohsaka H, Nakatsuji Y, Nishida M, Atsumi T, Meng J, Bando H, Singh R, Sabharwal L, Jiang J, Kumai N, Miyasaka N, Sakoda S, Yamauchi-Takihara K, Ogura H, Hirano T, Murakami M
    J Immunol, 2015-01-02;194(3):1039-46.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  23. A special population of regulatory T cells potentiates muscle repair.
    Authors: Burzyn D, Kuswanto W, Kolodin D, Shadrach J, Cerletti M, Jang Y, Sefik E, Tan T, Wagers A, Benoist C, Mathis D
    Cell, 2013-12-05;155(6):1282-95.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  24. STAT3 regulates uterine epithelial remodeling and epithelial-stromal crosstalk during implantation.
    Authors: Pawar S, Starosvetsky E, Orvis G, Behringer R, Bagchi I, Bagchi M
    Mol Endocrinol, 2013-10-07;27(12):1996-2012.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Bioassay
  25. Amphiregulin-dependent mucous cell metaplasia in a model of nonallergic lung injury.
    Authors: Manzo, Nicholas, Foster, W Michae, Stripp, Barry R
    Am J Respir Cell Mol Biol, 2012-04-05;47(3):349-57.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  26. Innate lymphoid cells promote lung-tissue homeostasis after infection with influenza virus.
    Authors: Monticelli LA, Sonnenberg GF, Abt MC
    Nat. Immunol., 2011-11-01;12(11):1045-54.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Bioassay, In Vivo

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Recombinant Mouse Amphiregulin Protein
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Application: In vivo study
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