Recombinant Mouse Activin RIIB Fc Chimera Protein, CF
Recombinant Mouse Activin RIIB Fc Chimera Protein, CF Summary
Product Specifications
| Mouse Activin RIIB (Ser19-Thr134) Accession # P27040 |
IEGRMDP | Mouse IgG2A (Glu98-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
3725-RB
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Scientific Data
View Larger
Recombinant Mouse Activin RIIB Fc Chimera (Catalog # 3725-RB) inhibits Activin A-induced hemoglobin expression in the K562 human chronic myelogenous leukemia cell line. The ED50 for this effect is 5-30 ng/mL in the presence of 3 ng/mL of Recombinant Human/Mouse/Rat Activin A (Catalog # 338-AC).
View Larger
1 μg/lane of Recombinant Mouse Activin RIIB Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 63 kDa and 110 kDa, respectively.
Reconstitution Calculator
Background: Activin RIIB
Activin molecules and other members of the TGF-beta superfamily exert their biological effects through heteromeric complexes of type I and a type II serine/threonine kinase receptors (1, 2). Ligands bind to the type II receptor (such as ActRIIB) which then associates with a type I receptor to initiate signal transduction (3, 4). The mature mouse ActRIIB consists of a 119 amino acid (aa) extracellular domain, a 21 aa transmembrane segment, and a 378 aa cytoplasmic region that includes the kinase domain and a C-terminal PDZ-binding motif (5). ActRIIB exists in four alternately spliced forms that are distinguished by the deletion of juxtamembrane stretches in the extra and/or intracellular regions (5). Particular activin isoforms bind with different high affinities to the various type II receptor isoforms (5). Within the ECD, mouse ActRIIB shares 99% aa sequence identity with human and rat ActRIIB. It shares 53% aa sequence identity with ActRIIA. Besides the activin isoforms, ActR-II will also bind inhibin, BMP-2, and BMP-7 with lower affinities. ActRIIB mediated signaling can be modulated by several accessory proteins. Interactions with the GPI linked RGM-A/DRAGON lower the threshold for BMP-2 and BMP-4 induced signaling (6). ActRIIB forms a ternary complex with activin A and cripto which prevents association with the type I receptor ActRIB, thereby interfering with activin induced signaling (7). ActRIIB can also form a ternary complex with activin A and endoglin (8). The C-terminal tail of ActRIIB specifically binds the PDZ domain of the cytoplasmic protein ARIP2 which enhances receptor internalization and hinders activin induced responses (9). Although three variants of ARIP2 have been identified, only the full length isoform modulates ActRII internalization (10).
- Welt, C. et al. (2002) Exp. Biol. Med. 227:724.
- Chen, Y.-C., et al. (2006) Exp. Biol. Med. 231:534.
- Attisano, L. et al. (1996) Mol. Cell Biol. 16:1066.
- Allendorph, G.P. et al. (2006) Proc. Natl. Acad. Sci. 103:7643.
- Attisano, L. et al. (1992) Cell 68:97.
- Samad, T.A. et al. (2005) J. Biol. Chem. 280:14122.
- Gray, P.C. et al. (2003) Proc. Natl. Acad. Sci. 100:5193.
- Barbara, N.P. et al. (1999) J. Biol. Chem. 274:584.
- Matsuzaki, T. et al. (2002) J. Biol. Chem. 277:19008.
- Liu, Z.H. et al. (2006) J. Endocrinol. 189:409.
Citations for Recombinant Mouse Activin RIIB Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
2
Citations: Showing 1 - 2
Filter your results:
Filter by:
-
Muscular atrophy of caveolin-3-deficient mice is rescued by myostatin inhibition.
Authors: Ohsawa Y, Hagiwara H, Nakatani M, Yasue A, Moriyama K, Murakami T, Tsuchida K, Noji S, Sunada Y
J. Clin. Invest., 2006-10-12;116(11):2924-34.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Retinal neurons regulate proliferation of postnatal progenitors and Muller glia in the rat retina via TGF beta signaling.
Authors: Close JL, Gumuscu B, Reh TA
Development, 2005-07-01;132(13):3015-26.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for Recombinant Mouse Activin RIIB Fc Chimera Protein, CF
Average Rating: 5 (Based on 1 Review)
Have you used Recombinant Mouse Activin RIIB Fc Chimera Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
