Recombinant Human Xylulokinase/XYLB Protein, CF

Catalog #: 8267-XB Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
8267-XB-020

R&D Systems Recombinant Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Xylulokinase/XYLB Protein, CF Summary

Product Specifications

Endotoxin Level

<1.000 EU per 1 µg of the protein by the LAL method.

Activity

Measured by its ability to phosphorylate D-Xylulose.
The specific activity is >6,000 pmol/min/μg, as measured under the described conditions.

Accession #

O75191

N-terminal Sequence
Analysis

Met

Predicted Molecular Mass

59 kDa

SDS-PAGE

56 kDa, reducing conditions

Product Datasheets

8267-XB

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8267-XB

Formulation	Supplied as a 0.2 μm filtered solution in Tris, NaCl, DTT and Glycerol.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Reconstitution Calculator

Background: Xylulokinase/XYLB

D-Xylulokinase (XYLB) catalyzes the phosphorylation of D-xylulose (Xu) to produce xylulose-5-phosphate (Xu5P) mainly in liver and kidney and is inhibited by 5‑deoxy‑5‑fluoro‑D‑xylulose (1). XYLB is the last enzyme in the glucuronate-xylulose pathway, and its product Xu5P interfaces with the pentose phosphate pathway. XYLB belongs to a family of enzymes that also include fucokinase, gluconokinase and glycerokinase. Recently, the metabolic pathway has re-emerged as a focus for drug discovery in cancer research as rapidly growing malignant tumor cells typically have glycolytic rates up to 200 times higher than those of their normal tissues of origin, i.e. the Warburg effect (2, 3). Metabolic enzymes, such as PKM2 (4) and phosphofructokinase 1 (5), are being explored as drug targets. Given that Xu5P is a key regulator of glucose metabolism and lipogenesis (1), XYLB could also be an attractive drug target. The kinase activity was measured using a phosphatase-coupled method (6).

References

Bunker, R.D. et al. (2013) J. Biol. Chem. 288:1643.
Gatenby, R.A. and Gillies, R.J. (2004). Nat. Rev. Cancer 4:891.
Kim, J.W. and Dang, C.V. (2006). Cancer Res. 66:8927.
Israelsen, W.J. et al. (2013) Cell 155:397.
Yi, W. et al. (2012) Science 337:975.
Wu, Z. (2011) PLoS ONE 6:e23172.

Entrez Gene IDs

9942 (Human); 102448 (Mouse); 316067 (Rat)

Alternate Names

EC 2.7.1.17; FLJ10343; FLJ12539; FLJ22075; XYLB; xylulokinase (H. influenzae) homolog; xylulokinase homolog (H. influenzae); Xylulokinase; Xylulose Kinase