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Recombinant Human Wnt-11 Protein

Catalog #: 6179-WN
20 Citations 1 Review Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
6179-WN-010/CF

With Carrier

Catalog # Availability Size / Price Qty
6179-WN-010
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (20)
FAQs
Reviews (1)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Wnt-11 Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation/survival assay using IEC‑18 rat small intestinal epithelial cells. The ED50 for this effect is 0.6-2.4 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human Wnt-11 protein
Ile25-Lys354
Accession #
NP_004617
N-terminal Sequence
Analysis
Ile25
Predicted Molecular Mass
36.6 kDa
SDS-PAGE
38-40 kDa, reducing conditions

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6179-WN (with carrier)

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6179-WN/CF (carrier free)

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COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6179-WN

Formulation Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

6179-WN/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Wnt-11

Wnt‑11 belongs to the Wnt family of secreted, highly conserved, cysteine-rich cell signaling glycoproteins that play important roles in vertebrate developmental pattern formation, cell fate decision, axon guidance, and tumor formation (1-3). Mature human Wnt‑11 shares 98% amino acid (aa) sequence identity with mouse, rat and bovine Wnt‑11, and 99%, 96% and 88% aa identity with canine, equine and chick Wnt‑11, respectively. Mouse Wnt‑11 is expressed in early development during gastrulation and in the perichondrium, lung mesenchyme and ureteric buds, but not in neuroepithelium (1). It is required for cardiogenesis, organization of early muscle fibers, and branching morphogenesis of ureters (3-7). It is also expressed in several human cancers (2). In vitro, Wnt‑11 has been shown to induce cardiomyocyte differentiation from unfractionated mouse bone marrow mononuclear cells, and to promote hematopoietic differentiation from human embryoid body stem cells by engaging Frizzled-7 in the presence of E-cadherin (6, 8). In general, there are three signaling pathways associated with Wnt‑receptor interaction. The canonical pathway results in beta -Catenin accumulation and TCF/LEF‑1‑mediated gene transcription. Non‑canonical pathways include the Wnt/Ca2+ pathway and the planar cell polarity (PCP) pathway. Wnt‑11 often, but not exclusively, signals by a non‑canonical pathway and may repress canonical signaling by other Wnts (2, 9). However, when complexed with Wnt‑5a, Wnt‑11 can also enhance canonical signaling (10).

References
  1. Lako, M. et al. (1998) Gene 219:101.
  2. Katoh, M. and M. Katoh (2009) Int. J. Mol. Med. 24:247.
  3. Flaherty, M.P. and B. Dawn (2008) Trends Cardiovasc. Med. 18:260.
  4. Gros, J. et al. (2009) Nature 457:589.
  5. Pandur, P. et al. (2002) Nature 418:636.
  6. Flaherty, M.P. et al. (2008) Circulation 117:2241.
  7. Majumdar, A. et al. (2003) Development 130:3175.
  8. Vijayaragavan, K. et al. (2009) Cell Stem Cell 4:248.
  9. Maye, P. et al. (2004) J. Biol. Chem. 279:24659.
  10. Cha, S-W. et al. (2009) Curr. Biol. 19:1573.
Long Name
Wingless-type MMTV Integration Site Family, Member 11
Entrez Gene IDs
7481 (Human); 22411 (Mouse)
Alternate Names
HWNT11; MGC141946; MGC141948; protein Wnt-11; wingless-type MMTV integration site family, member 11; Wnt11; Wnt-11

Citations for Recombinant Human Wnt-11 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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  1. New mutation in the ?1 propeller domain of LRP4 responsible for congenital myasthenic syndrome associated with Cenani-Lenz syndrome
    Authors: Masingue, M;Cattaneo, O;Wolff, N;Buon, C;Sternberg, D;Euchparmakian, M;Boex, M;Behin, A;Mamchaouhi, K;Maisonobe, T;Nougues, MC;Isapof, A;Fontaine, B;Messéant, J;Eymard, B;Strochlic, L;Bauché, S;
    Scientific reports
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Adult Multipotent Cardiac Progenitor-Derived Spheroids: A Reproducible Model of In Vitro Cardiomyocyte Commitment and Specification
    Authors: Scalise, M;Marino, F;Salerno, L;Amato, N;Quercia, C;Siracusa, C;Filardo, A;Chiefalo, A;Pagano, L;Misdea, G;Salerno, N;De Angelis, A;Urbanek, K;Viglietto, G;Torella, D;Cianflone, E;
    Cells
    Species: Mouse
    Sample Types: Spheroid
    Applications: Bioassay
  3. Pharmacological clearance of senescent cells improves cardiac remodeling and function after myocardial infarction in female aged mice
    Authors: N Salerno, F Marino, M Scalise, L Salerno, C Molinaro, A Filardo, A Chiefalo, G Panuccio, A De Angelis, K Urbanek, D Torella, E Cianflone
    Mechanisms of Ageing and Development, 2022-09-20;208(0):111740.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Differentiation, Differentiation
  4. Wnt11 acts on dermomyotome cells to guide epaxial myotome morphogenesis
    Authors: AK Heilig, R Nakamura, A Shimada, Y Hashimoto, Y Nakamura, J Wittbrodt, H Takeda, T Kawanishi
    Elife, 2022-05-06;11(0):.
    Species: Zebrafish
    Sample Types: In Vivo
    Applications: In Vivo
  5. Comparison of Differentiation Pattern and WNT/SHH Signaling in Pluripotent Stem Cells Cultured under Different Conditions
    Authors: B ?wierczek-, D Dudka, D Bauer, T Czajkowski, K Ilach, W Streminska, A Kominek, K Piwocka, MA Ciemerych, K Archacka
    Cells, 2021-10-14;10(10):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Bioassay
  6. MiR-21 Is Required for the Epithelial-Mesenchymal Transition in MDA-MB-231 Breast Cancer Cells
    Authors: ED Arisan, O Rencuzogul, C Cieza-Borr, F Miralles A, M Dwek, S Lange, P Uysal-Onga
    International Journal of Molecular Sciences, 2021-02-04;22(4):.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  7. Non-canonical Wnt/PCP signalling regulates intestinal stem cell lineage priming towards enteroendocrine and Paneth cell fates
    Authors: A Böttcher, M Büttner, S Tritschler, M Sterr, A Aliluev, L Oppenlände, I Burtscher, S Sass, M Irmler, J Beckers, C Ziegenhain, W Enard, AC Schamberge, FM Verhamme, O Eickelberg, FJ Theis, H Lickert
    Nature Cell Biology, 2021-01-04;23(1):23-31.
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  8. WNT11-FZD7-DAAM1 signalling supports tumour initiating abilities and melanoma amoeboid invasion
    Authors: I Rodriguez-, O Maiques, L Kohlhammer, G Cantelli, A Perdrix-Ro, J Monger, B Fanshawe, VL Bridgeman, SN Karagianni, RM Penin, J Marcolval, RM Marti, X Matias-Gui, GO Fruhwirth, JL Orgaz, I Malanchi, V Sanz-Moren
    Nat Commun, 2020-10-20;11(1):5315.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Canonical Wnt signaling promotes pacemaker cell specification of cardiac mesodermal cells derived from mouse and human embryonic stem cells
    Authors: W Liang, P Han, EH Kim, J Mak, R Zhang, AG Torrente, JI Goldhaber, E Marbán, HC Cho
    Stem Cells, 2019-12-30;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Precardiac organoids form two heart fields via Bmp/Wnt signaling
    Authors: P Andersen, E Tampakakis, DV Jimenez, S Kannan, M Miyamoto, HK Shin, A Saberi, S Murphy, E Sulistio, SP Chelko, C Kwon
    Nat Commun, 2018-08-07;9(1):3140.
    Species: Mouse
    Sample Types:
  11. Role of Wnt11 during Osteogenic Differentiation of Human Mesenchymal Stem Cells on Microstructured Titanium Surfaces
    Authors: BD Boyan, R Olivares-N, MB Berger, SL Hyzy, Z Schwartz
    Sci Rep, 2018-06-05;8(1):8588.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Wnt inhibition promotes vascular specification of embryonic cardiac progenitors
    Authors: DE Reichman, L Park, L Man, D Redmond, K Chao, RP Harvey, MM Taketo, Z Rosenwaks, D James
    Development, 2018-01-08;145(1):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Differentiation, Differentiation
  13. Wnt11 regulates cardiac chamber development and disease during perinatal maturation
    Authors: M Touma, X Kang, F Gao, Y Zhao, AA Cass, R Biniwale, X Xiao, M Eghbali, G Coppola, B Reemtsen, Y Wang
    JCI Insight, 2017-09-07;2(17):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Wnts contribute to neuromuscular junction formation through distinct signaling pathways
    Authors: J Messéant, J Ezan, P Delers, K Glebov, C Marchiol, F Lager, G Renault, F Tissir, M Montcouqui, N Sans, C Legay, L Strochlic
    Development, 2017-03-27;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  15. Increased Wnt5a in squamous cell lung carcinoma inhibits endothelial cell motility
    BMC Cancer, 2016-11-23;16(1):915.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Switch from canonical to noncanonical Wnt signaling mediates high glucose-induced adipogenesis.
    Authors: Keats E, Dominguez J, Grant M, Khan Z
    Stem Cells, 2014-06-01;32(6):1649-60.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Assessment of Frizzled 6 membrane mobility by FRAP supports G protein coupling and reveals WNT-Frizzled selectivity.
    Authors: Kilander M, Dahlstrom J, Schulte G
    Cell Signal, 2014-05-27;26(9):1943-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Activation of intracellular calcium by multiple Wnt ligands and translocation of beta-catenin into the nucleus: a convergent model of Wnt/Ca2+ and Wnt/beta-catenin pathways.
    Authors: Thrasivoulou C, Millar M, Ahmed A
    J Biol Chem, 2013-10-24;288(50):35651-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012-07-26;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Wnt5a and Wnt11 are essential for second heart field progenitor development.
    Authors: Cohen E, Miller M, Wang Z, Moon R, Morrisey E
    Development, 2012-06-01;139(11):1931-40.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Human Wnt-11 Protein
By Anonymous on 10/09/2020
Application: CellProlif