Recombinant Human VISTA/B7-H5/PD-1H Fc Avi-tag Protein, CF

Catalog #: AVI7126 Datasheet / COA / SDS

Biotinylated

Catalog #	Availability	Size / Price	Qty
AVI7126-050

Recombinant Human VISTA/B7-H5/PD-1H Fc Avi-tag Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human VISTA/B7-H5/PD-1H Fc Avi-tag Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Why choose R&D Systems Avi-tag VISTA Protein?

Guaranteed Bioactivity and High Purity: Bioactivity tested by functional ELISA and purity determined by SDS-PAGE to be greater than 95%.
Lot-to-Lot Consistency: Stringent QC testing performed on each lot to ensure consistent activity and purity.
Bulk Quantities Available: Bulk up and save with large mass quantities to meet your research needs. Supply agreements available, partner with us. Please contact us.
Most Respected, Most Cited Brand in Proteins: With over 35 years of providing the best recombinant proteins to the scientific community, R&D Systems continues to lead the industry in quality, activity, and purity.

Find the His Avi-tag VISTA protein here His Avi-tag VISTA Protein

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human VSIG3 Fc Chimera (Catalog # 9229-VS) is immobilized at 5 µg/mL (100 µL/well), Biotinylated Recombinant Human VISTA/B7-H5/PD-1H Fc Chimera Avi-tag (Catalog # AVI7126) binds with an ED₅₀ of 0.5-3 μg/mL.

Source

Human embryonic kidney cell, HEK293-derived human VISTA/B7-H5/PD-1H protein

Human VISTA/B7-H5/PD-1H (Phe33-Ala194) Accession # AAH20568	IEGRMD	Human IgG₁ (Pro100-Lys330)	Avi-tag
N-terminus			C-terminus

Accession #

AAH20568

N-terminal Sequence
Analysis

Phe33

Structure / Form

Disulfide-linked homodimer, biotinylated protein via Avi-tag

Predicted Molecular Mass

47 kDa

SDS-PAGE

64-75 kDa, under reducing conditions

Product Datasheets

AVI7126

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI7126

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human VSIG3 Fc Chimera (Catalog # 9229-VS) is coated at 5 µg/mL, Biotinylated Recombinant Human VISTA/B7-H5/PD-1H Fc Chimera Avi-tag (Catalog # AVI7126) binds with an ED₅₀ of 0.5-3 µg/mL.

SDS-PAGE

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2 μg/lane of Biotinylated Recombinant Human VISTA/B7-H5/PD-1H Fc Chimera Avi-tag (Catalog # AVI7126) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 64-75 kDa and 130-150 kDa, respectively.

Reconstitution Calculator

Background: VISTA/B7-H5/PD-1H

Platelet receptor Gi24, also known as Dies1, VISTA, SISP1 and B7‑H5, is a 55‑65 kDa transmembrane glycoprotein with homology to B7‑like immune co‑stimulatory molecules (1, 2). Mature human Gi24 contains a 162 amino acid (aa) extracellular domain (ECD) with one V‑type Ig‑like domain, a 21 aa transmembrane segment, and a 96 aa cytoplasmic domain. Within the ECD, human Gi24 shares 70% and 67% aa sequence identity with mouse and rat Gi24, respectively (3). The 30 kDa ECD can be shed by MT1‑MMP, with a 25‑30 kDa fragment remaining in the membrane (3). Gi24 promotes both MT1‑MMP expression and the MT1‑MMP mediated activation of MMP‑2 (3). Gi24 supports the differentiation of embryonic stem cells (ESC) and enhances BMP‑4 induced signaling in ESC, but is also down‑regulated following BMP‑4 exposure (4, 5). It binds to BMP‑4 directly, and also associates with the type I BMP receptor Activin RIB/ALK‑4 (4, 5). Gi24 is expressed on the surface of naïve CD4⁺ T cells and regulatory T cells (6). It is up‑regulated in vivo on activated monocytes and dendritic cells (5). Gi24 inhibits CD4⁺ and CD8⁺ T cell proliferation, and their production of IL‑2 and IFN‑ gamma (6). Its expression on tumor cells attenuates the anti‑tumor immune response and enables more rapid tumor progression (6). In contrast, Gi24 limits disease progression in the autoimmune disease model EAE (6).

References

Flajnik, M.F. et al. (2012) Immunogenetics 64:571.
Wilcox, R.A. et al. (2012) Eur. J. Haematol. 88:465.
Sakr, M.A. et al. (2010) Cancer Sci. 101:2368.
Aloia, L. et al. (2010) J. Biol. Chem. 285:7776.
Parisi, S. et al. (2012) FASEB J. 26:3957.
Wang, L. et al. (2011) J. Exp. Med. 208:577.

Entrez Gene IDs

64115 (Human); 74048 (Mouse); 690899 (Rat); 102116468 (Cynomolgus Monkey)

Alternate Names

4632428N05Rik; B7H5; B7-H5; C10orf54; chromosome 10 open reading frame 54; Dies1; Gi24; PD1H; PD-1H; platelet receptor Gi24; PP2135; SISP1; stress induced secreted protein 1; VISTA; VSIR