Recombinant Human VEGFR2/KDR Fc Chimera Protein

Name: Recombinant Human VEGFR2/KDR Fc Chimera Protein
Brand: R&D Systems
SKU: 357-KD
Rating: 5 (1 reviews)

Catalog #: 357-KD
41 Citations 1 Review Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
357-KD-050/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
357-KD-050

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Product Details

Citations (41)

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Reviews (1)

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human VEGFR2/KDR Fc Chimera Protein Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit the VEGF-dependent proliferation of HUVEC human umbilical vein endothelial cells. The ED₅₀ for this effect is 10-40 ng/mL in the presence of 10 ng/mL recombinant human VEGF₁₆₅.

Source

Mouse myeloma cell line, NS0-derived human VEGFR2/KDR/Flk-1 protein

Human VEGFR2 (Ala20-Glu764) Accession # AAC16450	IEGRMD	Human IgG₁ (Pro100-Lys330)	6-His tag
N-terminus			C-terminus

Accession #

AAC16450

N-terminal Sequence
Analysis

Ala20

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

110 kDa (monomer)

SDS-PAGE

160-170 kDa, reducing conditions

Product Datasheets

357-KD (with carrier)

Product Datasheet

COA

SDS

357-KD/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

357-KD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

357-KD/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: VEGFR2/KDR/Flk-1

VEGFR2 (KDR/Flk-1), VEGFR1 (Flt-1) and VEGFR3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGFR1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis.

VEGFR2 cDNA encodes a 1356 amino acid (aa) residue precursor protein with a 19 aa residue signal peptide. Mature VEGFR2 is composed of a 745 aa residue extracellular domain, a 25 aa residue transmembrane domain and a 567 aa residue cytoplasmic domain. In contrast to VEGFR1 which binds both PlGF and VEGF with high affinity, VEGFR2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGFR2/Fc chimera binds VEGF with high affinity and is a potent VEGF antagonist.

References

Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.

Long Name

Vascular Endothelial Growth Factor Receptor 2

Entrez Gene IDs

3791 (Human); 16542 (Mouse)

Alternate Names

CD309 antigen; CD309; EC 2.7.10; EC 2.7.10.1; Fetal liver kinase 1; fetal liver kinase-1; Flk1; Flk-1; FLK1tyrosine kinase growth factor receptor; KDR; kinase insert domain receptor (a type III receptor tyrosine kinase); Kinase insert domain receptor; KRD1; Ly73; Protein-tyrosine kinase receptor flk-1; soluble VEGFR2; vascular endothelial growth factor receptor 2; VEGF R2; VEGFR; VEGFR2; VEGFR-2

Related Research Areas

Citations for Recombinant Human VEGFR2/KDR Fc Chimera Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

41 Citations: Showing 1 - 10
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A Novel Blood-Brain Barrier-Penetrating and Vascular-Targeting Chimeric Peptide Inhibits Glioma Angiogenesis
Authors: Lu, L;Wang, L;Zhao, L;Liao, J;Zhao, C;Xu, X;Wang, F;Zhang, X;
International journal of molecular sciences
Species: N/A
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance (SPR)
Reconstruction of distinct vertebrate gastrulation modes via modulation of key cell behaviors in the chick embryo
Authors: M Chuai, G Serrano Ná, M Serra, L Mahadevan, CJ Weijer
Science Advances, 2023-01-04;9(1):eabn5429.
Species: Chick
Sample Types: Embryos
Applications: Bioassay
Optimized Heterologous Expression and Efficient Purification of a New TRAIL-Based Antitumor Fusion Protein SRH-DR5-B with Dual VEGFR2 and DR5 Receptor Specificity
Authors: AV Yagolovich, AA Artykov, AA Isakova, YV Vorontsova, DA Dolgikh, MP Kirpichnik, ME Gasparian
International Journal of Molecular Sciences, 2022-05-24;23(11):.
Species: Human
Sample Types: Protein
Applications: ELISA Capture
A Dual Receptor Targeting- and BBB Penetrating- Peptide Functionalized Polyethyleneimine Nanocomplex for Secretory Endostatin Gene Delivery to Malignant Glioma
Authors: L Lu, H Chen, L Wang, L Zhao, Y Cheng, A Wang, F Wang, X Zhang
Int J Nanomedicine, 2020-11-12;15(0):8875-8892.
Species: Human
Sample Types: Protein
Applications: SPR
Predicting the Efficacy and Safety of TACTICs (Tumor Angiogenesis-Specific CAR-T Cells Impacting Cancers) Therapy for Soft Tissue Sarcoma Patients
Authors: K Fujiwara, S Sasawatari, S Nakai, K Imaeda, S Nagai, Y Matsuno, K Hatanaka, Y Hatanaka, S Takenaka, N Okada
Cancers, 2020-09-23;12(10):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
VEGF-A splice variants bind VEGFRs with differential affinities
Authors: SB Mamer, A Wittenkell, PI Imoukhuede
Sci Rep, 2020-09-02;10(1):14413.
Species: Human, N/A
Sample Types: Protein, Recombinant Protein
Applications: Surface Plasmon Resonance, Bioassay
Streamlined human antibody generation and optimization by exploiting designed immunoglobulin loci in a B cell line
Authors: H Seo, H Masuda, K Asagoshi, T Uchiki, S Kawata, G Sasaki, T Yabuki, S Miyai, N Takahashi, SI Hashimoto, A Sawada, A Takaiwa, C Koyama, K Tamai, K Kurosawa, KY Lin, K Ohta, Y Nakazaki
Cell. Mol. Immunol., 2020-05-26;0(0):.
Species: Mouse
Sample Types:
Calcium dobesilate reduces VEGF signaling by interfering with heparan sulfate binding site and protects from vascular complications in diabetic mice
Authors: F Njau, N Shushakova, H Schenk, VC Wulfmeyer, R Bollin, J Menne, H Haller
PLoS ONE, 2020-01-14;15(1):e0218494.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
VEGF-A-Cleavage by FSAP and Inhibition of Neo-Vascularization
Authors: Ö Uslu, J Herold, S M Kanse
Cells, 2019-11-06;8(11):.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay
Hypoxia Induced Heparan Sulfate Primes the Extracellular Matrix for Endothelial Cell Recruitment by Facilitating VEGF-Fibronectin Interactions
Authors: JA Buczek-Tho, CB Rich, MA Nugent
Int J Mol Sci, 2019-10-12;20(20):.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay

Enhancement of HIFU ablation by sonosensitizer-loading liquid fluorocarbon nanoparticles with pre-targeting in a mouse model
Authors: Y Zhang, L Yong, Y Luo, X Ding, D Xu, X Gao, S Yan, Q Wang, J Luo, D Pu, J Zou
Sci Rep, 2019-05-06;9(1):6982.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

A 3D Human Renal Cell Carcinoma-on-a-Chip for the Study of Tumor Angiogenesis
Authors: CP Miller, C Tsuchida, Y Zheng, J Himmelfarb, S Akilesh
Neoplasia, 2018-05-07;20(6):610-620.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

ABT-165, a Dual Variable Domain Immunoglobulin (DVD-Ig) Targeting DLL4 and VEGF, Demonstrates Superior Efficacy and Favorable Safety Profiles in Preclinical Models
Authors: Y Li, JA Hickson, DJ Ambrosi, DL Haasch, KD Foster-Duk, LJ Eaton, EL Digiammari, SC Panchal, F Jiang, SR Mudd, C Zhang, SS Akella, W Gao, SL Ralston, L Naumovski, J Gu, SE Morgan-Lap
Mol. Cancer Ther., 2018-03-28;0(0):.
Applications: ELISA (Standard)

Discovery of High-Affinity PDGF-VEGFR Interactions: Redefining RTK Dynamics
Authors: SB Mamer, S Chen, JC Weddell, A Palasz, A Wittenkell, M Kumar, PI Imoukhuede
Sci Rep, 2017-11-27;7(1):16439.
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance

Secretogranin III as a disease-associated ligand for antiangiogenic therapy of diabetic retinopathy
Authors: ME LeBlanc, W Wang, X Chen, NB Caberoy, F Guo, C Shen, Y Ji, H Tian, H Wang, R Chen, W Li
J. Exp. Med, 2017-03-22;0(0):.
Applications: Bioassay

Ultrasound Molecular Imaging of Angiogenesis Using Vascular Endothelial Growth Factor-Conjugated Microbubbles
Authors: J Wang, B Qin, X Chen, WR Wagner, FS Villanueva
Mol. Pharm., 2017-02-15;14(3):781-790.
Applications: Bioassay

Generation and characterization of ABBV642, a dual variable domain immunoglobulin molecule (DVD-Ig) that potently neutralizes VEGF and PDGF-BB and is designed for the treatment of exudative age-related macular degeneration
Authors: Kun Ding
MAbs, 2017-02-01;0(0):0.
Applications: Bioassay

Differential receptor binding and regulatory mechanisms for the lymphangiogenic growth factors VEGF-C and VEGF-D
Authors: Marc G Achen
J. Biol. Chem., 2016-11-16;0(0):.
Applications: Bioassay

Immunological quality and performance of tumor vessel-targeting CAR-T cells prepared by mRNA-EP for clinical research
Authors: Naoki Okada
Mol Ther Oncolytics, 2016-11-16;3(0):16024.
Species: Mouse
Sample Types: Complex Sample Type
Applications: Bioassay

Proangiogenic Interactions of Vascular Endothelial MMP14 With VEGF Receptor 1 in VEGFA-Mediated Corneal Angiogenesis.
Authors: Kyu-Yeon Han, Jin-Hong Chang, Hyun Lee, Dimitri T Azar
Investigative Ophthalmology & Visual Science, 2016-06-01;0(0):1552-5783.
Species: Human
Sample Types: Recombinant Protein
Applications: Enzyme Assay

Synergistic Binding of Vascular Endothelial Growth Factor-A and Its Receptors to Heparin Selectively Modulates Complex Affinity.
Authors: Teran M, Nugent M
J Biol Chem, 2015-05-15;290(26):16451-62.
Species: Human
Sample Types: Protein
Applications: Bioassay

Soluble CD54 induces human endothelial cells ex vivo expansion useful for cardiovascular regeneration and tissue engineering application.
Authors: Malara N, Trunzo V, Musolino G, Aprigliano S, Rotta G, Macrina L, Limongi T, Gratteri S, Di Fabrizio E, Renzulli A, Fini M, Mollace V
Int J Cardiol Heart Vasc, 2015-01-09;6(0):48-53.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Simultaneous targeting of two ligand-binding sites on VEGFR2 using biparatopic Affibody molecules results in dramatically improved affinity.
Authors: Fleetwood F, Klint S, Hanze M, Gunneriusson E, Frejd F, Stahl S, Lofblom J
Sci Rep, 2014-12-17;4(0):7518.
Applications: Bioassay

Bone marrow-derived mesenchymal stem cells drive lymphangiogenesis.
Authors: Maertens L, Erpicum C, Detry B, Blacher S, Lenoir B, Carnet O, Pequeux C, Cataldo D, Lecomte J, Paupert J, Noel A
PLoS ONE, 2014-09-15;9(9):e106976.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Tissue inhibitor of metalloproteinases-3 peptides inhibit angiogenesis and choroidal neovascularization in mice.
Authors: Qi, Jian Hua, Ebrahem, Quteba, Ali, Mariya, Cutler, Alecia, Bell, Brent, Prayson, Nicholas, Sears, Jonathan, Knauper, Vera, Murphy, Gillian, Anand-Apte, Bela
PLoS ONE, 2013-03-01;8(3):e55667.
Species: Human
Sample Types: Recombinant Protein
Applications: ELISA

The propeptides of VEGF-D determine heparin binding, receptor heterodimerization, and effects on tumor biology.
Authors: Harris, Nicole C, Davydova, Natalia, Roufail, Sally, Paquet-Fifield, Sophie, Paavonen, Karri, Karnezis, Tara, Zhang, You-Fang, Sato, Teruhiko, Rothacker, Julie, Nice, Edouard, Stacker, Steven A, Achen, Marc G
J Biol Chem, 2013-02-12;288(12):8176-86.
Species: Human
Sample Types: Protein
Applications: Bioassay

Vascular endothelial growth factor signals through platelet-derived growth factor receptor beta in meningiomas in vitro.
Br. J. Cancer, 2012-10-09;107(10):1702-13.
Applications: Bioassay

MULTIMERIN2 impairs tumor angiogenesis and growth by interfering with VEGF-A/VEGFR2 pathway.
Authors: Lorenzon E, Colladel R, Andreuzzi E, Marastoni S, Todaro F, Schiappacassi M, Ligresti G, Colombatti A, Mongiat M
Oncogene, 2011-10-24;31(26):3136-47.
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance

Endorepellin, the angiostatic module of perlecan, interacts with both the alpha2beta1 integrin and vascular endothelial growth factor receptor 2 (VEGFR2): a dual receptor antagonism.
Authors: Goyal A, Pal N, Concannon M, Paul M, Doran M, Poluzzi C, Sekiguchi K, Whitelock JM, Neill T, Iozzo RV
J. Biol. Chem., 2011-05-19;286(29):25947-62.
Species: Human
Sample Types: Recombinant Protein
Applications: Binding Assay

Regulation of pathologic retinal angiogenesis in mice and inhibition of VEGF-VEGFR2 binding by soluble heparan sulfate.
Authors: Nishiguchi KM, Kataoka K, Kachi S
PLoS ONE, 2010-10-20;5(10):e13493.
Species: Porcine
Sample Types: Aqueous Humor
Applications: Bioassay

Soluble forms of VEGF receptor-1 and -2 promote vascular maturation via mural cell recruitment.
Authors: Lorquet S, Berndt S, Blacher S, Gengoux E, Peulen O, Maquoi E, Noel A, Foidart JM, Munaut C, Pequeux C
FASEB J., 2010-05-19;24(10):3782-95.
Species: Human, Mouse
Sample Types: Whole Cells
Applications: Bioassay, IHC

VEGF(121)b, a new member of the VEGF(xxx)b family of VEGF-A splice isoforms, inhibits neovascularisation and tumour growth in vivo.
Authors: Rennel ES, Varey AH, Churchill AJ, Wheatley ER, Stewart L, Mather S, Bates DO, Harper SJ
Br. J. Cancer, 2009-08-25;101(7):1183-93.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay

Modulation of angiogenesis by a tetrameric tripeptide that antagonizes vascular endothelial growth factor receptor 1.
Authors: Ponticelli S, Marasco D, Tarallo V, Albuquerque RJ, Mitola S, Takeda A, Stassen JM, Presta M, Ambati J, Ruvo M, De Falco S
J. Biol. Chem., 2008-10-15;283(49):34250-9.
Species: Human
Sample Types: Peptide
Applications: Binding Assay

Neuropilin-1 binds to VEGF121 and regulates endothelial cell migration and sprouting.
Authors: Pan Q, Chathery Y, Wu Y, Rathore N, Tong RK, Peale F, Bagri A, Tessier-Lavigne M, Koch AW, Watts RJ
J. Biol. Chem., 2007-06-16;282(33):24049-56.
Species: Human
Sample Types: Recombinant Protein
Applications: Binding Assay

Distinct vascular endothelial growth factor signals for lymphatic vessel enlargement and sprouting.
Authors: Wirzenius M, Tammela T, Uutela M, He Y, Odorisio T, Zambruno G, Nagy JA, Dvorak HF, Yla-Herttuala S, Shibuya M, Alitalo K
J. Exp. Med., 2007-05-29;204(6):1431-40.
Species: Porcine
Sample Types: Whole Cells
Applications: Bioassay

Molecular imaging of VEGF receptors in angiogenic vasculature with single-chain VEGF-based probes.
Authors: Backer MV, Levashova Z, Patel V, Jehning BT, Claffey K, Blankenberg FG, Backer JM
Nat. Med., 2007-03-11;13(4):504-9.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Low molecular weight fucoidan increases VEGF165-induced endothelial cell migration by enhancing VEGF165 binding to VEGFR-2 and NRP1.
Authors: Lake AC, Vassy R, Di Benedetto M, Lavigne D, Le Visage C, Perret GY, Letourneur D
J. Biol. Chem., 2006-10-06;281(49):37844-52.
Species: Human
Sample Types: Buffer
Applications: Bioassay

Association between UHMWPE particle-induced inflammatory osteoclastogenesis and expression of RANKL, VEGF, and Flt-1 in vivo.
Authors: Ren WP, Markel DC, Zhang R, Peng X, Wu B, Monica H, Wooley PH
Biomaterials, 2006-06-30;27(30):5161-9.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Antagonists to human and mouse vascular endothelial growth factor receptor 2 generated by directed protein evolution in vitro.
Authors: Getmanova EV, Chen Y, Bloom L, Gokemeijer J, Shamah S, Warikoo V, Wang J, Ling V, Sun L
Chem. Biol., 2006-05-01;13(5):549-56.
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance

Suppression of angiogenesis and tumor growth by selective inhibition of angiopoietin-2.
Authors: Oliner J, Min H, Leal J, Yu D, Rao S, You E, Tang X, Kim H, Meyer S, Han SJ, Hawkins N, Rosenfeld R, Davy E, Graham K, Jacobsen F, Stevenson S, Ho J, Chen Q, Hartmann T, Michaels M, Kelley M, Li L, Sitney K, Martin F, Sun JR, Zhang N, Lu J, Estrada J, Kumar R, Coxon A, Kaufman S, Pretorius J, Scully S, Cattley R, Payton M, Coats S, Nguyen L, Desilva B, Ndifor A, Hayward I, Radinsky R, Boone T, Kendall R
Cancer Cell, 2004-11-01;6(5):507-16.
Species: Human
Sample Types:
Applications: Functional ELISA

Estrogen enhances angiogenesis through a pathway involving platelet-activating factor-mediated nuclear factor-kappaB activation.
Authors: Seo KH, Lee HS, Jung B, Ko HM, Choi JH, Park SJ, Choi IH, Lee HK, Im SY
Cancer Res., 2004-09-15;64(18):6482-8.
Species: Porcine
Sample Types: Whole Cells
Applications: Bioassay