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Recombinant Human UBE2K/E2-25K Protein, CF

Catalog #: E2-603
1 Citation Datasheet / COA / SDS

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Catalog # Availability Size / Price Qty
E2-603-100
R&D Systems Recombinant Proteins and Enzymes
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human UBE2K/E2-25K Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UBE2K/E2-25K concentration of 0.1-1 μM.
Source
E. coli-derived human UBE2K/E2-25K protein
Met1 - Asn200
Accession #
P61086.3
Predicted Molecular Mass
22 kDa

Product Datasheets

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E2-603

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E2-603

Formulation Supplied as a solution in HEPES, NaCl and TCEP.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: UBE2K/E2-25K

Ubiquitin-conjugating Enzyme E2K (UBE2K), also known as E2-25K, HIP2, and LIG, is a 200 amino acid (aa) protein with a predicted molecular weight of 25 kDa. Human and mouse UBE2K share 100% aa sequence identity. Similar to other Ubiquitin-conjugating (E2) enzymes, UBE2K/E2-25K contains a conserved Ubiquitin-conjugating domain but is unique in that it also has a C-terminal, non-covalent Ubiquitin binding surface termed the Ubiquitin-associated domain between aa residues 160 and 200 (1,2). Recombinant Human UBE2K/E2-25K is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins; UBE2K/E2-25K mediates the elongation of Lys48-linked poly-Ubiquitin chains (3). UBE2K/E2-25K catalytic activity can be modulated by the post-translational addition of SUMO at Lys14 (4). Substrates include the Huntingtin protein and the tumor suppressor RB1 (5,6). UBE2K/E2-25K is widely expressed, with highest levels found in the brain cortex and striatum, and dysregulated UBE2K/E2-25K is implicated in polyglutamine diseases and Alzheimer's disease (7-9).

References
  1. Wilson, R.C. et al. (2011) Biochem. Biophys. Res. Commun. 405:662.
  2. Haldeman, M.T. et al. (1997) Biochemistry 36:10526.
  3. Chen, Z.J. et al. (1991) J. Biol. Chem. 266:15698.
  4. Pickler, A. et al. (2005) Nat. Struct. Mol. Biol. 12:264.
  5. Kalchman, M.A. et al. (1996) J. Biol. Chem. 271:19385.
  6. Oh, K.J. et al. (2010) Virology 396:118.
  7. de Pril, R. et al. (2007) Mol. Cell. Neurosci. 34:10.
  8. Skibinski, G.A. & L. Boyd (2012) BMC Cell Biol. 13:10.
  9. Song, S. et al. (2003) Mol. Cell 12:553.
Long Name
Ubiquitin-conjugating Enzyme E2
Entrez Gene IDs
3093 (Human); 53323 (Mouse); 289623 (Rat)
Alternate Names
E2(25K); E225K; E2-25K; EC 6.3.2.19; Hip2; HIP-2; huntingtin interacting protein 2; Huntingtin-interacting protein 2; HYPG; LIG; UbcH1; UBE2K; UBE2K-25K; ubiquitin carrier protein; ubiquitin-conjugating enzyme E2 K; Ubiquitin-conjugating enzyme E2(25K); Ubiquitin-conjugating enzyme E2-25 kDa; Ubiquitin-conjugating enzyme E2-25K; ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast); ubiquitin-protein ligase

Citation for Recombinant Human UBE2K/E2-25K Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. cIAP1-based degraders induce degradation via branched ubiquitin architectures
    Authors: Y Akizuki, M Morita, Y Mori, A Kaiho-Soma, S Dixit, A Endo, M Shimogawa, G Hayashi, M Naito, A Okamoto, K Tanaka, Y Saeki, F Ohtake
    Nature Chemical Biology, 2022-10-31;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay

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