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Recombinant Human UBE2I/Ubc9 Protein, CF

Catalog #: E2-645
8 Citations Datasheet / COA / SDS

Please inquire regarding future lot manufacture.

Will be discontinued when existing inventory is gone
Catalog # Availability Size / Price Qty
E2-645-100
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (8)
FAQs
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human UBE2I/Ubc9 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UBE2I/Ubc9 concentration of 0.1-1 μM.
Source
E. coli-derived human UBE2I/Ubc9 protein
Met1 - Ser158
Accession #
P63279.1
Predicted Molecular Mass
18 kDa

Product Datasheets

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E2-645

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E2-645

Formulation Supplied as a solution in HEPES, NaCl, Glycerol and TCEP.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: UBE2I/Ubc9

Ubiquitin-conjugating Enzyme E2I (UBE2I), also known as Ubiquitin-conjugating Enzyme 9 (Ubc9), is a ubiquitously expressed protein with a predicted molecular weight of 20 kDa. Human UBE2I/Ubc9 shares 100% amino acid sequence identity with the mouse and rat orthologs. UBE2I/Ubc9 catalyzes the addition of the Ubiquitin-like protein SUMO to target proteins (1,2). SUMO is transferred from a Ubiquitin-like activating (E1) enzyme heterodimer consisting of SAE1 and UBA2 to Cys93 of UBE2I/Ubc9 (3). In contrast to most Ubiquitin-conjugating (E2) enzymes that function in a complex with Ubiquitin ligases (E3s), UBE2I/Ubc9 can interact directly with protein substrates and may also play a role in substrate recognition (4). UBE2I/Ubc9 mediates the SUMOylation of a variety of proteins including RanGAP1, HDAC4, and PML (5,6). Post-translational modifications of UBE2I/Ubc9, such as auto-SUMOylation at Lys14 or Ser71 phosphorylation by CDC2/CDK1, alter UBE2I/Ubc9 catalytic activity and target protein recognition (5,7). UBE2I/Ubc9-dependent SUMOylation reduces the levels of the stem cell marker Nanog, implicating it in embryonic stem cell pluripotency maintenance (8). SUMOylation also regulates the protein levels of tumor suppressors and oncogenes, and UBE2I/Ubc9 dysregulation is thought to contribute to the pathogenesis of human cancers (9).

References
  1. Desterro, J.M. et al. (1999) J. Biol. Chem. 274:10618.
  2. Johnson, E.S. and G. Blobel (1997) J. Biol. Chem. 272:26799.
  3. Johnson, E.S. et al. (1997) EMBO J. 16:5509.
  4. Yunus, A.A. and C.D. Lima (2006) Nat. Struct. Mol. Biol. 13:491.
  5. Knipscheer, P. et al. (2008) Mol. Cell 31:371.
  6. Duprez, E. et al. (1999) J. Cell Sci. 112:381.
  7. Su, Y.F. et al. (2012) PLoS One 7:e34250.
  8. Wu, Y. et al. (2012) PLoS One 7:e39606.
  9. Mo, Y.Y. et al. (2005) Oncogene 24:2677.
Long Name
Ubiquitin-conjugating Enzyme E2I
Entrez Gene IDs
7329 (Human); 22196 (Mouse)
Alternate Names
C358B7.1; EC 6.3.2; EC 6.3.2.-; p18; SUMO-1-protein ligase; SUMO-protein Ligase; Ubc9; UBC9SUMO-conjugating enzyme UBC9; UBCE9; UBE2I; Ubiquitin carrier protein 9; Ubiquitin carrier protein I; ubiquitin conjugating enzyme 9; Ubiquitin-conjugating enzyme E2 I; ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9); ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast); ubiquitin-conjugating enzyme UbcE2A; ubiquitin-like protein SUMO-1 conjugating enzyme; ubiquitin-protein ligase E2I; Ubiquitin-protein ligase I

Citations for Recombinant Human UBE2I/Ubc9 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. The SUMO E3 ligase activity of ORF45 determines KSHV lytic replication
    Authors: Z Liu, X Wang, C Liu, H Deng, W Li, X Wang, X Xu, MZX Xiao, C Wang, Y Zhang, J Fu, F Zhu, Q Liang
    PloS Pathogens, 2022-04-28;18(4):e1010504.
    Species: Human
    Sample Types: Recombinant Proteins
    Applications: Bioassay
  2. RSK1 SUMOylation is required for KSHV lytic replication
    Authors: Z Liu, C Liu, X Wang, W Li, J Zhou, P Dong, MZX Xiao, C Wang, Y Zhang, J Fu, F Zhu, Q Liang
    PloS Pathogens, 2021-12-06;17(12):e1010123.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Mechanism of Adenovirus E4-ORF3-Mediated SUMO Modifications
    Authors: SY Sohn, P Hearing
    MBio, 2019-02-26;10(1):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Enzyme Assay
  4. TRIM28 promotes HIV-1 latency by SUMOylating CDK9 and inhibiting P-TEFb
    Authors: X Ma, T Yang, Y Luo, L Wu, Y Jiang, Z Song, T Pan, B Liu, G Liu, J Liu, F Yu, Z He, W Zhang, J Yang, L Liang, Y Guan, X Zhang, L Li, W Cai, X Tang, S Gao, K Deng, H Zhang
    Elife, 2019-01-17;8(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: SUMOylation Assay
  5. Cross-talk between chromatin acetylation and SUMOylation of tripartite motif-containing protein 24 (TRIM24) impacts cell adhesion
    Authors: S Appikonda, KN Thakkar, PK Shah, SYR Dent, JN Andersen, MC Barton
    J. Biol. Chem., 2018-03-09;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  6. Sumoylation in p27kip1 via RanBP2 promotes cancer cell growth in cholangiocarcinoma cell line QBC939
    Authors: J Yang, Y Liu, B Wang, H Lan, Y Liu, F Chen, J Zhang, J Luo
    BMC Mol. Biol., 2017-09-07;18(1):23.
    Species: Human
    Sample Types: Protein, Whole Cells
    Applications: Bioassay
  7. The adenovirus E4-ORF3 protein functions as a SUMO E3 ligase for TIF-1? sumoylation and poly-SUMO chain elongation
    Proc Natl Acad Sci USA, 2016-05-31;113(24):6725-30.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay

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