Recombinant Human TMIGD2/CD28H Fc Avi-tag Protein, CF

Catalog #: AVI8316 Datasheet / COA / SDS

Biotinylated Fc Chimera

Catalog #	Availability	Size / Price	Qty
AVI8316-050

Biotinylated Recombinant Human TMIGD2/CD28H Fc Chimera Avi-tag Protein Binding Activity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human TMIGD2/CD28H Fc Avi-tag Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human B7-H7 Fc Chimera (Catalog # 8084-B7) is immobilized at 1.00 μg/mL (100 μL/well), Biotinylated Recombinant Human TMIGD2/CD28H Fc Chimera Avi-tag (Catalog # AVI8316) binds with an ED₅₀ of 50.0-500 ng/mL

Source

Chinese Hamster Ovary cell line, CHO-derived human TMIGD2/CD28H protein

Human TMIGD2/CD28H (Leu23-Gly150) Accession # Q96BF3.2	IEGRMD	Human IgG₁ (Pro100-Lys330)	Avi-tag
N-terminus			C-terminus

Accession #

Q96BF3.2

N-terminal Sequence
Analysis

Leu23

Structure / Form

Disulfide-linked homodimer

Biotinylated via Avi-tag

Predicted Molecular Mass

43 kDa

SDS-PAGE

57-64 kDa, under reducing conditions.

Product Datasheets

AVI8316

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI8316

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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Measured by its binding ability in a functional ELISA. When Recombinant Human B7-H7 Fc Chimera (8084-B7) is immobilized at 1.00 μg/mL (100 μL/well), Biotinylated Recombinant Human TMIGD2/CD28H Fc Chimera Avi-tag Protein (Catalog # AVI8316) binds with an ED₅₀ of 50.0-500 ng/mL

SDS-PAGE

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2 μg/lane of Biotinylated Recombinant Human TMIGD2/CD28H Fc Chimera Avi-tag Protein (Catalog # AVI8316) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 57-64 kDa and 114-122 kDa, respectively.

Reconstitution Calculator

Background: TMIGD2/CD28H

CD28 homolog (CD28H), also called TMIG2 and IGPR-1, is a 55 kDa glycosylated transmembrane protein that shares approximately 10% amino acid (aa) sequence identity with CD28, CTLA-4, ICOS, and PD-1. CD28H is composed of a single extracellular immunoglobulin variable-like domain (IgV) (aa 23-109), a transmembrane domain (aa 151-171), and a long cytoplasmic domain (aa172-282). CD28H is constitutively expressed on naive T and NK cells. Similar to the interaction of B7 with CD28, the interaction of CD28H with B7-H7 activates the Akt-dependent signaling cascade and promotes the proliferation and activation of newly generated peripheral effector and memory T cells (1, 2). CD28H is additionally expressed in the skin and epithelium lining the lung, airway, mammary gland, and gastrointestinal tract (3). It regulates cellular morphology, focal adhesion contact formation, and cell migration (3, 4). CD28H also participates in angiogenesis in vitro (3). CD28H interacts with multiple cytoskeletal proteins including Actin, Paxillin, SPIN90, CACNB2, and BPAG1. Interactions between the cytoplasmic proline-rich domain of CD28H and SPIN90 modulate the activity of CD28H in both angiogenesis and cell adhesion (3, 5). Our Avi-tag Biotinylated human CD28H Fc chimera features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

Zhu, Y. et al. (2013) Nat. Commun. 4:2043.
Parry, R.V. et al. (2003) J.Immunol. 171:166.
Rahimi, N. et al. (2012) Mol. Biol. Cell. 23:1646.
Huang, C. et al. (2003) Nature 424:219.
Kaneko, T. et al. (2008) Front. Biosci. 13:4938.

Long Name

Transmembrane And Immunoglobulin Domain Containing 2

Entrez Gene IDs

126259 (Human)

Alternate Names

CD28H; IGPR1; IGPR-1; MGC23244; TMIGD2; transmembrane and immunoglobulin domain containing 2; transmembrane and immunoglobulin domain-containing protein 2